Novel humanin analogs confer neuroprotection and myoprotection to neuronal and myoblast cell cultures exposed to ischemia-like and doxorubicin-induced cell death insults.

Gilon C, Gitlin-Domagalska A, Lahiani A, Yehoshua-Alshanski S, Shumacher-Klinger A, Gilon D, Taha M, Sekler I, Hoffman A, Lazarovici P

Peptides1 Sept 2020Cited by 9 (Europe PMC)

PMID32889021DOI10.1016/j.peptides.2020.170399PepBankPUB-32889021

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

01LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
In neuronal cell cultures exposed to oxygen-glucose deprivation and reoxygenation, the humanin analogues HUJInin and c(D-Ser14-HN) conferred significant dose-dependent neuroprotection.
In plain wordsIn lab-grown nerve cells, two humanin analogues protected against damage from oxygen and glucose loss, with stronger effects at higher doses.
Peptide
Humanin
Outcome
DecreaseNeuronal cell death after OGD/R
Study
n: not reported in source
PC12 and SH-SY5Y neuronal cells after oxygen-glucose deprivation and reoxygenation
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
HUJInin and c(D-Ser14-HN) conferred significant dose-dependent neuroprotection
1 context sentence(design, population, supporting numbers)
Summary · context
The cellular models employed were oxygen-glucose-deprivation (OGD) followed by reoxygenation (R)-induced neurotoxicity in PC12 and SH-SY5Y neuronal cell cultures and Dox-induced cardiotoxicity in H9c2 and C2C12 myoblast cell cultures, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3288902101
02LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In neuronal cell cultures, the neuroprotection by HUJInin and c(D-Ser14-HN) was related to attenuation of OGD insult-induced Erk 1/2 phosphorylation.
In plain wordsIn lab-grown nerve cells, two humanin analogues lowered a stress-linked Erk 1/2 signal raised by oxygen and glucose loss.
Peptide
Humanin
Outcome
DecreaseErk 1/2 phosphorylation after OGD
Study
n: not reported in source
PC12 and SH-SY5Y neuronal cells after oxygen-glucose deprivation and reoxygenation
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
It was found that HUJInin and c(D-Ser14-HN) conferred significant dose-dependent neuroprotection, a phenomenon related to attenuation of OGD insult-induced Erk 1/2 phosphorylation, stimulation of AKT phosphorylation and improvement of mitochondrial functions.
1 context sentence(design, population, supporting numbers)
Summary · context
The cellular models employed were oxygen-glucose-deprivation (OGD) followed by reoxygenation (R)-induced neurotoxicity in PC12 and SH-SY5Y neuronal cell cultures and Dox-induced cardiotoxicity in H9c2 and C2C12 myoblast cell cultures, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3288902102
03LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In neuronal cell cultures, the neuroprotection by HUJInin and c(D-Ser14-HN) was related to stimulation of AKT phosphorylation.
In plain wordsIn lab-grown nerve cells, two humanin analogues increased AKT phosphorylation, a cell survival signal.
Peptide
Humanin
Outcome
IncreaseAKT phosphorylation
Study
n: not reported in source
PC12 and SH-SY5Y neuronal cells after oxygen-glucose deprivation and reoxygenation
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
It was found that HUJInin and c(D-Ser14-HN) conferred significant dose-dependent neuroprotection, a phenomenon related to attenuation of OGD insult-induced Erk 1/2 phosphorylation, stimulation of AKT phosphorylation and improvement of mitochondrial functions.
1 context sentence(design, population, supporting numbers)
Summary · context
The cellular models employed were oxygen-glucose-deprivation (OGD) followed by reoxygenation (R)-induced neurotoxicity in PC12 and SH-SY5Y neuronal cell cultures and Dox-induced cardiotoxicity in H9c2 and C2C12 myoblast cell cultures, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3288902103
04LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In neuronal cell cultures, the neuroprotection by HUJInin and c(D-Ser14-HN) was related to improvement of mitochondrial functions.
In plain wordsIn lab-grown nerve cells, two humanin analogues improved mitochondrial function.
Peptide
Humanin
Outcome
IncreaseMitochondrial function
Study
n: not reported in source
PC12 and SH-SY5Y neuronal cells after oxygen-glucose deprivation and reoxygenation
Outcome group
Mitochondrial function · Nervous system and cognition
Source sentences
Summary
It was found that HUJInin and c(D-Ser14-HN) conferred significant dose-dependent neuroprotection, a phenomenon related to attenuation of OGD insult-induced Erk 1/2 phosphorylation, stimulation of AKT phosphorylation and improvement of mitochondrial functions.
1 context sentence(design, population, supporting numbers)
Summary · context
The cellular models employed were oxygen-glucose-deprivation (OGD) followed by reoxygenation (R)-induced neurotoxicity in PC12 and SH-SY5Y neuronal cell cultures and Dox-induced cardiotoxicity in H9c2 and C2C12 myoblast cell cultures, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3288902104
05LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
In myoblast cell cultures, the analogues HUJInin and c(D-Ser14-HN) conferred a myoprotective effect against doxorubicin-induced apo-necrotic cell death.
In plain wordsIn lab-grown muscle cells, two humanin analogues protected against cell death caused by doxorubicin.
Peptide
Humanin
Outcome
DecreaseDoxorubicin-induced apo-necrotic cell death
Study
n: not reported in source
H9c2 and C2C12 myoblast cells exposed to doxorubicin
Outcome group
Heart function · Cardiovascular
Source sentences
Summary
These peptides also conferred myoprotective effect towards Dox-induced apo-necrotic cell death insults.
1 context sentence(design, population, supporting numbers)
Summary · context
The cellular models employed were oxygen-glucose-deprivation (OGD) followed by reoxygenation (R)-induced neurotoxicity in PC12 and SH-SY5Y neuronal cell cultures and Dox-induced cardiotoxicity in H9c2 and C2C12 myoblast cell cultures, respectively.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3288902105

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

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