Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte.

Liu J, Guo C, Hao P, Wang P, Li L, Wang Y, Li X

BMC ophthalmology21 Jan 2022Cited by 1 (Europe PMC)

PMID35062902PMCIDPMC8783420DOI10.1186/s12886-022-02255-8PepBankPUB-35062902

Summary

PurposeTo investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).MethodsHCKs and BALB/c mice were chosen as the study subject. Ethanol was used to treat the cells and corneal stroma of mice to build the ethanol injured model in vitro and vivo respectively. CCK-8 was used to evaluate the cell metabolic activity. DCFH-DA was used to detect the intracellular reactive oxygen species level. TUNEL was chose to detect the cell apoptosis rate. The cell proliferation and migration were investigated by using wound healing insert. Wound healing of corneal surface and stroma was observed by using fluorescein sodium eyedrop and HE stain. RT-qPCR, ELISA, and immunostaining were performed to detect gene and protein expression in keratocytes or corneal stroma tissue of mice.ResultsEthanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3. Tβ4 promotes the proliferation of HCKs and the process of corneal wound healing. It may relevant to the up-regulated expression of Ki67.ConclusionsOur study established an ethanol-injured corneal stroma model in both vitro and vivo. The present study confirmed that Tβ4 play a protective effect on the reconstruction process of ethanol-injured corneal stroma.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In mice with ethanol-injured corneal stroma, Tβ4 promoted the process of corneal wound healing.
In plain wordsIn mice with an ethanol-injured cornea, Tβ4 helped the cornea heal.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCorneal wound healing
Study
mousen: not reported in source
ethanol-injured corneal stroma in BALB/c mice
Outcome group
Wound healing · Eye
Studied dose
Dose not stated
Source sentences
Summary
Tβ4 promotes the proliferation of HCKs and the process of corneal wound healing.
1 context sentence(design, population, supporting numbers)
Summary · context
Ethanol was used to treat the cells and corneal stroma of mice to build the ethanol injured model in vitro and vivo respectively.
Show in the study text Report a problemFND-3506290208
02LabIn vitro (cells)Efficacy outcome
In ethanol-injured human corneal keratocytes, Tβ4 alleviated oxidative stress injury.
In plain wordsIn cells, Tβ4 reduced oxidative stress injury caused by ethanol.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseEthanol-induced oxidative stress injury
Study
humann: not reported in source
ethanol-injured human corneal keratocytes (HCKs)
Outcome group
Oxidative stress · Eye
Studied dose
Dose not stated
Source sentences
Summary
Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290201
03LabIn vitro (cells)Efficacy outcome
In ethanol-injured human corneal keratocytes, Tβ4 alleviated cell apoptosis.
In plain wordsIn cells, Tβ4 reduced cell death caused by ethanol.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseEthanol-induced cell apoptosis
Study
humann: not reported in source
ethanol-injured human corneal keratocytes (HCKs)
Outcome group
Cell viability and death · Eye
Studied dose
Dose not stated
Source sentences
Summary
Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290202
04LabIn vitro (cells)Mechanism
In ethanol-injured human corneal keratocytes, Tβ4 up-regulated the expression of Bcl-2.
In plain wordsIn cells, Tβ4 raised Bcl-2 expression after ethanol injury.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseBcl-2 expression
Study
humann: not reported in source
ethanol-injured human corneal keratocytes (HCKs)
Outcome group
Receptor activity and mechanism · Eye
Studied dose
Dose not stated
Source sentences
Summary
Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290203
05LabIn vitro (cells)Mechanism
In ethanol-injured human corneal keratocytes, Tβ4 up-regulated the expression of catalase.
In plain wordsIn cells, Tβ4 raised catalase expression after ethanol injury.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCatalase expression
Study
humann: not reported in source
ethanol-injured human corneal keratocytes (HCKs)
Outcome group
Oxidative stress · Eye
Studied dose
Dose not stated
Source sentences
Summary
Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290204
06LabIn vitro (cells)Mechanism
In ethanol-injured human corneal keratocytes, Tβ4 up-regulated the expression of CuZnSOD.
In plain wordsIn cells, Tβ4 raised CuZnSOD expression after ethanol injury.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCuZnSOD expression
Study
humann: not reported in source
ethanol-injured human corneal keratocytes (HCKs)
Outcome group
Oxidative stress · Eye
Studied dose
Dose not stated
Source sentences
Summary
Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290205
07LabIn vitro (cells)Mechanism
In ethanol-injured human corneal keratocytes, Tβ4 inhibited the expression of Caspase-3.
In plain wordsIn cells, Tβ4 lowered Caspase-3 expression after ethanol injury.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseCaspase-3 expression
Study
humann: not reported in source
ethanol-injured human corneal keratocytes (HCKs)
Outcome group
Cell viability and death · Eye
Studied dose
Dose not stated
Source sentences
Summary
Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290206
08LabIn vitro (cells)Efficacy outcome
Tβ4 promoted the proliferation of human corneal keratocytes.
In plain wordsIn cells, Tβ4 increased the growth of corneal keratocytes.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseHCK proliferation
Study
humann: not reported in source
human corneal keratocytes (HCKs)
Outcome group
Cell proliferation and differentiation · Eye
Studied dose
Dose not stated
Source sentences
Summary
Tβ4 promotes the proliferation of HCKs and the process of corneal wound healing.
1 context sentence(design, population, supporting numbers)
Summary · context
To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs).
Show in the study text Report a problemFND-3506290207
09LabIn vitro (cells)Mechanism
The abstract links Tβ4-promoted proliferation of keratocytes to up-regulated expression of Ki67.
In plain wordsIn cells, the growth effect of Tβ4 may relate to higher Ki67 expression.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseKi67 expression
Study
humann: not reported in source
human corneal keratocytes (HCKs)
Outcome group
Cell proliferation and differentiation · Eye
Studied dose
Dose not stated
Source sentences
Summary
It may relevant to the up-regulated expression of Ki67.
Show in the study text Report a problemFND-3506290209

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

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