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Study · Animal study (in vivo) · mouse · 2021

12 results

The IL-27 component EBI-3 and its receptor subunit IL-27Rα are essential for the cytoprotective action of humanin on male germ cells†.

Jia Y, Swerdloff RS, Lue Y, Dai-Ju J, Surampudi P, Cohen P, Wang C

Biology of reproduction1 Mar 2021Cited by 8 (Europe PMC)

PMID33330922 (opens in a new tab)PMCIDPMC8527998 (opens in a new tab)DOI10.1093/biolre/ioaa225 (opens in a new tab)PepBankPUB-33330922
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study12

11 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In wildtype mice with testicular hyperthermia, humanin inhibited heat-induced germ cell apoptosis.

    In plain wordsIn mice, humanin reduced germ cell death caused by heat in the testis.

    Peptide
    Humanin
    Outcome
    DecreaseHeat-induced germ cell apoptosis in wildtype mice
    Study

    Mousen: not reported in source

    Wildtype mice with testicular hyperthermia
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · intraperitonealAs given in this study (Mouse).

    Source sentences

    Summary
    We demonstrated that HN inhibited heat-induced germ cell apoptosis in wildtype
    1 context sentence
    Summary · context
    The cytoprotective action of HN was studied by using groups of il-27r-/- or ebi-3-/- mice administered the following treatment: (1) vehicle; (2) a single intraperitoneal (IP) injection of HN peptide; (3) testicular hyperthermia; and (4) testicular hyperthermia plus HN.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In il-27r-/- mice with testicular hyperthermia, humanin did not inhibit heat-induced germ cell apoptosis.

    In plain wordsIn mice that lack the IL-27 receptor, humanin did not reduce germ cell death caused by heat.

    Peptide
    Humanin
    Outcome
    No significant changeHeat-induced germ cell apoptosis in il-27r-/- mice
    Study

    Mousen: not reported in source

    il-27r-/- mice with testicular hyperthermia
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · intraperitonealAs given in this study (Mouse).

    Source sentences

    Summary
    We demonstrated that HN inhibited heat-induced germ cell apoptosis in wildtype but not in il-27r-/- or ebi-3-/- mice.
    1 context sentence
    Summary · context
    The cytoprotective action of HN was studied by using groups of il-27r-/- or ebi-3-/- mice administered the following treatment: (1) vehicle; (2) a single intraperitoneal (IP) injection of HN peptide; (3) testicular hyperthermia; and (4) testicular hyperthermia plus HN.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In ebi-3-/- mice with testicular hyperthermia, humanin did not inhibit heat-induced germ cell apoptosis.

    In plain wordsIn mice that lack EBI-3, humanin did not reduce germ cell death caused by heat.

    Peptide
    Humanin
    Outcome
    No significant changeHeat-induced germ cell apoptosis in ebi-3-/- mice
    Study

    Mousen: not reported in source

    ebi-3-/- mice with testicular hyperthermia
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · intraperitonealAs given in this study (Mouse).

    Source sentences

    Summary
    We demonstrated that HN inhibited heat-induced germ cell apoptosis in wildtype but not in il-27r-/- or ebi-3-/- mice.
    1 context sentence
    Summary · context
    The cytoprotective action of HN was studied by using groups of il-27r-/- or ebi-3-/- mice administered the following treatment: (1) vehicle; (2) a single intraperitoneal (IP) injection of HN peptide; (3) testicular hyperthermia; and (4) testicular hyperthermia plus HN.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In wildtype mice, humanin restored STAT3 phosphorylation that heat had suppressed.

    In plain wordsIn mice, humanin brought back a cell signal (STAT3) that heat had switched down.

    Peptide
    Humanin
    Outcome
    IncreaseSTAT3 phosphorylation in wildtype mice
    Study

    Mousen: not reported in source

    Wildtype mice with testicular hyperthermia
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · intraperitonealAs given in this study (Mouse).

    Source sentences

    Summary
    HN restored heat-suppressed STAT3 phosphorylation in wildtype
    1 context sentence
    Summary · context
    The cytoprotective action of HN was studied by using groups of il-27r-/- or ebi-3-/- mice administered the following treatment: (1) vehicle; (2) a single intraperitoneal (IP) injection of HN peptide; (3) testicular hyperthermia; and (4) testicular hyperthermia plus HN.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In il-27r-/- mice, humanin did not restore heat-suppressed STAT3 phosphorylation.

    In plain wordsIn mice that lack the IL-27 receptor, humanin did not bring back the heat-reduced STAT3 signal.

    Peptide
    Humanin
    Outcome
    No significant changeSTAT3 phosphorylation in il-27r-/- mice
    Study

    Mousen: not reported in source

    il-27r-/- mice with testicular hyperthermia
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · intraperitonealAs given in this study (Mouse).

