Skip to content

Study · Animal study (in vivo) · mouse · 2020

9 results

Thymosin β4-Enhancing Therapeutic Efficacy of Human Adipose-Derived Stem Cells in Mouse Ischemic Hindlimb Model.

Kim JH, Lim IR, Park CY, Joo HJ, Noh JM, Choi SC, Hong SJ, Lim DS

International journal of molecular sciences21 Mar 2020Cited by 9 (Europe PMC)

PMID32245208 (opens in a new tab)PMCIDPMC7139370 (opens in a new tab)DOI10.3390/ijms21062166 (opens in a new tab)PepBankPUB-32245208
Download PDFPubMedAsk about this study
Jump to 9 results

Summary

Thymosin β4 (Tβ4) is a G-actin sequestering protein that contributes to diverse cellular activities, such as migration and angiogenesis. In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated. We observed that exogenous treatment with Tβ4 enhanced endogenous TMSB4X mRNA expression and promoted morphological changes (increased cell length) in hASCs. Interestingly, Tβ4 induced the active state of hASCs by up-regulating intracellular signaling pathways including the PI3K/AKT/mTOR and MAPK/ERK pathways. Treatment with Tβ4 significantly increased cell migration and sprouting from microbeads. Moreover, additional treatment with Tβ4 promoted the endothelial differentiation potential of hASCs by up-regulating various angiogenic genes. To evaluate the in vivo effects of the Tβ4-hASCs combination on vessel recruitment, dorsal window chambers were transplanted, and the co-treated mice were found to have a significantly increased number of microvessel branches. Transplantation of hASCs in combination with Tβ4 was found to improve blood flow and attenuate limb or foot loss post-ischemia compared to transplantation with hASCs alone. Taken together, the therapeutic application of hASCs combined with Tβ4 could be effective in enhancing endothelial differentiation and vascularization for treating hindlimb ischemia.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with hindlimb ischemia, transplanting hASCs with Tβ4 improved blood flow compared with hASCs alone.

    In plain wordsIn mice with limb ischemia, stem cells plus Tβ4 improved blood flow more than stem cells alone.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseBlood flow after ischemia, hASCs plus Tβ4 versus hASCs alone
    Study

    mousen: not reported in source

    mouse ischemic hindlimb model
    Comparator
    hASCs alone
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Transplantation of hASCs in combination with Tβ4 was found to improve blood flow and attenuate limb or foot loss post-ischemia compared to transplantation with hASCs alone.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with hindlimb ischemia, transplanting hASCs with Tβ4 attenuated limb or foot loss compared with hASCs alone.

    In plain wordsIn mice with limb ischemia, stem cells plus Tβ4 reduced limb or foot loss more than stem cells alone.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseLimb or foot loss after ischemia, hASCs plus Tβ4 versus hASCs alone
    Study

    mousen: not reported in source

    mouse ischemic hindlimb model
    Comparator
    hASCs alone
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Transplantation of hASCs in combination with Tβ4 was found to improve blood flow and attenuate limb or foot loss post-ischemia compared to transplantation with hASCs alone.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  3. 03LabIn vitro (cells)Mechanism

    In human adipose-derived stem cells, exogenous Tβ4 enhanced endogenous TMSB4X mRNA expression.

    In plain wordsIn cells, adding Tβ4 increased the cells' own Tβ4 gene activity.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseTMSB4X mRNA expression in hASCs
    Study

    humann: not reported in source

    human adipose-derived stem cells (hASCs)
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We observed that exogenous treatment with Tβ4 enhanced endogenous TMSB4X mRNA expression
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  4. 04LabIn vitro (cells)Mechanism

    In human adipose-derived stem cells, Tβ4 promoted morphological changes, with increased cell length.

    In plain wordsIn cells, Tβ4 made human stem cells longer.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasehASC cell length
    Study

    humann: not reported in source

    human adipose-derived stem cells (hASCs)
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    promoted morphological changes (increased cell length) in hASCs.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  5. 05LabIn vitro (cells)Mechanism

    In human adipose-derived stem cells, Tβ4 induced the active state of the cells by up-regulating the PI3K/AKT/mTOR and MAPK/ERK pathways.

    In plain wordsIn cells, Tβ4 turned up signaling pathways that make human stem cells more active.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasePI3K/AKT/mTOR and MAPK/ERK pathway activity
    Study

    humann: not reported in source

    human adipose-derived stem cells (hASCs)
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, Tβ4 induced the active state of hASCs by up-regulating intracellular signaling pathways including the PI3K/AKT/mTOR and MAPK/ERK pathways.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  6. 06LabIn vitro (cells)Efficacy outcome

    In human adipose-derived stem cells, Tβ4 significantly increased cell migration.

    In plain wordsIn cells, Tβ4 increased the movement of human stem cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasehASC migration
    Study

    humann: not reported in source

    human adipose-derived stem cells (hASCs)
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Treatment with Tβ4 significantly increased cell migration and sprouting from microbeads.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  7. 07LabIn vitro (cells)Efficacy outcome

    In human adipose-derived stem cells, Tβ4 significantly increased sprouting from microbeads.

    In plain wordsIn cells, Tβ4 increased sprouting from stem cell-covered beads.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasehASC sprouting from microbeads
    Study

    humann: not reported in source

    human adipose-derived stem cells (hASCs)
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Treatment with Tβ4 significantly increased cell migration and sprouting from microbeads.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.
  8. 08LabIn vitro (cells)Efficacy outcome

    In human adipose-derived stem cells, additional Tβ4 treatment promoted endothelial differentiation potential by up-regulating various angiogenic genes.

    In plain wordsIn cells, Tβ4 helped human stem cells turn toward blood vessel cells and raised blood vessel growth genes.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseEndothelial differentiation and angiogenic gene expression
    Study

    humann: not reported in source

    human adipose-derived stem cells (hASCs)
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, additional treatment with Tβ4 promoted the endothelial differentiation potential of hASCs by up-regulating various angiogenic genes.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.

Studies that did not test the peptide alone1

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 09AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome

    In mice with dorsal window chambers, co-treatment with Tβ4 and hASCs significantly increased the number of microvessel branches.

    In plain wordsIn mice, Tβ4 combined with stem cells increased the number of small blood vessel branches.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseMicrovessel branches with Tβ4 plus hASCs
    Study

    mousen: not reported in source

    dorsal window chamber with hASCs and Tβ4
    Outcome group
    Angiogenesis · Cardiovascular

    Source sentences

    Summary
    To evaluate the in vivo effects of the Tβ4-hASCs combination on vessel recruitment, dorsal window chambers were transplanted, and the co-treated mice were found to have a significantly increased number of microvessel branches.
    1 context sentence
    Summary · context
    In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse ischemic hindlimb model were investigated.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.