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Study · In vitro (cells) · mouse · 2020

10 results

HNGF6A Inhibits Oxidative Stress-Induced MC3T3-E1 Cell Apoptosis and Osteoblast Phenotype Inhibition by Targeting Circ_0001843/miR-214 Pathway.

Zhu X, Zhao Z, Zeng C, Chen B, Huang H, Chen Y, Zhou Q, Yang L, Lv J, Zhang J, Pan D, Shen J, Duque G, Cai D

Calcified tissue international18 Mar 2020Cited by 18 (Europe PMC)

PMID32189040 (opens in a new tab)DOI10.1007/s00223-020-00660-z (opens in a new tab)PepBankPUB-32189040
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    The humanin analogue HNGF6A exerted cytoprotection in MC3T3-E1 osteoblastic cells.

    In plain wordsIn mouse bone-cell lines, the humanin analogue HNGF6A protected cells from damage.

    Peptide
    Humanin
    Outcome
    IncreaseCytoprotection
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNGF6A, an analogue of HN, exerted cyto-protection and osteogenesis-promotion in MC3T3-E1 cells.
    1 context sentence
    Summary · context
    Cell proliferation of murine osteoblastic cell line MC3TC-E1 was examined by CCK8 assay and Edu staining.
  2. 02LabIn vitro (cells)Efficacy outcome

    HNGF6A promoted osteogenesis in MC3T3-E1 cells.

    In plain wordsIn mouse bone-cell lines, HNGF6A promoted bone-cell development.

    Peptide
    Humanin
    Outcome
    IncreaseOsteogenesis promotion
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNGF6A, an analogue of HN, exerted cyto-protection and osteogenesis-promotion in MC3T3-E1 cells.
    1 context sentence
    Summary · context
    Cell proliferation of murine osteoblastic cell line MC3TC-E1 was examined by CCK8 assay and Edu staining.
  3. 03LabIn vitro (cells)Efficacy outcome

    HNGF6A protected osteoblasts from oxidative stress-induced apoptosis.

    In plain wordsIn mouse bone-cell lines, HNGF6A protected cells from cell death caused by oxidative stress.

    Peptide
    Humanin
    Outcome
    DecreaseOxidative stress-induced apoptosis
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNGF6A protected osteoblasts from oxidative stress-induced apoptosis and osteoblast phenotype inhibition by targeting Circ_0001843/miR-214 pathway and the downstream kinases, p38 and JNK.
    1 context sentence
    Summary · context
    Cell apoptosis was detected by Annexin V assay under H2O2 treatment.
  4. 04LabIn vitro (cells)Mechanism

    HNGF6A significantly induced expression of osteoblast phenotype related proteins.

    In plain wordsIn mouse bone-cell lines, HNGF6A raised levels of proteins that mark bone-forming cells.

    Peptide
    Humanin
    Outcome
    IncreaseOsteoblast phenotype protein expression
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The expression of osteoblast phenotype related protein was significantly induced by HNGF6A.
    1 context sentence
    Summary · context
    We also tested the expression of osteoblast phenotype related protein by real-time PCR and Western blot.
  5. 05LabIn vitro (cells)Mechanism

    HNGF6A treatment decreased Circ_0001843 levels in MC3T3-E1 cells.

    In plain wordsIn mouse bone-cell lines, HNGF6A lowered levels of a circular RNA called Circ_0001843.

    Peptide
    Humanin
    Outcome
    DecreaseCirc_0001843 level
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Additionally, HNGF6A treatment decreased Circ_0001843 and increased miR-214 levels, as well as inhibited the phosphorylation of p38 and JNK.
  6. 06LabIn vitro (cells)Mechanism

    HNGF6A treatment increased miR-214 levels in MC3T3-E1 cells.

    In plain wordsIn mouse bone-cell lines, HNGF6A raised levels of miR-214.

    Peptide
    Humanin
    Outcome
    IncreasemiR-214 level
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Additionally, HNGF6A treatment decreased Circ_0001843 and increased miR-214 levels, as well as inhibited the phosphorylation of p38 and JNK.
  7. 07LabIn vitro (cells)Mechanism

    HNGF6A treatment inhibited phosphorylation of p38 in MC3T3-E1 cells.

    In plain wordsIn mouse bone-cell lines, HNGF6A reduced activation of the p38 kinase.

    Peptide
    Humanin
    Outcome
    Decreasep38 phosphorylation
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Additionally, HNGF6A treatment decreased Circ_0001843 and increased miR-214 levels, as well as inhibited the phosphorylation of p38 and JNK.
  8. 08LabIn vitro (cells)Mechanism

    HNGF6A treatment inhibited phosphorylation of JNK in MC3T3-E1 cells.

    In plain wordsIn mouse bone-cell lines, HNGF6A reduced activation of the JNK kinase.

    Peptide
    Humanin
    Outcome
    DecreaseJNK phosphorylation
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Additionally, HNGF6A treatment decreased Circ_0001843 and increased miR-214 levels, as well as inhibited the phosphorylation of p38 and JNK.

Studies that did not test the peptide alone2

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 09LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Circ_0001843 overexpression and miR-214 knockdown each significantly decreased the cytoprotection and osteogenic promotion seen with HNGF6A.

    In plain wordsIn mouse bone-cell lines, changing Circ_0001843 or miR-214 levels weakened the protective and bone-promoting effects of HNGF6A.

    Peptide
    Humanin
    Outcome
    DecreaseHNGF6A effects after Circ_0001843 overexpression or miR-214 knockdown
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal

    Source sentences

    Summary
    Furthermore, both Circ_0001843 overexpression and miR-214 knockdown significantly decreased the cyto-protection and osteogenic promotion of HNGF6A.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 10LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Circ_0001843 directly bound with miR-214, which in turn inhibited phosphorylation of p38 and JNK.

    In plain wordsIn mouse bone-cell lines, the circular RNA Circ_0001843 bound miR-214, which reduced p38 and JNK activation.

    Peptide
    Humanin
    Outcome
    Not applicableCirc_0001843 binding to miR-214
    Study

    mousen: not reported in source

    MC3T3-E1 murine osteoblastic cell line
    Outcome group
    Bone and joint · Musculoskeletal

    Source sentences

    Summary
    We further found that Circ_0001843 directly bound with miR-214, which in turn inhibited the phosphorylation of p38 and JNK.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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