The protective effects of S14G-humanin (HNG) against lipopolysaccharide (LPS)- induced inflammatory response in human dental pulp cells (hDPCs) mediated by the TLR4/MyD88/NF-κB pathway.

Zhang P, Cui Z, Li S

Bioengineered1 Dec 2021Cited by 16 (Europe PMC)

PMID34605740PMCIDPMC8806744DOI10.1080/21655979.2021.1979914PepBankPUB-34605740

Summary

Pulpitis is reported in large populations of patients and significantly impacts their normal life quality. It is reported that the lipopolysaccharide (LPS) in Gram-negative bacteria induces severe inflammation in dental pulp tissues. S14G-humanin is a derivative of humanin and has been recently confirmed to possess promising anti-inflammatory properties. The current study aims to explore the possibility of treating pulpitis with S14G-humanin. LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis. We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin. In addition, the LPS-induced elevated expression levels of toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (Myd88), and activation of the IκBα/NF-κB signaling pathway in hDPCs were significantly repressed by treatment with S14G-humanin. Conclusively, we found that S14G-humanin protected LPS-treated hDPCs by inhibiting the TLR4/MyD88/NF-κB signaling pathway.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the elevated expression and production of IL-6.
In plain wordsIn cells, S14G-humanin reversed the rise in IL-6 caused by LPS.
Peptide
Humanin
Outcome
DecreaseIL-6 expression and production
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574001
02LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the elevated expression and production of TNF-α.
In plain wordsIn cells, S14G-humanin reversed the rise in TNF-α caused by LPS.
Peptide
Humanin
Outcome
DecreaseTNF-α expression and production
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574002
03LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the elevated expression and production of MCP-1.
In plain wordsIn cells, S14G-humanin reversed the rise in MCP-1 caused by LPS.
Peptide
Humanin
Outcome
DecreaseMCP-1 expression and production
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574003
04LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the elevated expression and production of MMP-2.
In plain wordsIn cells, S14G-humanin reversed the rise in MMP-2 caused by LPS.
Peptide
Humanin
Outcome
DecreaseMMP-2 expression and production
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574004
05LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the elevated expression and production of MMP-9.
In plain wordsIn cells, S14G-humanin reversed the rise in MMP-9 caused by LPS.
Peptide
Humanin
Outcome
DecreaseMMP-9 expression and production
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574005
06LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the upregulation of PTX3.
In plain wordsIn cells, S14G-humanin reversed the rise in PTX3 caused by LPS.
Peptide
Humanin
Outcome
DecreasePentraxin 3 (PTX3) expression
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574006
07LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin at 50 and 100 μM reversed the activated oxidative stress.
In plain wordsIn cells, S14G-humanin reversed the oxidative stress caused by LPS.
Peptide
Humanin
Outcome
DecreaseOxidative stress
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 μM S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574007
08LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin significantly repressed the LPS-induced elevated expression of TLR4.
In plain wordsIn cells, S14G-humanin lowered the LPS-driven rise of the receptor TLR4.
Peptide
Humanin
Outcome
DecreaseTLR4 expression
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
In addition, the LPS-induced elevated expression levels of toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (Myd88), and activation of the IκBα/NF-κB signaling pathway in hDPCs were significantly repressed by treatment with S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574008
09LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin significantly repressed the LPS-induced elevated expression of MyD88.
In plain wordsIn cells, S14G-humanin lowered the LPS-driven rise of the signal protein MyD88.
Peptide
Humanin
Outcome
DecreaseMyD88 expression
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
In addition, the LPS-induced elevated expression levels of toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (Myd88), and activation of the IκBα/NF-κB signaling pathway in hDPCs were significantly repressed by treatment with S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574009
10LabIn vitro (cells)Mechanism
In LPS-treated human dental pulp cells, S14G-humanin significantly repressed LPS-induced activation of the IκBα/NF-κB signaling pathway.
In plain wordsIn cells, S14G-humanin reduced activation of the IκBα/NF-κB inflammation pathway caused by LPS.
Peptide
Humanin
Outcome
DecreaseIκBα/NF-κB pathway activation
Study
humann: not reported in source
Human dental pulp cells (hDPCs) stimulated with LPS
Comparator
LPS-treated cells without S14G-humanin
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
50 and 100 μMAs given in this study (human).
Source sentences
Summary
In addition, the LPS-induced elevated expression levels of toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (Myd88), and activation of the IκBα/NF-κB signaling pathway in hDPCs were significantly repressed by treatment with S14G-humanin.
1 context sentence(design, population, supporting numbers)
Summary · context
LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis.
Show in the study text Report a problemFND-3460574010

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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