Development and validation of SARMs and metabolic modulators screening in hair using UHPLC-MS/MS: Application to a doping case and first identification of S23 in authentic human hair.

Gheddar L, Raul JS, Kintz P

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences17 Nov 2021Cited by 4 (Europe PMC)

PMID34814052DOI10.1016/j.jchromb.2021.123048PepBankPUB-34814052

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MK-677 (ibutamoren).

01LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, the assay showed linearity for all compounds, including MK‑677, over the reported concentration ranges.
In plain wordsIn lab tests, the hair assay showed linearity for all tested compounds, including MK‑677.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableAssay linearity
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Linearity was verified for all compounds between 0.5 and 50 and 2000 pg/mg.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405201
02LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, detection limits across analytes including MK‑677 ranged from 0.1-20 pg/mg.
In plain wordsIn lab tests, detection limits across compounds including MK‑677 ranged from 0.1-20 pg/mg.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableDetection limit
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
LOD and LOQ were determined between 0.1-20 and 0.5-50 pg/mg respectively, according to the various analytes.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405202
03LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, quantification limits across analytes including MK‑677 ranged from 0.5-50 pg/mg.
In plain wordsIn lab tests, quantification limits across compounds including MK‑677 ranged from 0.5-50 pg/mg.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableQuantification limit
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
LOD and LOQ were determined between 0.1-20 and 0.5-50 pg/mg respectively, according to the various analytes.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405203
04LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, intra-day precision across compounds including MK‑677 had CV < 20%.
In plain wordsIn lab tests, within-day assay variation across compounds including MK‑677 was below 20%.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableIntra-day precision
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Intra- and inter-day precision (CV < 20%), matrix effects and recovery were evaluated for all compounds with CVs < 20%.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405204
05LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, inter-day precision across compounds including MK‑677 had CV < 20%.
In plain wordsIn lab tests, between-day assay variation across compounds including MK‑677 was below 20%.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableInter-day precision
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Intra- and inter-day precision (CV < 20%), matrix effects and recovery were evaluated for all compounds with CVs < 20%.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405205
06LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, matrix effect evaluations across compounds including MK‑677 had CVs < 20%.
In plain wordsIn lab tests, matrix effect evaluations across compounds including MK‑677 had variation below 20%.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableMatrix effects
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Intra- and inter-day precision (CV < 20%), matrix effects and recovery were evaluated for all compounds with CVs < 20%.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405206
07LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In hair analysis, recovery evaluations across compounds including MK‑677 had CVs < 20%.
In plain wordsIn lab tests, recovery evaluations across compounds including MK‑677 had variation below 20%.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableRecovery
Study
n: not reported in source
hair analysis
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Intra- and inter-day precision (CV < 20%), matrix effects and recovery were evaluated for all compounds with CVs < 20%.
1 context sentence(design, population, supporting numbers)
Summary · context
A method has been developed and validated for the analysis in hair of 9 SARMs (AC262536, ACP-105, andarine, LGD-4033, MK-0773, MK 677, ostarine, RAD 410 and S23) and 2 other metabolic modulators (GW501516, SR9009), using liquid chromatography coupled to tandem mass spectrometry.
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-3481405207

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

Keywords

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