Humanin Blocks the Aggregation of Amyloid-β Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3.

Price D, Dorandish S, Williams A, Iwaniec B, Stephens A, Marshall K, Guthrie J, Heyl D, Evans HG

Biochemistry20 May 2020Cited by 24 (Europe PMC)

PMID32383868PMCIDPMC8193794DOI10.1021/acs.biochem.0c00274PepBankPUB-32383868

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cell-free)Mechanism
In vitro, humanin and AChE bound amyloid-beta simultaneously, but not in the presence of IGFBP-3.
In plain wordsIn lab tests, humanin and AChE could bind amyloid-beta together, unless IGFBP-3 was present.
Peptide
Humanin
Outcome
Not applicableSimultaneous binding of humanin and AChE to amyloid-beta
Study
n: not reported in source
in vitro binding assays
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
HN and AChE can simultaneously bind Aβ but not when in the presence of IGFBP-3
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
Report a problemFND-3238386802
02LabIn vitro (cell-free)Efficacy outcome
In vitro, humanin was unable to reduce amyloid-beta aggregation in the presence of IGFBP-3.
In plain wordsIn lab tests, humanin could not reduce amyloid-beta clumping when IGFBP-3 was present.
Peptide
Humanin
Outcome
No significant changeAmyloid-beta aggregation with IGFBP-3 present
Study
n: not reported in source
in vitro aggregation assays
Comparator
Humanin without IGFBP-3
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
HN is unable to reduce the aggregation of Aβ in the presence of IGFBP-3
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
Report a problemFND-3238386803
03LabIn vitro (cell-free)Efficacy outcome
In vitro, humanin abolished the amyloid-beta aggregation induced by AChE in the absence of IGFBP-3.
In plain wordsIn lab tests, humanin stopped AChE-driven amyloid-beta clumping when IGFBP-3 was absent.
Peptide
Humanin
Outcome
DecreaseAChE-induced amyloid-beta aggregation
Study
n: not reported in source
in vitro aggregation assays
Comparator
AChE alone
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
HN abolishes the aggregation of Aβ induced by the addition of AChE in the absence of IGFBP-3.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
Report a problemFND-3238386804

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

04LabEndogenous levelIn vitro (cells)Mechanism
In conditioned media from A549 and H1299 lung cancer cells, AChE and the cells' own humanin bound amyloid-beta simultaneously.
In plain wordsIn lung cancer cell media, AChE and the cells' own humanin could bind amyloid-beta at the same time.
Peptide
Humanin
Outcome
Not applicableSimultaneous binding of AChE and humanin to amyloid-beta in media
Study
humann: not reported in source
conditioned media from A549 and H1299 lung cancer cells
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Source sentences
Summary
In the media, AChE and HN can simultaneously bind Aβ.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3238386805

Studies that did not test the peptide alone6

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

05LabNot a test of Humanin aloneIn vitro (cells)Mechanism
Exogenously added IGFBP-3 reduced the amount of humanin found in a complex in the media.
In plain wordsIn lung cancer cell media, added IGFBP-3 reduced the amount of humanin found in a complex with amyloid-beta.
Peptide
Humanin
Outcome
DecreaseHumanin found in complex after exogenous IGFBP-3
Study
humann: not reported in source
conditioned media from A549 and H1299 lung cancer cells
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Source sentences
Summary
Exogenously added IGFBP-3 reduced the amount of HN found in a complex when using 6E10 antibodies and correlated with a concomitant increase in the amyloid oligomers.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3238386806
06LabNot a test of Humanin aloneIn vitro (cells)Mechanism
Exogenous IGFBP-3 correlated with a concomitant increase in amyloid oligomers in the media.
In plain wordsIn lung cancer cell media, added IGFBP-3 went with more amyloid oligomers.
Peptide
Humanin
Outcome
IncreaseAmyloid oligomers after exogenous IGFBP-3
Study
humann: not reported in source
conditioned media from A549 and H1299 lung cancer cells
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Exogenously added IGFBP-3 reduced the amount of HN found in a complex when using 6E10 antibodies and correlated with a concomitant increase in the amyloid oligomers.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3238386807
07LabNot a test of Humanin aloneIn vitro (cells)Mechanism
Immunodepletion of humanin from the media increased the relative abundance of amyloid-beta oligomers, including A11-positive prefibrillar oligomers.
In plain wordsIn lung cancer cell media, removing humanin raised the share of amyloid-beta oligomers.
Peptide
Humanin
Outcome
IncreaseOligomer abundance after humanin immunodepletion
Study
humann: not reported in source
A549 and H1299 lung cancer cell media
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Immunodepletion of HN from the media of the A549 and H1299 cells increased the relative abundance of the oligomer vs the total amount of Aβ, the A11-positive prefibrillar oligomers, and to a lesser extent the LOC-positive fibrillar oligomers, and was also correlated with diminished cell viability an…
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3238386808
08LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
Immunodepletion of humanin from the media correlated with diminished cell viability.
In plain wordsIn lung cancer cells, removing humanin from the media went with lower cell viability.
Peptide
Humanin
Outcome
DecreaseCell viability after humanin immunodepletion
Study
humann: not reported in source
A549 and H1299 lung cancer cell media
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Immunodepletion of HN from the media of the A549 and H1299 cells increased the relative abundance of the oligomer vs the total amount of Aβ, the A11-positive prefibrillar oligomers, and to a lesser extent the LOC-positive fibrillar oligomers, and was also correlated with diminished cell viability an…
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3238386809
09LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
Immunodepletion of humanin from the media correlated with increased apoptosis.
In plain wordsIn lung cancer cells, removing humanin from the media went with more cell death.
Peptide
Humanin
Outcome
IncreaseApoptosis after humanin immunodepletion
Study
humann: not reported in source
A549 and H1299 lung cancer cell media
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Immunodepletion of HN from the media of the A549 and H1299 cells increased the relative abundance of the oligomer vs the total amount of Aβ, the A11-positive prefibrillar oligomers, and to a lesser extent the LOC-positive fibrillar oligomers, and was also correlated with diminished cell viability an…
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3238386810
10LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism
In vitro, IGFBP-3 bound humanin and blocked it from binding amyloid-beta, with or without AChE.
In plain wordsIn lab tests, IGFBP-3 bound humanin and stopped it from binding amyloid-beta.
Peptide
Humanin
Outcome
Not applicableIGFBP-3 binding to humanin and humanin-Abeta binding
Study
n: not reported in source
in vitro binding assays
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Source sentences
Summary
IGFBP-3 binds HN and blocks it from binding Aβ in the absence or presence of AChE
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we examined the regulation of Aβ conformations by HN, AChE, and IGFBP-3 both in vitro and in the conditioned media from A549 and H1299 lung cancer cells.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3238386801

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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