The protective effects of S14G-humanin (HNG) against mono-sodium urate (MSU) crystals- induced gouty arthritis.

Zhang J, Lei H, Li X

Bioengineered1 Jan 2022Cited by 9 (Europe PMC)

PMID34965184PMCIDPMC8805931DOI10.1080/21655979.2021.2001911PepBankPUB-34965184

Summary

Gout is a common and complex form of arthritis that has brought great inconveniences to the normal lives of patients. It is reported that oxidative stress and nod-like receptor family protein 3 (NLRP3) inflammasome-mediated inflammatory reactions are involved in the pathogenesis of gout arthritis. S14G-humanin (S14G-HNG) is a modified peptide of HNG with higher inhibitory activity on the accumulation and deposition of Aβ. Recently, S14G-HNG has been reported to exert great anti-inflammatory effects. The present study proposed to explore the possible therapeutic property of S14G-HNG against gout arthritis. An animal model was established by stimulation with mono-sodium urate (MSU) crystals, followed by treatment with colchicine and S14G-HNG, respectively. The elevated Gait score promoted synovitis score and activated myeloperoxidase (MPO) observed in MSU crystals-treated mice were significantly reversed by colchicine and S14G-HNG. Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG. The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1). Lastly, the protective effects of S14G-HNG against MSU crystals-induced NLRP3 inflammasome activation were significantly abolished by the knockdown of SIRT1. In conclusion, our data reveal that S14G-HNG could possess potential benefits against MSU crystals-induced gout arthritis, with colchicine displaying a better effect.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone10

