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Study · Animal study (in vivo) · rat · 2020

6 results

Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4.

Poh KK, Lee PSS, Djohan AH, Galupo MJ, Songco GG, Yeo TC, Tan HC, Richards AM, Ye L

Cells12 Apr 2020Cited by 19 (Europe PMC)

PMID32290541 (opens in a new tab)PMCIDPMC7226991 (opens in a new tab)DOI10.3390/cells9040949 (opens in a new tab)PepBankPUB-32290541
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Summary

Endothelial progenitor cells (EPCs) are bone-marrow derived cells that are critical in the maintenance of endothelial wall integrity and protection of ischemic myocardium through the formation of new blood vessels (vasculogenesis) or proliferation of pre-existing vasculature (angiogenesis). Diabetes mellitus (DM) and the metabolic syndrome are commonly associated with ischemic heart disease through its pathological effects on the endothelium and consequent endothelial dysfunction. Thymosin-β4 (Tβ4) which expressed in the embryonic heart is critical in epicardial and coronary artery formation. In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo. It was found that 10 ng/mL Tβ4 increased migration, tubule formation, and angiogenic factor secretion of diabetic EPCs in vitro. In vivo, although implantation of Tβ4 treated diabetic EPCs significantly increased capillary density and attracted more c-Kit positive progenitor cells into the infarcted hearts as compared with implantation of non-Tβ4 treated diabetic EPCs, the significantly improved left ventricular ejection fraction was only found in the rats which received non-Tβ4 treated EPCs. The data suggests that a low dose Tβ4 increases diabetic EPC migration, tubule formation, and angiogenic factor secretion. However, it did not improve the effects of EPCs on left ventricular pump function in diabetic rats with MI.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In diabetic rats after myocardial infarction, implanting Tβ4-treated EPCs significantly increased capillary density compared with non-Tβ4 treated EPCs.

    In plain wordsIn diabetic rats after a heart attack, EPCs treated with Tβ4 gave more capillaries than EPCs without Tβ4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseCapillary density in infarcted hearts
    Study

    ratn: not reported in source

    diabetic rats with myocardial infarction
    Comparator
    EPCs without Tβ4 treatment
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vivo, although implantation of Tβ4 treated diabetic EPCs significantly increased capillary density and attracted more c-Kit positive progenitor cells into the infarcted hearts as compared with implantation of non-Tβ4 treated diabetic EPCs
    1 context sentence
    Summary · context
    In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In diabetic rats after myocardial infarction, implanting Tβ4-treated EPCs attracted more c-Kit positive progenitor cells into infarcted hearts than non-Tβ4 treated EPCs.

    In plain wordsIn diabetic rats after a heart attack, EPCs treated with Tβ4 drew more progenitor cells to the heart than EPCs without Tβ4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Increasec-Kit positive progenitor cells in infarcted hearts
    Study

    ratn: not reported in source

    diabetic rats with myocardial infarction
    Comparator
    EPCs without Tβ4 treatment
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vivo, although implantation of Tβ4 treated diabetic EPCs significantly increased capillary density and attracted more c-Kit positive progenitor cells into the infarcted hearts as compared with implantation of non-Tβ4 treated diabetic EPCs
    1 context sentence
    Summary · context
    In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In diabetic rats after myocardial infarction, Tβ4-treated EPCs did not improve left ventricular ejection fraction, which improved only with non-Tβ4 treated EPCs.

    In plain wordsIn diabetic rats after a heart attack, EPCs treated with Tβ4 did not improve heart pumping, but EPCs without Tβ4 did.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeLeft ventricular ejection fraction
    Study

    ratn: not reported in source

    diabetic rats with myocardial infarction
    Comparator
    EPCs without Tβ4 treatment
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the significantly improved left ventricular ejection fraction was only found in the rats which received non-Tβ4 treated EPCs.
    2 context sentences
    Summary · context
    However, it did not improve the effects of EPCs on left ventricular pump function in diabetic rats with MI.
    Summary · context
    In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo.
  4. 04LabIn vitro (cells)Efficacy outcome

    In diabetic endothelial progenitor cells, 10 ng/mL Tβ4 increased migration.

    In plain wordsIn cells from diabetic sources, Tβ4 increased cell movement.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseDiabetic EPC migration
    Study

    n: not reported in source

    diabetic endothelial progenitor cells (EPCs)
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    10 ng/mLAs given in this study.

    Source sentences

    Summary
    It was found that 10 ng/mL Tβ4 increased migration, tubule formation, and angiogenic factor secretion of diabetic EPCs in vitro.
    1 context sentence
    Summary · context
    In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo.
  5. 05LabIn vitro (cells)Efficacy outcome

    In diabetic endothelial progenitor cells, 10 ng/mL Tβ4 increased tubule formation.

    In plain wordsIn cells from diabetic sources, Tβ4 increased the formation of vessel-like tubes.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseDiabetic EPC tubule formation
    Study

    n: not reported in source

    diabetic endothelial progenitor cells (EPCs)
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    10 ng/mLAs given in this study.

    Source sentences

    Summary
    It was found that 10 ng/mL Tβ4 increased migration, tubule formation, and angiogenic factor secretion of diabetic EPCs in vitro.
    1 context sentence
    Summary · context
    In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo.
  6. 06LabIn vitro (cells)Efficacy outcome

    In diabetic endothelial progenitor cells, 10 ng/mL Tβ4 increased angiogenic factor secretion.

    In plain wordsIn cells from diabetic sources, Tβ4 increased the release of blood vessel growth factors.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseDiabetic EPC angiogenic factor secretion
    Study

    n: not reported in source

    diabetic endothelial progenitor cells (EPCs)
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    10 ng/mLAs given in this study.

    Source sentences

    Summary
    It was found that 10 ng/mL Tβ4 increased migration, tubule formation, and angiogenic factor secretion of diabetic EPCs in vitro.
    1 context sentence
    Summary · context
    In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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