Thymosin β4 Suppresses LPS-Induced Murine Lung Fibrosis by Attenuating Oxidative Injury and Alleviating Inflammation.

Tian Z, Yao N, Wang F, Ruan L

Inflammation19 Aug 2021Cited by 21 (Europe PMC)

PMID34414534DOI10.1007/s10753-021-01528-6PepBankPUB-34414534

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

5 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In HPAEpiC cells, Tβ4 significantly attenuated the LPS-induced inhibition of mitophagy.
In plain wordsIn cells, Tβ4 reduced the block of mitophagy caused by LPS.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseLPS-induced mitophagy inhibition
Study
humann: not reported in source
HPAEpiC cells with LPS
Outcome group
Mitochondrial function · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Our in vitro experiments also showed that LPS inhibited mitophagy and promoted inflammation via oxidative stress in HPAEpiC, and Tβ4 significantly attenuated LPS-induced mitophagy inhibition, inflammasome activation, and transforming growth factor-β (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in HPAEpiC.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro tests with HPAEpiC and HLF-1 cells were performed to confirm the effects of Tβ4.
Report a problemFND-3441453406
02LabIn vitro (cells)Mechanism
In HPAEpiC cells, Tβ4 significantly attenuated LPS-induced inflammasome activation.
In plain wordsIn cells, Tβ4 reduced inflammasome activation caused by LPS.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseLPS-induced inflammasome activation
Study
humann: not reported in source
HPAEpiC cells with LPS
Outcome group
Inflammation markers · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Our in vitro experiments also showed that LPS inhibited mitophagy and promoted inflammation via oxidative stress in HPAEpiC, and Tβ4 significantly attenuated LPS-induced mitophagy inhibition, inflammasome activation, and transforming growth factor-β (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in HPAEpiC.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro tests with HPAEpiC and HLF-1 cells were performed to confirm the effects of Tβ4.
Report a problemFND-3441453407
03LabIn vitro (cells)Mechanism
In HPAEpiC cells, Tβ4 significantly attenuated TGF-β1-induced epithelial-mesenchymal transition.
In plain wordsIn cells, Tβ4 reduced the TGF-β1-induced change of lung cells toward a fibrotic type.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseTGF-β1-induced epithelial-mesenchymal transition
Study
humann: not reported in source
HPAEpiC cells with TGF-β1
Outcome group
Lung injury and fibrosis · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Our in vitro experiments also showed that LPS inhibited mitophagy and promoted inflammation via oxidative stress in HPAEpiC, and Tβ4 significantly attenuated LPS-induced mitophagy inhibition, inflammasome activation, and transforming growth factor-β (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in HPAEpiC.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro tests with HPAEpiC and HLF-1 cells were performed to confirm the effects of Tβ4.
Report a problemFND-3441453408
04LabIn vitro (cells)Mechanism
Tβ4 suppressed the proliferation of HLF-1 cells.
In plain wordsIn cells, Tβ4 slowed the growth of lung fibroblast cells.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseHLF-1 cell proliferation
Study
humann: not reported in source
HLF-1 cells
Outcome group
Lung injury and fibrosis · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Moreover, Tβ4 suppressed the proliferation and attenuated the TGF-β1-induced activation of HLF-1 cells.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro tests with HPAEpiC and HLF-1 cells were performed to confirm the effects of Tβ4.
Report a problemFND-3441453409
05LabIn vitro (cells)Mechanism
Tβ4 attenuated the TGF-β1-induced activation of HLF-1 cells.
In plain wordsIn cells, Tβ4 reduced the TGF-β1-induced activation of lung fibroblast cells.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseTGF-β1-induced activation of HLF-1 cells
Study
humann: not reported in source
HLF-1 cells with TGF-β1
Outcome group
Lung injury and fibrosis · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Moreover, Tβ4 suppressed the proliferation and attenuated the TGF-β1-induced activation of HLF-1 cells.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro tests with HPAEpiC and HLF-1 cells were performed to confirm the effects of Tβ4.
Report a problemFND-3441453410

