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Study · Animal study (in vivo) · zebrafish · 2021

2 results

The use of dipeptide supplementation as a means of mitigating the negative effects of dietary soybean meal on Zebrafish Danio rerio.

Molinari GS, Wojno M, McCracken VJ, Kwasek K

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology15 Apr 2021Cited by 10 (Europe PMC)

PMID33865992 (opens in a new tab)DOI10.1016/j.cbpa.2021.110958 (opens in a new tab)PepBankPUB-33865992
Publisher pagePubMedAsk about this study
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Summary

Soybean meal (SBM) inclusion in aquaculture diets has been found to negatively affect growth and induce intestinal inflammation in fish. The objective of this study was to determine the effect of health-promoting dipeptide supplementation into SBM-based feeds on growth performance, intestinal health, and muscle free amino acid composition, an indicator of dietary amino acid availability, in a zebrafish model. There were five treatment groups in this study. The first group ((+) Control) received a fishmeal-based diet. The second group ((-) Control) received SBM-based diet. The last three groups (Ala-Glu, Car, and Ans) were fed SBM-based diets, supplemented with alanyl-glutamine, carnosine, and anserine respectively. The Ala-Glu and Car groups experienced a significantly higher weight gain than the (-) Control group, weighing 35.38% and 33.96% more, respectively at the conclusion of the study. There were no significant differences in gene expression among the groups, but Ala-Glu had the highest expression of both nutrient absorption genes measured, PepT1 and fabp2. Ala-Glu had significantly longer intestinal villi, and a significantly higher villus length-to-width ratio than the (-) Control group. The Car group had a significantly higher post-prandial tissue concentration of lysine, compared to the (-) Control group. The increase in villus surface area and expression of nutrient absorption genes represent an improvement in intestinal absorptive capacity in the Ala-Glu group. The results from this study provide support for the use of alanyl-glutamine and carnosine supplementation as a means of improving growth performance of zebrafish fed with a high level SBM-based diet.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In zebrafish fed soybean meal-based diets, the carnosine group had significantly higher weight gain and weighed 33.96% more than controls.

    In plain wordsIn zebrafish fed soybean meal, carnosine increased weight gain compared with the unsupplemented diet.

    Peptide
    L-carnosine
    Outcome
    IncreaseWeight gain
    Study

    zebrafishn: not reported in source

    Zebrafish fed soybean meal-based diets
    Comparator
    Soybean meal-based diet without supplementation
    Outcome group
    Body weight · Metabolic and weight
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The Ala-Glu and Car groups experienced a significantly higher weight gain than the (-) Control group, weighing 35.38% and 33.96% more, respectively at the conclusion of the study.
    2 context sentences
    Summary · context
    The second group ((-) Control) received SBM-based diet.
    Summary · context
    The last three groups (Ala-Glu, Car, and Ans) were fed SBM-based diets, supplemented with alanyl-glutamine, carnosine, and anserine respectively.
  2. 02AnimalAnimal study (in vivo)Activity measurement

    In zebrafish fed soybean meal-based diets, carnosine significantly increased post-prandial tissue lysine concentration versus unsupplemented controls.

    In plain wordsIn zebrafish fed soybean meal, carnosine increased tissue lysine after feeding compared with the unsupplemented diet.

    Peptide
    L-carnosine
    Outcome
    IncreasePost-prandial tissue lysine concentration
    Study

    zebrafishn: not reported in source

    Zebrafish fed soybean meal-based diets
    Comparator
    Soybean meal-based diet without supplementation
    Outcome group
    Protein and amino acid metabolism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The Car group had a significantly higher post-prandial tissue concentration of lysine, compared to the (-) Control group.
    2 context sentences
    Summary · context
    The second group ((-) Control) received SBM-based diet.
    Summary · context
    The last three groups (Ala-Glu, Car, and Ans) were fed SBM-based diets, supplemented with alanyl-glutamine, carnosine, and anserine respectively.

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    27 Oct 2026, 18:20 UTC

    Study results updated

  2. Version 1

    27 Oct 2026, 16:32 UTC

    First added · source update from Europe PMC

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