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

01AnimalNot a test of Humanin aloneAnimal study (in vivo)Efficacy outcome
In mice with MSU crystal-induced gout arthritis, S14G-HNG reversed the elevated gait score.
In plain wordsIn mice with gout-like arthritis, a humanin analog reversed the rise in gait score.
Peptide
Humanin
Outcome
DecreaseGait score
Study
mousen: not reported in source
MSU crystal-induced gouty arthritis
Comparator
MSU crystal-treated mice
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The elevated Gait score promoted synovitis score and activated myeloperoxidase (MPO) observed in MSU crystals-treated mice were significantly reversed by colchicine and S14G-HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
An animal model was established by stimulation with mono-sodium urate (MSU) crystals, followed by treatment with colchicine and S14G-HNG, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518401
02AnimalNot a test of Humanin aloneAnimal study (in vivo)Efficacy outcome
In mice with MSU crystal-induced gout arthritis, S14G-HNG reversed the elevated synovitis score.
In plain wordsIn mice with gout-like arthritis, a humanin analog reversed the rise in joint lining inflammation score.
Peptide
Humanin
Outcome
DecreaseSynovitis score
Study
mousen: not reported in source
MSU crystal-induced gouty arthritis
Comparator
MSU crystal-treated mice
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The elevated Gait score promoted synovitis score and activated myeloperoxidase (MPO) observed in MSU crystals-treated mice were significantly reversed by colchicine and S14G-HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
An animal model was established by stimulation with mono-sodium urate (MSU) crystals, followed by treatment with colchicine and S14G-HNG, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518402
03AnimalNot a test of Humanin aloneAnimal study (in vivo)Efficacy outcome
In mice with MSU crystal-induced gout arthritis, S14G-HNG reversed the activated myeloperoxidase.
In plain wordsIn mice with gout-like arthritis, a humanin analog reversed the rise in an inflammation enzyme.
Peptide
Humanin
Outcome
DecreaseMyeloperoxidase (MPO) activity
Study
mousen: not reported in source
MSU crystal-induced gouty arthritis
Comparator
MSU crystal-treated mice
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
The elevated Gait score promoted synovitis score and activated myeloperoxidase (MPO) observed in MSU crystals-treated mice were significantly reversed by colchicine and S14G-HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
An animal model was established by stimulation with mono-sodium urate (MSU) crystals, followed by treatment with colchicine and S14G-HNG, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518403
04LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages stimulated with MSU crystals, S14G-HNG at 25 and 50 μM reversed the increased mitochondrial reactive oxygen species.
In plain wordsIn mouse immune cells in lab tests, a humanin analog reversed a rise in mitochondrial oxidative stress.
Peptide
Humanin
Outcome
DecreaseMitochondrial ROS in macrophages
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Comparator
MSU crystals without S14G-HNG
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1).
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518404
05LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages stimulated with MSU crystals, S14G-HNG at 25 and 50 μM reversed the increased malondialdehyde levels.
In plain wordsIn mouse immune cells in lab tests, a humanin analog reversed a rise in an oxidative stress marker.
Peptide
Humanin
Outcome
DecreaseMalondialdehyde (MDA) in macrophages
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Comparator
MSU crystals without S14G-HNG
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1).
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518405
06LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages stimulated with MSU crystals, S14G-HNG at 25 and 50 μM reversed the upregulated NADPH oxidase-4.
In plain wordsIn mouse immune cells in lab tests, a humanin analog reversed a rise in the enzyme NOX-4.
Peptide
Humanin
Outcome
DecreaseNOX-4 expression in macrophages
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Comparator
MSU crystals without S14G-HNG
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1).
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518406
07LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages stimulated with MSU crystals, S14G-HNG at 25 and 50 μM reversed activation of the NLRP3 inflammasome.
In plain wordsIn mouse immune cells in lab tests, a humanin analog reversed activation of an inflammation complex.
Peptide
Humanin
Outcome
DecreaseNLRP3 inflammasome activation in macrophages
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Comparator
MSU crystals without S14G-HNG
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1).
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518407
08LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages stimulated with MSU crystals, S14G-HNG at 25 and 50 μM reversed the elevated production of inflammatory factors.
In plain wordsIn mouse immune cells in lab tests, a humanin analog reversed a rise in inflammation signals.
Peptide
Humanin
Outcome
DecreaseInflammatory factor production in macrophages
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Comparator
MSU crystals without S14G-HNG
Outcome group
Inflammation markers · Musculoskeletal
Source sentences
Summary
The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1).
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518408
09LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages stimulated with MSU crystals, S14G-HNG at 25 and 50 μM was accompanied by upregulation of sirtuin type-1.
In plain wordsIn mouse immune cells in lab tests, a humanin analog came with a rise in the protein SIRT1.
Peptide
Humanin
Outcome
IncreaseSIRT1 expression in macrophages
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Comparator
MSU crystals without S14G-HNG
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
The increased mitochondrial reactive oxygen species (ROS) and Malondialdehyde (MDA) levels, upregulated NADPH oxidase-4 (NOX-4), activated NLRP3 inflammasome, and elevated production of inflammatory factors in MSU crystals-treated BMDMs were dramatically reversed by S14G-HNG, accompanied by the upregulation of sirtuin type-1 (SIRT1).
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518409
10LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In mouse macrophages, SIRT1 knockdown abolished the protective effects of S14G-HNG against MSU crystal-induced NLRP3 inflammasome activation.
In plain wordsIn mouse immune cells in lab tests, removing SIRT1 cancelled the protective effect of a humanin analog.
Peptide
Humanin
Outcome
Not applicableEffect of SIRT1 knockdown on S14G-HNG protection against NLRP3 activation
Study
mousen: not reported in source
Bone marrow-derived macrophages stimulated with MSU crystals
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
Lastly, the protective effects of S14G-HNG against MSU crystals-induced NLRP3 inflammasome activation were significantly abolished by the knockdown of SIRT1.
1 context sentence(design, population, supporting numbers)
Summary · context
Bone marrow-derived macrophages (BMDMs) were isolated from mice and stimulated with MSU crystals, followed by being treated with 25 and 50 μM S14G-HNG.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Show in the study text Report a problemFND-3496518410

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

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