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Study · Randomized controlled trial · n = 48 · 2021

12 results

Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study.

Liao B, Zhao Y, Wang D, Zhang X, Hao X, Hu M

Journal of the International Society of Sports Nutrition8 Jul 2021Cited by 75 (Europe PMC)

PMID34238308 (opens in a new tab)PMCIDPMC8265078 (opens in a new tab)DOI10.1186/s12970-021-00442-4 (opens in a new tab)PepBankPUB-34238308
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Summary

BackgroundRecent studies in rodents indicate that a combination of exercise training and supplementation with nicotinamide adenine dinucleotide (NAD+) precursors has synergistic effects. However, there are currently no human clinical trials analyzing this.ObjectiveThis study investigates the effects of a combination of exercise training and supplementation with nicotinamide mononucleotide (NMN), the immediate precursor of NAD+, on cardiovascular fitness in healthy amateur runners.MethodsA six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted. The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo). Each group consisted of ten male participants and two female participants. Each training session was 40-60 min, and the runners trained 5-6 times each week. Cardiopulmonary exercise testing was performed at baseline and after the intervention, at 6 weeks, to assess the aerobic capacity of the runners.ResultsAnalysis of covariance of the change from baseline over the 6 week treatment showed that the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group. However, there was no difference in VO2max, O2-pulse, VO2 related to work rate, and peak power after the 6 week treatment from baseline in any of these groups.ConclusionNMN increases the aerobic capacity of humans during exercise training, and the improvement is likely the result of enhanced O2 utilization of the skeletal muscle.Trial registration numberChiCTR2000035138 .
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study12

12 results from studies that gave the peptide. 12 results from people.

  1. 01HumanRandomized controlled trialEfficacy outcome

    In amateur runners, maximum oxygen uptake did not change from baseline after 6 weeks in the NMN groups (300, 600 or 1200 mg/day) or the placebo group.

    In plain wordsIn amateur runners, maximum oxygen uptake did not change from baseline after 6 weeks in any NMN dose group or the placebo group.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeMaximum oxygen uptake
    Study

    n = 48

    Comparator
    Baseline
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    300, 600 and 1200 mg/day in three NMN groups · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    However, there was no difference in VO2max, O2-pulse, VO2 related to work rate, and peak power after the 6 week treatment from baseline in any of these groups.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  2. 02HumanRandomized controlled trialEfficacy outcome

    In amateur runners, oxygen pulse did not change from baseline after 6 weeks in the NMN groups (300, 600 or 1200 mg/day) or the placebo group.

    In plain wordsIn amateur runners, oxygen pulse did not change from baseline after 6 weeks in any NMN dose group or the placebo group.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeOxygen pulse
    Study

    n = 48

    Comparator
    Baseline
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    300, 600 and 1200 mg/day in three NMN groups · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    However, there was no difference in VO2max, O2-pulse, VO2 related to work rate, and peak power after the 6 week treatment from baseline in any of these groups.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  3. 03HumanRandomized controlled trialEfficacy outcome

    In amateur runners, oxygen uptake related to work rate did not change from baseline after 6 weeks in the NMN groups (300, 600 or 1200 mg/day) or the placebo group.

    In plain wordsIn amateur runners, oxygen uptake relative to work rate did not change from baseline in the NMN dose groups or placebo group.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeOxygen uptake related to work rate
    Study

    n = 48

    Comparator
    Baseline
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    300, 600 and 1200 mg/day in three NMN groups · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    However, there was no difference in VO2max, O2-pulse, VO2 related to work rate, and peak power after the 6 week treatment from baseline in any of these groups.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  4. 04HumanRandomized controlled trialEfficacy outcome

    In amateur runners, peak power did not change from baseline after 6 weeks in the NMN groups (300, 600 or 1200 mg/day) or the placebo group.

    In plain wordsIn amateur runners, peak power did not change from baseline after 6 weeks in any NMN dose group or the placebo group.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changePeak power
    Study

    n = 48

    Comparator
    Baseline
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    300, 600 and 1200 mg/day in three NMN groups · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    However, there was no difference in VO2max, O2-pulse, VO2 related to work rate, and peak power after the 6 week treatment from baseline in any of these groups.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  5. 05HumanRandomized controlled trialEfficacy outcome

    In amateur runners, NMN at the medium dose increased oxygen uptake more than placebo during exercise training.

    In plain wordsIn amateur runners, medium-dose NMN increased oxygen uptake more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseOxygen uptake
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    600 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  6. 06HumanRandomized controlled trialEfficacy outcome

    In amateur runners, NMN at the high dose increased oxygen uptake more than placebo during exercise training.

    In plain wordsIn amateur runners, high-dose NMN increased oxygen uptake more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseOxygen uptake
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    1200 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  7. 07HumanRandomized controlled trialEfficacy outcome

    In amateur runners, medium-dose NMN increased percentage of maximum oxygen uptake more than placebo during exercise training.

    In plain wordsIn amateur runners, medium-dose NMN increased percentage of maximum oxygen uptake more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreasePercentage of maximum oxygen uptake
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    600 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  8. 08HumanRandomized controlled trialEfficacy outcome

    In amateur runners, high-dose NMN increased percentage of maximum oxygen uptake more than placebo during exercise training.

    In plain wordsIn amateur runners, high-dose NMN increased percentage of maximum oxygen uptake more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreasePercentage of maximum oxygen uptake
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    1200 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  9. 09HumanRandomized controlled trialEfficacy outcome

    In amateur runners, medium-dose NMN increased power at the first ventilatory threshold more than placebo during exercise training.

    In plain wordsIn amateur runners, medium-dose NMN increased power at the first ventilatory threshold more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreasePower at first ventilatory threshold
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    600 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  10. 10HumanRandomized controlled trialEfficacy outcome

    In amateur runners, high-dose NMN increased power at the first ventilatory threshold more than placebo during exercise training.

    In plain wordsIn amateur runners, high-dose NMN increased power at the first ventilatory threshold more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreasePower at first ventilatory threshold
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    1200 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  11. 11HumanRandomized controlled trialEfficacy outcome

    In amateur runners, medium-dose NMN increased power at the second ventilatory threshold more than placebo during exercise training.

    In plain wordsIn amateur runners, medium-dose NMN increased power at the second ventilatory threshold more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreasePower at second ventilatory threshold
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    600 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).
  12. 12HumanRandomized controlled trialEfficacy outcome

    In amateur runners, high-dose NMN increased power at the second ventilatory threshold more than placebo during exercise training.

    In plain wordsIn amateur runners, high-dose NMN increased power at the second ventilatory threshold more than placebo during exercise training.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreasePower at second ventilatory threshold
    Study

    n: not reported in source

    Comparator
    Placebo with exercise training
    Outcome group
    Exercise and physical function · Respiratory
    Studied dose
    1200 mg/day · 6 weeksAs given in this study (human).

    Source sentences

    Summary
    the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group.
    2 context sentences
    Summary · context
    A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted.
    Summary · context
    The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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