    Source sentences

    Summary
    HN restored heat-suppressed STAT3 phosphorylation in wildtype but not il-27r-/- or ebi-3-/- mice.
    1 context sentence
    Summary · context
    The cytoprotective action of HN was studied by using groups of il-27r-/- or ebi-3-/- mice administered the following treatment: (1) vehicle; (2) a single intraperitoneal (IP) injection of HN peptide; (3) testicular hyperthermia; and (4) testicular hyperthermia plus HN.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In ebi-3-/- mice, humanin did not restore heat-suppressed STAT3 phosphorylation.

    In plain wordsIn mice that lack EBI-3, humanin did not bring back the heat-reduced STAT3 signal.

    Peptide
    Humanin
    Outcome
    No significant changeSTAT3 phosphorylation in ebi-3-/- mice
    Study

    Mousen: not reported in source

    ebi-3-/- mice with testicular hyperthermia
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · intraperitonealAs given in this study (Mouse).

    Source sentences

    Summary
    HN restored heat-suppressed STAT3 phosphorylation in wildtype but not il-27r-/- or ebi-3-/- mice.
    1 context sentence
    Summary · context
    The cytoprotective action of HN was studied by using groups of il-27r-/- or ebi-3-/- mice administered the following treatment: (1) vehicle; (2) a single intraperitoneal (IP) injection of HN peptide; (3) testicular hyperthermia; and (4) testicular hyperthermia plus HN.
  7. 07LabEx vivo tissueActivity measurement

    In ex vivo seminiferous tubule culture, humanin prevented heat-induced germ cell apoptosis.

    In plain wordsIn lab tests on testis tissue, humanin prevented germ cell death caused by heat.

    Peptide
    Humanin
    Outcome
    DecreaseHeat-induced germ cell apoptosis
    Study

    n: not reported in source

    Ex vivo seminiferous tubule culture
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We demonstrated in ex-vivo seminiferous tubules culture that HN prevented heat-induced germ cell apoptosis
  8. 08LabEx vivo tissueMechanism

    In ex vivo seminiferous tubule culture, anti-IL-27R, anti-gp130 and anti-EBI-3 antibodies blocked the protection by humanin against heat-induced germ cell apoptosis.

    In plain wordsIn lab tests on testis tissue, antibodies against three receptor parts blocked the protection that humanin gave against heat damage.

    Peptide
    Humanin
    Outcome
    DecreaseBlock of humanin protection by anti-IL-27R, anti-gp130 and anti-EBI-3 antibodies
    Study

    n: not reported in source

    Ex vivo seminiferous tubule culture
    Comparator
    No blocking antibody
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN prevented heat-induced germ cell apoptosis was blocked by specific anti-IL-27R, anti-gp130, and anti-EBI-3
  9. 09LabEx vivo tissueMechanism

    In ex vivo seminiferous tubule culture, anti-CNTFR antibodies did not significantly block the protection by humanin against heat-induced germ cell apoptosis.

    In plain wordsIn lab tests on testis tissue, an antibody against CNTFR did not block the protection that humanin gave against heat damage.

    Peptide
    Humanin
    Outcome
    No significant changeEffect of anti-CNTFR antibody on humanin protection
    Study

    n: not reported in source

    Ex vivo seminiferous tubule culture
    Comparator
    No blocking antibody
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We demonstrated in ex-vivo seminiferous tubules culture that HN prevented heat-induced germ cell apoptosis was blocked by specific anti-IL-27R, anti-gp130, and anti-EBI-3, but not by anti-CNTFR antibodies significantly.
  10. 10LabIn vitro (cell-free)Mechanism

    In dot blot analyses, humanin interacted directly with IL-27R.

    In plain wordsIn lab tests, humanin bound directly to the IL-27 receptor.

    Peptide
    Humanin
    Outcome
    Not applicableDirect interaction of humanin with IL-27R
    Study

    n: not reported in source

    Dot blot
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Dot blot analyses showed the direct interaction of HN with IL-27R or EBI-3 peptide.
  11. 11LabIn vitro (cell-free)Mechanism

    In dot blot analyses, humanin interacted directly with EBI-3 peptide.

    In plain wordsIn lab tests, humanin bound directly to the EBI-3 peptide.

    Peptide
    Humanin
    Outcome
    Not applicableDirect interaction of humanin with EBI-3
    Study

    n: not reported in source

    Dot blot
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Dot blot analyses showed the direct interaction of HN with IL-27R or EBI-3 peptide.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 12AnimalEndogenous levelAnimal study (in vivo)Mechanism

    Immunofluorescence staining showed IL-27R co-localized with humanin and gp130 in Leydig cells and germ cells.

    In plain wordsIn mouse testis, staining showed the IL-27 receptor in the same cells as the body's own humanin and gp130.

    Peptide
    Humanin
    Outcome
    Not applicableCo-localization of IL-27R with humanin and gp130 in testis
    Study

    Mousen: not reported in source

    Testis tissue
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health

    Source sentences

    Summary
    Immunofluorescence staining showed the co-localization of IL-27R with HN and gp130 in Leydig cells and germ cells.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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