Comparison of different protein precipitation and solid-phase extraction protocols for the study of the catabolism of peptide drugs by LC-HRMS.

Esposito S, Vanni D, Menta S, Orsatti L, Monteagudo E

Journal of peptide science : an official publication of the European Peptide Society6 Jul 2020Cited by 7 (Europe PMC)

PMID32633064DOI10.1002/psc.3272PepBankPUB-32633064

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Liraglutide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Liraglutide.

01LabNot a test of Liraglutide aloneIn vitro (cell-free)Other
In spiked human plasma, protein precipitation with three volumes of ACN or EtOH gave the highest overall recoveries, above 50% for the four parent peptides, liraglutide among them, and all their catabolites.
In plain wordsIn lab tests with spiked plasma, protein precipitation with ACN or EtOH recovered over 50% of four test peptides, liraglutide included, and their breakdown products.
Peptide
Liraglutide
Outcome
Not applicableRecovery with protein precipitation
Study
n: not reported in source
Human plasma spiked with trypsin and chymotrypsin digests of model peptides
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Recovery
> 50%
PP with three volumes of ACN or EtOH
Source sentences
Summary
PP with three volumes of ACN or EtOH yielded the highest overall recoveries (more than 50% for the four parent peptides and all their catabolites) among all the tested PP and SPE protocols.
2 context sentences(design, population, supporting numbers)
Summary · context
To this purpose, four model peptides representative of different classes (somatostatin, GLP-2, human insulin and liraglutide) were digested with trypsin and chymotrypsin to simulate proteolytic catabolism.
Summary · context
The resulting mixtures of the parent peptides and their proteolytic products covering a wide range of relative hydrophobicity (H_R ) and isoelectric points (pI) were spiked in human plasma and underwent different PP and SPE protocols.
In this study, Liraglutide was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Liraglutide.
Report a problemFND-3263306401
02LabNot a test of Liraglutide aloneIn vitro (cell-free)Other
In spiked human plasma, mixed-mode anion exchange was the only one of five tested SPE sorbents that extracted all the peptides, liraglutide among them, with recoveries above 20%.
In plain wordsIn lab tests with spiked plasma, only one of five extraction sorbents recovered all test peptides, liraglutide included, at more than 20%.
Peptide
Liraglutide
Outcome
Not applicableRecovery with solid-phase extraction sorbents
Study
n: not reported in source
Human plasma spiked with trypsin and chymotrypsin digests of model peptides
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Recovery
> 20%
mixed-mode anion exchange SPE
Source sentences
Summary
Mixed-mode anion exchange (MAX) was the only SPE sorbent among the five tested that allowed to extract all the peptides with recoveries more than 20%.
2 context sentences(design, population, supporting numbers)
Summary · context
To this purpose, four model peptides representative of different classes (somatostatin, GLP-2, human insulin and liraglutide) were digested with trypsin and chymotrypsin to simulate proteolytic catabolism.
Summary · context
The resulting mixtures of the parent peptides and their proteolytic products covering a wide range of relative hydrophobicity (H_R ) and isoelectric points (pI) were spiked in human plasma and underwent different PP and SPE protocols.
In this study, Liraglutide was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Liraglutide.
Report a problemFND-3263306402
03LabNot a test of Liraglutide aloneIn vitro (cell-free)Other
In spiked human plasma, the matrix effect was generally lower with SPE.
In plain wordsIn lab tests with spiked plasma, extraction on a sorbent generally disturbed the measurement less than protein precipitation.
Peptide
Liraglutide
Outcome
DecreaseMatrix effect with SPE versus protein precipitation
Study
n: not reported in source
Human plasma spiked with trypsin and chymotrypsin digests of model peptides
Comparator
Protein precipitation
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Matrix effect was generally lower with SPE.
2 context sentences(design, population, supporting numbers)
Summary · context
To this purpose, four model peptides representative of different classes (somatostatin, GLP-2, human insulin and liraglutide) were digested with trypsin and chymotrypsin to simulate proteolytic catabolism.
Summary · context
The resulting mixtures of the parent peptides and their proteolytic products covering a wide range of relative hydrophobicity (H_R ) and isoelectric points (pI) were spiked in human plasma and underwent different PP and SPE protocols.
In this study, Liraglutide was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Liraglutide.
Report a problemFND-3263306403
04LabNot a test of Liraglutide aloneIn vitro (cell-free)Other
In spiked human plasma, lipidated liraglutide catabolites, being hydrophobic, had a much narrower choice of protein precipitation solvent or SPE protocol.
In plain wordsIn lab tests with spiked plasma, breakdown products of liraglutide could be extracted with fewer methods than other peptides.
Peptide
Liraglutide
Outcome
Not applicableExtraction options for hydrophobic liraglutide catabolites
Study
n: not reported in source
Human plasma spiked with trypsin and chymotrypsin digests of model peptides
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
peptides with either high hydrophilicity (e.g., somatostatin catabolites) or hydrophobicity (GLP-2 and lipidated liraglutide catabolites) had a much narrower choice of PP solvent or SPE protocol.
2 context sentences(design, population, supporting numbers)
Summary · context
To this purpose, four model peptides representative of different classes (somatostatin, GLP-2, human insulin and liraglutide) were digested with trypsin and chymotrypsin to simulate proteolytic catabolism.
Summary · context
The resulting mixtures of the parent peptides and their proteolytic products covering a wide range of relative hydrophobicity (H_R ) and isoelectric points (pI) were spiked in human plasma and underwent different PP and SPE protocols.
In this study, Liraglutide was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Liraglutide.
Report a problemFND-3263306404

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 11:11 UTC
Study results updated
Version 1
9 Oct 2026, 03:05 UTC
First added · source update from Europe PMC

Subjects

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