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Study · In vitro (cells) · onychostoma macrolepis · 2021

3 results

Functional Characterization of Melanocortin-3 Receptor in a Hibernating Cavefish Onychostoma macrolepis.

Wu L, Yu H, Mo H, Lan X, Pan C, Wang L, Zhao H, Zhou J, Li Y

Animals : an open access journal from MDPI25 Dec 2021Cited by 10 (Europe PMC)

PMID35011144 (opens in a new tab)PMCIDPMC8749556 (opens in a new tab)DOI10.3390/ani12010038 (opens in a new tab)PepBankPUB-35011144
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Summary

Melanocortin-3 receptor (MC3R) plays an important role in the energy homeostasis of animals under different nutritional conditions. Onychostoma macrolepis is a hibernating cavefish found in the northern part of the Yangtze River, and its adaptation to a nutrient-poor environment has attracted growing interest. In this study, we characterized the protein structure of Onychostoma macrolepis Mc3r (omMc3r), examined its tissue distribution, and investigated its function in mediating cellular signaling. We showed that the CDS of omMc3r is 978 bp, encoding a putative protein of 325 amino acids. Homology and phylogenetic analyses indicated that omMc3r is evolutionary close to cyprinids. Real-time quantitative PCR (RT-qPCR) revealed that omMc3r was highly expressed in the liver and brain. The functions of omMc3r to mediate ligands activating downstream signaling have also been confirmed by using signal pathway-specific reporters. The four agonists α-MSH, β-MSH, NDP-MSH, and ACTH (1-24) can all activate the cAMP and MAPK/ERK signaling pathway, albeit with different potency orders. The "primitive" ligand ACTH (1-24) had the highest potency on the cAMP signaling pathway, while the synthetic ligand NDP-MSH had the highest activation effect on the MAPK/ERK signaling pathway. This research will lay the foundation for studying the energy regulation mechanism of cavefish in an oligotrophic environment.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In cells expressing cavefish Mc3r (omMc3r), NDP-MSH activated the cAMP signaling pathway.

    In plain wordsIn cells carrying the cavefish Mc3r receptor, NDP-MSH switched on the cAMP signaling pathway.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasecAMP signaling activation via omMc3r
    Study

    Onychostoma macrolepisn: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The four agonists α-MSH, β-MSH, NDP-MSH, and ACTH (1-24) can all activate the cAMP and MAPK/ERK signaling pathway, albeit with different potency orders.
  2. 02LabIn vitro (cells)Activity measurement

    In cells expressing cavefish Mc3r (omMc3r), NDP-MSH activated the MAPK/ERK signaling pathway.

    In plain wordsIn cells carrying the cavefish Mc3r receptor, NDP-MSH switched on the MAPK/ERK signaling pathway.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseMAPK/ERK signaling activation via omMc3r
    Study

    Onychostoma macrolepisn: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The four agonists α-MSH, β-MSH, NDP-MSH, and ACTH (1-24) can all activate the cAMP and MAPK/ERK signaling pathway, albeit with different potency orders.
  3. 03LabIn vitro (cells)Activity measurement

    In cells expressing cavefish Mc3r, NDP-MSH had the highest activation effect on the MAPK/ERK pathway among the agonists tested.

    In plain wordsIn cells carrying the cavefish Mc3r receptor, NDP-MSH activated the MAPK/ERK pathway more strongly than the other agonists tested.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseMAPK/ERK activation compared with other agonists
    Study

    Onychostoma macrolepisn: not reported in source

    Comparator
    alpha-MSH, beta-MSH and ACTH (1-24)
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The "primitive" ligand ACTH (1-24) had the highest potency on the cAMP signaling pathway, while the synthetic ligand NDP-MSH had the highest activation effect on the MAPK/ERK signaling pathway.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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