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

06HumanEndogenous levelObservational studyOther
Tβ4 expression was markedly higher in human or mouse fibrotic lung tissues.
In plain wordsThe body's own Tβ4 was much higher in fibrotic lung tissue of people or mice.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseTβ4 expression in fibrotic lung tissue
Study
human or mousen: not reported in source
Outcome group
Lung injury and fibrosis · Respiratory
Source sentences
Summary
Tβ4 was markedly upregulated in human or mouse fibrotic lung tissues.
1 context sentence(design, population, supporting numbers)
Summary · context
Expression of Tβ4 in fibrotic lung tissues was assessed by real-time quantitative reverse-transcription PCR (rt-PCR), immunohistochemistry (IHC), and western blotting.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3441453401

Studies that did not test the peptide alone4

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

07AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In mice with LPS-induced lung injury, AAV-Tβ4 markedly reduced oxidative damage.
In plain wordsIn mice with lung injury, a virus that raises Tβ4 gene activity strongly reduced oxidative damage.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseLPS-induced oxidative damage
Study
mousen: not reported in source
LPS-induced lung injury and fibrosis
Outcome group
Oxidative stress · Respiratory
Source sentences
Summary
AAV-Tβ4 markedly alleviated LPS-induced oxidative damage, lung injury, inflammation, and fibrosis in mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of intraperitoneal adeno-associated virus-Tβ4 (AAV-Tβ4) on LPS-induced lung injury and fibrosis were observed through the evaluation of collagen deposition and α-smooth muscle actin (SMA) expression.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3441453402
08AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In mice with LPS-induced lung injury, AAV-Tβ4 markedly reduced lung injury.
In plain wordsIn mice with lung injury, a virus that raises Tβ4 gene activity strongly reduced lung injury.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseLPS-induced lung injury
Study
mousen: not reported in source
LPS-induced lung injury and fibrosis
Outcome group
Lung injury and fibrosis · Respiratory
Source sentences
Summary
AAV-Tβ4 markedly alleviated LPS-induced oxidative damage, lung injury, inflammation, and fibrosis in mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of intraperitoneal adeno-associated virus-Tβ4 (AAV-Tβ4) on LPS-induced lung injury and fibrosis were observed through the evaluation of collagen deposition and α-smooth muscle actin (SMA) expression.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3441453403
09AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In mice with LPS-induced lung injury, AAV-Tβ4 markedly reduced inflammation.
In plain wordsIn mice with lung injury, a virus that raises Tβ4 gene activity strongly reduced inflammation.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseLPS-induced lung inflammation
Study
mousen: not reported in source
LPS-induced lung injury and fibrosis
Outcome group
Inflammation markers · Respiratory
Source sentences
Summary
AAV-Tβ4 markedly alleviated LPS-induced oxidative damage, lung injury, inflammation, and fibrosis in mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of intraperitoneal adeno-associated virus-Tβ4 (AAV-Tβ4) on LPS-induced lung injury and fibrosis were observed through the evaluation of collagen deposition and α-smooth muscle actin (SMA) expression.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3441453404
10AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In mice with LPS-induced lung injury, AAV-Tβ4 markedly reduced fibrosis.
In plain wordsIn mice with lung injury, a virus that raises Tβ4 gene activity strongly reduced scarring (fibrosis).
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseLPS-induced lung fibrosis
Study
mousen: not reported in source
LPS-induced lung injury and fibrosis
Outcome group
Lung injury and fibrosis · Respiratory
Source sentences
Summary
AAV-Tβ4 markedly alleviated LPS-induced oxidative damage, lung injury, inflammation, and fibrosis in mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of intraperitoneal adeno-associated virus-Tβ4 (AAV-Tβ4) on LPS-induced lung injury and fibrosis were observed through the evaluation of collagen deposition and α-smooth muscle actin (SMA) expression.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3441453405

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

Keywords

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