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Study · Animal study (in vivo) · mouse · 2020

10 results

Humanin Promotes Tumor Progression in Experimental Triple Negative Breast Cancer.

Moreno Ayala MA, Gottardo MF, Zuccato CF, Pidre ML, Nicola Candia AJ, Asad AS, Imsen M, Romanowski V, Creton A, Isla Larrain M, Seilicovich A, Candolfi M

Scientific reports22 May 2020Cited by 31 (Europe PMC)

PMID32444831 (opens in a new tab)PMCIDPMC7244539 (opens in a new tab)DOI10.1038/s41598-020-65381-7 (opens in a new tab)PepBankPUB-32444831
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Summary

Humanin (HN) is a mitochondrial-derived peptide with cytoprotective effect in many tissues. Administration of HN analogs has been proposed as therapeutic approach for degenerative diseases. Although HN has been shown to protect normal tissues from chemotherapy, its role in tumor pathogenesis is poorly understood. Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC). The meta-analysis of transcriptomic data from The Cancer Genome Atlas indicated that HN and its receptors are expressed in breast cancer specimens. By immunohistochemistry we observed up-regulation of HN in TNBC biopsies when compared to mammary gland sections from healthy donors. Addition of exogenous HN protected TNBC cells from apoptotic stimuli whereas shRNA-mediated HN silencing reduced their viability and enhanced their chemo-sensitivity. Systemic administration of HN in TNBC-bearing mice reduced tumor apoptotic rate, impaired the antitumor and anti-metastatic effect of chemotherapy and stimulated tumor progression, accelerating tumor growth and development of spontaneous lung metastases. These findings suggest that HN may exert pro-tumoral effects and thus, caution should be taken when using exogenous HN to treat degenerative diseases. In addition, our study suggests that HN blockade could constitute a therapeutic strategy to improve the efficacy of chemotherapy in breast cancer.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    Systemic humanin in TNBC-bearing mice reduced the tumor apoptotic rate.

    In plain wordsIn mice with breast cancer, humanin lowered the rate of cell death in tumors.

    Peptide
    Humanin
    Outcome
    DecreaseTumor apoptotic rate
    Study

    mousen: not reported in source

    TNBC-bearing mice
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Systemic administration of HN in TNBC-bearing mice reduced tumor apoptotic rate, impaired the antitumor and anti-metastatic effect of chemotherapy and stimulated tumor progression, accelerating tumor growth and development of spontaneous lung metastases.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    Systemic humanin in TNBC-bearing mice impaired the antitumor effect of chemotherapy.

    In plain wordsIn mice with breast cancer, humanin weakened the tumor-shrinking effect of chemotherapy.

    Peptide
    Humanin
    Outcome
    DecreaseAntitumor effect of chemotherapy
    Study

    mousen: not reported in source

    TNBC-bearing mice
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Systemic administration of HN in TNBC-bearing mice reduced tumor apoptotic rate, impaired the antitumor and anti-metastatic effect of chemotherapy and stimulated tumor progression, accelerating tumor growth and development of spontaneous lung metastases.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    Systemic humanin in TNBC-bearing mice impaired the anti-metastatic effect of chemotherapy.

    In plain wordsIn mice with breast cancer, humanin weakened the effect of chemotherapy against spread of cancer.

    Peptide
    Humanin
    Outcome
    DecreaseAnti-metastatic effect of chemotherapy
    Study

    mousen: not reported in source

    TNBC-bearing mice
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Systemic administration of HN in TNBC-bearing mice reduced tumor apoptotic rate, impaired the antitumor and anti-metastatic effect of chemotherapy and stimulated tumor progression, accelerating tumor growth and development of spontaneous lung metastases.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    Systemic humanin in TNBC-bearing mice accelerated tumor growth.

    In plain wordsIn mice with breast cancer, humanin made tumors grow faster.

