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Study · Animal study (in vivo) · mouse · 2021

4 results

Adjunctive Thymosin Beta-4 Treatment Influences PMN Effector Cell Function during Pseudomonas aeruginosa-Induced Corneal Infection.

Wang Y, Carion TW, Ebrahim AS, Sosne G, Berger EA

Cells18 Dec 2021Cited by 12 (Europe PMC)

PMID34944086 (opens in a new tab)PMCIDPMC8700181 (opens in a new tab)DOI10.3390/cells10123579 (opens in a new tab)PepBankPUB-34944086
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Summary

Previous work examining the therapeutic efficacy of adjunct thymosin beta 4 (Tβ4) to ciprofloxacin for ocular infectious disease has revealed markedly reduced inflammation (inflammatory mediators and innate immune cells) with increased activation of wound healing pathways. Understanding the therapeutic mechanisms of action have further revealed a synergistic effect with ciprofloxacin to enhance bacterial killing along with a regulatory influence over macrophage effector cell function. As a natural extension of the aforementioned work, the current study uses an experimental model of P. aeruginosa-induced keratitis to examine the influence of Tβ4 regarding polymorphonuclear leukocyte (PMN/neutrophil) cellular function, contributing to improved disease response. Flow cytometry was utilized to phenotypically profile infiltrating PMNs after infection. The generation of reactive oxygen species (ROS), neutrophil extracellular traps (NETs), and PMN apoptosis were investigated to assess the functional activities of PMNs in response to Tβ4 therapy. In vitro work using peritoneal-derived PMNs was similarly carried out to verify and extend our in vivo findings. The results indicate that the numbers of infiltrated PMNs into infected corneas were significantly reduced with adjunctive Tβ4 treatment. This was paired with the downregulated expression of proinflammatory markers on these cells, as well. Data generated from PMN functional studies suggested that the corneas of adjunctive Tβ4 treated B6 mice exhibit a well-regulated production of ROS, NETs, and limited PMN apoptosis. In addition to confirming the in vivo results, the in vitro findings also demonstrated that neutrophil elastase (NE) was unnecessary for NETosis. Collectively, these data provide additional evidence that adjunctive Tβ4 + ciprofloxacin treatment is a promising option for bacterial keratitis that addresses both the infectious pathogen and cellular-mediated immune response, as revealed by the current study.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with P. aeruginosa keratitis, adjunctive Tβ4 treatment reduced the number of neutrophils that infiltrated infected corneas.

    In plain wordsIn mice with a corneal infection, adding Tβ4 to treatment reduced the number of neutrophils in the cornea.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseNeutrophil (PMN) infiltration into infected corneas
    Study

    mousen: not reported in source

    P. aeruginosa keratitis, B6 mice
    Outcome group
    Inflammation markers · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The results indicate that the numbers of infiltrated PMNs into infected corneas were significantly reduced with adjunctive Tβ4 treatment.
    1 context sentence
    Summary · context
    As a natural extension of the aforementioned work, the current study uses an experimental model of P. aeruginosa-induced keratitis to examine the influence of Tβ4 regarding polymorphonuclear leukocyte (PMN/neutrophil) cellular function, contributing to improved disease response.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In mice with P. aeruginosa keratitis, adjunctive Tβ4 treatment lowered expression of proinflammatory markers on infiltrating neutrophils.

    In plain wordsIn mice with a corneal infection, adding Tβ4 lowered inflammatory markers on neutrophils.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseProinflammatory marker expression on neutrophils
    Study

    mousen: not reported in source

    P. aeruginosa keratitis, B6 mice
    Outcome group
    Inflammation markers · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    This was paired with the downregulated expression of proinflammatory markers on these cells, as well.
    1 context sentence
    Summary · context
    As a natural extension of the aforementioned work, the current study uses an experimental model of P. aeruginosa-induced keratitis to examine the influence of Tβ4 regarding polymorphonuclear leukocyte (PMN/neutrophil) cellular function, contributing to improved disease response.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In mice with P. aeruginosa keratitis, corneas treated with adjunctive Tβ4 showed well-regulated production of reactive oxygen species and neutrophil extracellular traps.

    In plain wordsIn mice with a corneal infection, corneas treated with adjunctive Tβ4 showed well-regulated neutrophil activity.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Direction not reportedNeutrophil ROS and NET production
    Study

    mousen: not reported in source

    P. aeruginosa keratitis, B6 mice
    Outcome group
    Immune response · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Data generated from PMN functional studies suggested that the corneas of adjunctive Tβ4 treated B6 mice exhibit a well-regulated production of ROS, NETs, and limited PMN apoptosis.
    1 context sentence
    Summary · context
    As a natural extension of the aforementioned work, the current study uses an experimental model of P. aeruginosa-induced keratitis to examine the influence of Tβ4 regarding polymorphonuclear leukocyte (PMN/neutrophil) cellular function, contributing to improved disease response.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In mice with P. aeruginosa keratitis, corneas treated with adjunctive Tβ4 showed limited neutrophil apoptosis.

    In plain wordsIn mice with a corneal infection, corneas treated with adjunctive Tβ4 showed limited neutrophil cell death.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Direction not reportedNeutrophil apoptosis
    Study

    mousen: not reported in source

    P. aeruginosa keratitis, B6 mice
    Outcome group
    Immune response · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Data generated from PMN functional studies suggested that the corneas of adjunctive Tβ4 treated B6 mice exhibit a well-regulated production of ROS, NETs, and limited PMN apoptosis.
    1 context sentence
    Summary · context
    As a natural extension of the aforementioned work, the current study uses an experimental model of P. aeruginosa-induced keratitis to examine the influence of Tβ4 regarding polymorphonuclear leukocyte (PMN/neutrophil) cellular function, contributing to improved disease response.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

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