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Study · Animal study (in vivo) · mouse · 2020

5 results

Adverse Neurological Effects of Short-Term Sleep Deprivation in Aging Mice Are Prevented by SS31 Peptide.

Wu J, Dou Y, Ladiges WC

Clocks & sleep6 Aug 2020Cited by 11 (Europe PMC)

PMID33089207 (opens in a new tab)PMCIDPMC7573804 (opens in a new tab)DOI10.3390/clockssleep2030024 (opens in a new tab)PepBankPUB-33089207
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Summary

Sleep deprivation is a potent stress factor that disrupts regulatory pathways in the brain resulting in cognitive dysfunction and increased risk of neurodegenerative disease with increasing age. Prevention of the adverse effects of sleep deprivation could be beneficial in older individuals by restoring healthy brain function. We report here on the ability of SS31, a mitochondrial specific peptide, to attenuate the negative neurological effects of short-term sleep deprivation in aging mice. C57BL/6 female mice, 20 months old, were subcutaneously injected with SS31 (3 mg/kg) or saline daily for four days. Sleep deprivation was 4 h daily for the last two days of SS31 treatment. Mice were immediately tested for learning ability followed by collection of brain and other tissues. In sleep deprived mice treated with SS31, learning impairment was prevented, brain mitochondrial ATP levels and synaptic plasticity regulatory proteins were restored, and reactive oxygen species (ROS) and inflammatory cytokines levels were decreased in the hippocampus. This observation suggests possible therapeutic benefits of SS31 for alleviating adverse neurological effects of short-term sleep loss.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In sleep-deprived aging mice, SS31 prevented learning impairment.

    In plain wordsIn sleep-deprived older mice, SS31 prevented learning problems.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseLearning impairment after sleep deprivation
    Study

    mousen: not reported in source

    Short-term sleep deprivation in aging C57BL/6 mice (20 months)
    Comparator
    Saline
    Outcome group
    Cognition and memory · Nervous system and cognition
    Studied dose
    3 mg/kg · subcutaneous · daily for four daysAs given in this study (mouse).

    Source sentences

    Summary
    In sleep deprived mice treated with SS31, learning impairment was prevented, brain mitochondrial ATP levels and synaptic plasticity regulatory proteins were restored, and reactive oxygen species (ROS) and inflammatory cytokines levels were decreased in the hippocampus.
    2 context sentences
    Summary · context
    C57BL/6 female mice, 20 months old, were subcutaneously injected with SS31 (3 mg/kg) or saline daily for four days.
    Summary · context
    Sleep deprivation was 4 h daily for the last two days of SS31 treatment.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In sleep-deprived aging mice, SS31 restored brain mitochondrial ATP levels.

    In plain wordsIn sleep-deprived older mice, SS31 restored energy (ATP) levels in brain mitochondria.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseBrain mitochondrial ATP levels
    Study

    mousen: not reported in source

    Short-term sleep deprivation in aging C57BL/6 mice (20 months)
    Comparator
    Saline
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    3 mg/kg · subcutaneous · daily for four daysAs given in this study (mouse).

    Source sentences

    Summary
    In sleep deprived mice treated with SS31, learning impairment was prevented, brain mitochondrial ATP levels and synaptic plasticity regulatory proteins were restored, and reactive oxygen species (ROS) and inflammatory cytokines levels were decreased in the hippocampus.
    2 context sentences
    Summary · context
    C57BL/6 female mice, 20 months old, were subcutaneously injected with SS31 (3 mg/kg) or saline daily for four days.
    Summary · context
    Sleep deprivation was 4 h daily for the last two days of SS31 treatment.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In sleep-deprived aging mice, SS31 restored synaptic plasticity regulatory proteins.

    In plain wordsIn sleep-deprived older mice, SS31 restored proteins that help brain connections adapt.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseSynaptic plasticity regulatory proteins
    Study

    mousen: not reported in source

    Short-term sleep deprivation in aging C57BL/6 mice (20 months)
    Comparator
    Saline
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    3 mg/kg · subcutaneous · daily for four daysAs given in this study (mouse).

    Source sentences

    Summary
    In sleep deprived mice treated with SS31, learning impairment was prevented, brain mitochondrial ATP levels and synaptic plasticity regulatory proteins were restored, and reactive oxygen species (ROS) and inflammatory cytokines levels were decreased in the hippocampus.
    2 context sentences
    Summary · context
    C57BL/6 female mice, 20 months old, were subcutaneously injected with SS31 (3 mg/kg) or saline daily for four days.
    Summary · context
    Sleep deprivation was 4 h daily for the last two days of SS31 treatment.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In sleep-deprived aging mice, SS31 decreased reactive oxygen species in the hippocampus.

    In plain wordsIn sleep-deprived older mice, SS31 lowered reactive oxygen species in a memory-related brain area.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseHippocampal reactive oxygen species
    Study

    mousen: not reported in source

    Short-term sleep deprivation in aging C57BL/6 mice (20 months)
    Comparator
    Saline
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    3 mg/kg · subcutaneous · daily for four daysAs given in this study (mouse).

    Source sentences

    Summary
    In sleep deprived mice treated with SS31, learning impairment was prevented, brain mitochondrial ATP levels and synaptic plasticity regulatory proteins were restored, and reactive oxygen species (ROS) and inflammatory cytokines levels were decreased in the hippocampus.
    2 context sentences
    Summary · context
    C57BL/6 female mice, 20 months old, were subcutaneously injected with SS31 (3 mg/kg) or saline daily for four days.
    Summary · context
    Sleep deprivation was 4 h daily for the last two days of SS31 treatment.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In sleep-deprived aging mice, SS31 decreased inflammatory cytokine levels in the hippocampus.

    In plain wordsIn sleep-deprived older mice, SS31 lowered inflammation markers in a memory-related brain area.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseHippocampal inflammatory cytokines
    Study

    mousen: not reported in source

    Short-term sleep deprivation in aging C57BL/6 mice (20 months)
    Comparator
    Saline
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    3 mg/kg · subcutaneous · daily for four daysAs given in this study (mouse).

    Source sentences

    Summary
    In sleep deprived mice treated with SS31, learning impairment was prevented, brain mitochondrial ATP levels and synaptic plasticity regulatory proteins were restored, and reactive oxygen species (ROS) and inflammatory cytokines levels were decreased in the hippocampus.
    2 context sentences
    Summary · context
    C57BL/6 female mice, 20 months old, were subcutaneously injected with SS31 (3 mg/kg) or saline daily for four days.
    Summary · context
    Sleep deprivation was 4 h daily for the last two days of SS31 treatment.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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