    Peptide
    Humanin
    Outcome
    IncreaseTumor growth
    Study

    mousen: not reported in source

    TNBC-bearing mice
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Systemic administration of HN in TNBC-bearing mice reduced tumor apoptotic rate, impaired the antitumor and anti-metastatic effect of chemotherapy and stimulated tumor progression, accelerating tumor growth and development of spontaneous lung metastases.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    Systemic humanin in TNBC-bearing mice accelerated the development of spontaneous lung metastases.

    In plain wordsIn mice with breast cancer, humanin sped up the spread of cancer to the lungs.

    Peptide
    Humanin
    Outcome
    IncreaseSpontaneous lung metastases
    Study

    mousen: not reported in source

    TNBC-bearing mice
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Systemic administration of HN in TNBC-bearing mice reduced tumor apoptotic rate, impaired the antitumor and anti-metastatic effect of chemotherapy and stimulated tumor progression, accelerating tumor growth and development of spontaneous lung metastases.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).
  6. 06LabIn vitro (cells)Efficacy outcome

    Exogenous humanin protected triple negative breast cancer cells from apoptotic stimuli.

    In plain wordsIn breast cancer cells, added humanin protected the cells from signals that cause cell death.

    Peptide
    Humanin
    Outcome
    DecreaseApoptosis from apoptotic stimuli
    Study

    n: not reported in source

    TNBC cells
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Addition of exogenous HN protected TNBC cells from apoptotic stimuli whereas shRNA-mediated HN silencing reduced their viability and enhanced their chemo-sensitivity.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 07HumanEndogenous levelObservational studyOther

    Meta-analysis of transcriptomic data from The Cancer Genome Atlas indicated that humanin and its receptors are expressed in breast cancer specimens.

    In plain wordsIn breast cancer samples, the body's own humanin and its receptors were present.

    Peptide
    Humanin
    Outcome
    Not applicableHumanin and receptor expression in breast cancer specimens
    Study

    n: not reported in source

    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    The meta-analysis of transcriptomic data from The Cancer Genome Atlas indicated that HN and its receptors are expressed in breast cancer specimens.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  2. 08HumanEndogenous levelObservational studyOther

    Immunohistochemistry showed up-regulation of humanin in triple negative breast cancer biopsies compared with mammary gland sections from healthy donors.

    In plain wordsIn triple negative breast cancer samples, the body's own humanin was higher than in healthy breast tissue.

    Peptide
    Humanin
    Outcome
    IncreaseHumanin expression in TNBC biopsies
    Study

    n: not reported in source

    Comparator
    Mammary gland sections from healthy donors
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    By immunohistochemistry we observed up-regulation of HN in TNBC biopsies when compared to mammary gland sections from healthy donors.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

Studies that did not test the peptide alone2

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 09LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome

    shRNA-mediated humanin silencing reduced the viability of triple negative breast cancer cells.

    In plain wordsIn breast cancer cells, switching off humanin with shRNA lowered cell survival.

    Peptide
    Humanin
    Outcome
    DecreaseCell viability after humanin silencing
    Study

    n: not reported in source

    TNBC cells
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    Addition of exogenous HN protected TNBC cells from apoptotic stimuli whereas shRNA-mediated HN silencing reduced their viability and enhanced their chemo-sensitivity.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 10LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome

    shRNA-mediated humanin silencing enhanced the chemo-sensitivity of triple negative breast cancer cells.

    In plain wordsIn breast cancer cells, switching off humanin with shRNA made cells more sensitive to chemotherapy.

    Peptide
    Humanin
    Outcome
    IncreaseChemo-sensitivity after humanin silencing
    Study

    n: not reported in source

    TNBC cells
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    Addition of exogenous HN protected TNBC cells from apoptotic stimuli whereas shRNA-mediated HN silencing reduced their viability and enhanced their chemo-sensitivity.
    1 context sentence
    Summary · context
    Here, we evaluated the effect of HN on the progression of experimental triple negative breast cancer (TNBC).

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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