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Study · Measured the body's own peptide · 2020

8 results

High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men.

Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL

Journal of applied physiology (Bethesda, Md. : 1985)9 Apr 2020Cited by 44 (Europe PMC)

PMID32271093 (opens in a new tab)PMCIDPMC7717117 (opens in a new tab)DOI10.1152/japplphysiol.00032.2020 (opens in a new tab)PepBankPUB-32271093
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

No result from this study comes from a test of the peptide.

Natural levels measured8

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01HumanEndogenous levelNon-randomized trialOther

    In young healthy untrained men, acute high-intensity interval exercise increased humanin protein abundance in muscle.

    In plain wordsIn young healthy men, one hard exercise session raised the body's own humanin protein in muscle.

    Peptide
    Humanin
    Outcome
    IncreaseMuscle humanin protein after acute high-intensity exercise
    Study

    n = 10

    Comparator
    Before exercise
    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    Humanin protein abundance in muscle and plasma were increased following an acute high-intensity exercise bout.
    1 context sentence
    Summary · context
    Vastus lateralis muscle biopsies and plasma samples were collected from young healthy untrained men (n = 10, 24.5 ± 3.7 yr) before, immediately following, and 4 h following the completion of 10 × 60 s cycle ergometer bouts at V̇o2peak power output (untrained).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  2. 02HumanEndogenous levelNon-randomized trialOther

    In young healthy untrained men, acute high-intensity interval exercise increased plasma humanin.

    In plain wordsIn young healthy men, one hard exercise session raised the body's own humanin in blood.

    Peptide
    Humanin
    Outcome
    IncreasePlasma humanin after acute high-intensity exercise
    Study

    n = 10

    Comparator
    Before exercise
    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    Humanin protein abundance in muscle and plasma were increased following an acute high-intensity exercise bout.
    1 context sentence
    Summary · context
    Vastus lateralis muscle biopsies and plasma samples were collected from young healthy untrained men (n = 10, 24.5 ± 3.7 yr) before, immediately following, and 4 h following the completion of 10 × 60 s cycle ergometer bouts at V̇o2peak power output (untrained).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  3. 03HumanEndogenous levelNon-randomized trialOther

    Six sessions of HIIT over 2 weeks showed only a trend (P = 0.063) towards lower absolute plasma humanin levels.

    In plain wordsIn healthy men, two weeks of interval training did not clearly change the body's own humanin in blood.

    Peptide
    Humanin
    Outcome
    No significant changeAbsolute plasma humanin after HIIT training
    Study

    n = 10

    Comparator
    Untrained state
    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    HIIT trended (P = 0.063) to lower absolute humanin plasma levels, without effecting the response in muscle or plasma to acute exercise.
    1 context sentence
    Summary · context
    Resting and postexercise sampling was also performed after six HIIT sessions (trained) completed over 2 wk.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  4. 04HumanEndogenous levelNon-randomized trialOther

    HIIT did not alter the muscle or plasma humanin response to acute exercise.

    In plain wordsIn healthy men, interval training did not change how the body's own humanin responded to a hard exercise session.

    Peptide
    Humanin
    Outcome
    No significant changeEffect of HIIT on humanin response to acute exercise
    Study

    n = 10

    Comparator
    Untrained state
    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    HIIT trended (P = 0.063) to lower absolute humanin plasma levels, without effecting the response in muscle or plasma to acute exercise.
    1 context sentence
    Summary · context
    Resting and postexercise sampling was also performed after six HIIT sessions (trained) completed over 2 wk.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  5. 05HumanEndogenous levelNon-randomized trialOther

    Muscle and plasma humanin levels showed a weak positive correlation.

    In plain wordsIn healthy men, the body's own humanin in muscle and in blood were weakly linked.

    Peptide
    Humanin
    Outcome
    Not applicableCorrelation between muscle and plasma humanin
    Study

    n = 10

    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    There was a weak positive correlation between muscle and plasma humanin levels
    1 context sentence
    Summary · context
    Vastus lateralis muscle biopsies and plasma samples were collected from young healthy untrained men (n = 10, 24.5 ± 3.7 yr) before, immediately following, and 4 h following the completion of 10 × 60 s cycle ergometer bouts at V̇o2peak power output (untrained).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  6. 06HumanEndogenous levelNon-randomized trialOther

    The increase in muscle humanin with acute exercise was not associated with MT-RNR2 or humanin mRNA levels.

    In plain wordsIn healthy men, the exercise-related rise in the body's own muscle humanin was not linked to changes in its mRNA.

    Peptide
    Humanin
    Outcome
    No significant changeAssociation of muscle humanin increase with mRNA levels
    Study

    n = 10

    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    The increase in muscle humanin levels with acute exercise was not associated with MT-RNR2 mRNA or humanin mRNA levels (which decreased following acute exercise).
    1 context sentence
    Summary · context
    Vastus lateralis muscle biopsies and plasma samples were collected from young healthy untrained men (n = 10, 24.5 ± 3.7 yr) before, immediately following, and 4 h following the completion of 10 × 60 s cycle ergometer bouts at V̇o2peak power output (untrained).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  7. 07HumanEndogenous levelNon-randomized trialOther

    MT-RNR2 mRNA and humanin mRNA levels in muscle decreased following acute exercise.

    In plain wordsIn healthy men, the body's own humanin mRNA levels in muscle fell after a hard exercise session.

    Peptide
    Humanin
    Outcome
    DecreaseMT-RNR2 and humanin mRNA after acute exercise
    Study

    n = 10

    Comparator
    Before exercise
    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    The increase in muscle humanin levels with acute exercise was not associated with MT-RNR2 mRNA or humanin mRNA levels (which decreased following acute exercise).
    1 context sentence
    Summary · context
    Vastus lateralis muscle biopsies and plasma samples were collected from young healthy untrained men (n = 10, 24.5 ± 3.7 yr) before, immediately following, and 4 h following the completion of 10 × 60 s cycle ergometer bouts at V̇o2peak power output (untrained).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  8. 08LabEndogenous levelEx vivo tissueOther

    Contraction of isolated mouse EDL muscle increased humanin levels about 4-fold.

    In plain wordsIn isolated mouse muscle, contraction raised the muscle's own humanin levels about 4-fold.

    Peptide
    Humanin
    Outcome
    IncreaseHumanin levels after muscle contraction
    Study

    mousen: not reported in source

    isolated mouse EDL muscle
    Outcome group
    Mitochondrial function · Whole body

    Source sentences

    Summary
    contraction of isolated mouse EDL muscle increased humanin levels ~4-fold.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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