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Study · In vitro (cells) · 2020

3 results

Targeting Membrane HDM-2 by PNC-27 Induces Necrosis in Leukemia Cells But Not in Normal Hematopoietic Cells.

Thadi A, Lewis L, Goldstein E, Aggarwal A, Khalili M, Steele L, Polyak B, Seydafkan S, Bluth MH, Ward KA, Styler M, Campbell PM, Pincus MR, Bowne WB

Anticancer research1 Sept 2020Cited by 1 (Europe PMC)

PMID32878773 (opens in a new tab)DOI10.21873/anticanres.14488 (opens in a new tab)PepBankPUB-32878773
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In leukemia cell lines, PNC‑27 bound to membrane HDM-2.

    In plain wordsIn cells, PNC‑27 bound to membrane HDM-2 in leukemia cells.

    Peptide
    PNC-27
    Outcome
    Not applicableMembrane HDM-2 binding
    Study

    humann: not reported in source

    U937, OCI-AML3 and HL60 leukemia cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated · 4 hAs given in this study (human).

    Source sentences

    Summary
    PNC-27 can bind to membrane HDM-2 to induce cell necrosis and LDH release within 4 h.
    2 context sentences
    Summary · context
    Herein, we investigated HDM-2 membrane expression and the effect of PNC-27 treatment on human non-stem cell acute myelogenous leukemia cell lines: U937, acute monocytic leukemia; OCI-AML3, acute myelomonocytic leukemia and HL60, acute promyelocytic leukemia.
    Summary · context
    Cell viability was assessed using MTT assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers annexin V and caspase-3.
  2. 02LabIn vitro (cells)Efficacy outcome

    In leukemia cell lines, PNC‑27 induced necrosis within 4 h.

    In plain wordsIn cells, PNC‑27 caused leukemia cell necrosis within 4 h.

    Peptide
    PNC-27
    Outcome
    IncreaseCell necrosis
    Study

    humann: not reported in source

    U937, OCI-AML3 and HL60 leukemia cells
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated · 4 hAs given in this study (human).

    Source sentences

    Summary
    PNC-27 can bind to membrane HDM-2 to induce cell necrosis and LDH release within 4 h.
    2 context sentences
    Summary · context
    Herein, we investigated HDM-2 membrane expression and the effect of PNC-27 treatment on human non-stem cell acute myelogenous leukemia cell lines: U937, acute monocytic leukemia; OCI-AML3, acute myelomonocytic leukemia and HL60, acute promyelocytic leukemia.
    Summary · context
    Cell viability was assessed using MTT assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers annexin V and caspase-3.
  3. 03LabIn vitro (cells)Mechanism

    In leukemia cell lines, PNC‑27 induced LDH release within 4 h.

    In plain wordsIn cells, PNC‑27 caused LDH release within 4 h.

    Peptide
    PNC-27
    Outcome
    IncreaseLDH release
    Study

    humann: not reported in source

    U937, OCI-AML3 and HL60 leukemia cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated · 4 hAs given in this study (human).

    Source sentences

    Summary
    PNC-27 can bind to membrane HDM-2 to induce cell necrosis and LDH release within 4 h.
    2 context sentences
    Summary · context
    Herein, we investigated HDM-2 membrane expression and the effect of PNC-27 treatment on human non-stem cell acute myelogenous leukemia cell lines: U937, acute monocytic leukemia; OCI-AML3, acute myelomonocytic leukemia and HL60, acute promyelocytic leukemia.
    Summary · context
    Cell viability was assessed using MTT assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers annexin V and caspase-3.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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