Role for Kisspeptin and Neurokinin B in Regulation of Luteinizing Hormone and Testosterone Secretion in the Fetal Sheep.

Amodei R, Gribbin K, He W, Lindgren I, Corder KR, Jonker SS, Estill CT, Coolen LM, Lehman MN, Whitler W, Stormshak F, Roselli CE

Endocrinology1 Apr 2020Cited by 10 (Europe PMC)

PMID32005991PMCIDPMC7079722DOI10.1210/endocr/bqaa013PepBankPUB-32005991

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

3 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In anesthetized fetal lambs, intravenous kisspeptin‑10 elicited robust luteinizing hormone release.
In plain wordsIn fetal sheep, kisspeptin‑10 triggered a strong release of luteinizing hormone.
Peptide
Kisspeptin-10
Outcome
IncreaseLH release in anesthetized fetuses
Study
sheepn: not reported in source
Fetal lambs, gestational day 85
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated · intravenousAs given in this study (sheep).
Source sentences
Summary
In anesthetized fetuses, KP-10 elicited robust release of LH that was accompanied by a delayed rise in serum testosterone in males.
1 context sentence(design, population, supporting numbers)
Summary · context
We used GD85 fetal lambs to determine whether intravenous administration of kisspeptin-10 (KP-10) or senktide (NKB agonist) could elicit luteinizing hormone (LH) release.
Report a problemFND-3200599101
02AnimalAnimal study (in vivo)Efficacy outcome
In anesthetized male fetal lambs, kisspeptin‑10 was followed by a delayed rise in serum testosterone.
In plain wordsIn male fetal sheep, kisspeptin‑10 was followed by a delayed rise in testosterone.
Peptide
Kisspeptin-10
Outcome
IncreaseSerum testosterone in male anesthetized fetuses
Study
sheepn: not reported in source
Fetal lambs, gestational day 85
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated · intravenousAs given in this study (sheep).
Source sentences
Summary
In anesthetized fetuses, KP-10 elicited robust release of LH that was accompanied by a delayed rise in serum testosterone in males.
1 context sentence(design, population, supporting numbers)
Summary · context
We used GD85 fetal lambs to determine whether intravenous administration of kisspeptin-10 (KP-10) or senktide (NKB agonist) could elicit luteinizing hormone (LH) release.
Report a problemFND-3200599102
03AnimalAnimal study (in vivo)Efficacy outcome
In unanesthetized fetal lambs, kisspeptin‑10 elicited LH release.
In plain wordsIn fetal sheep that were awake, kisspeptin‑10 triggered release of luteinizing hormone.
Peptide
Kisspeptin-10
Outcome
IncreaseLH release in unanesthetized fetuses
Study
sheepn: not reported in source
Fetal lambs, gestational day 85
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated · intravenousAs given in this study (sheep).
Source sentences
Summary
In unanesthetized fetuses, senktide, as well as KP-10, elicited LH release.
1 context sentence(design, population, supporting numbers)
Summary · context
We used GD85 fetal lambs to determine whether intravenous administration of kisspeptin-10 (KP-10) or senktide (NKB agonist) could elicit luteinizing hormone (LH) release.
Report a problemFND-3200599104

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

04AnimalEndogenous levelAnimal study (in vivo)Other
In fetal lambs, kisspeptin-immunoreactive cells in the arcuate nucleus were more abundant in females than in males.
In plain wordsIn fetal sheep, the body's own kisspeptin-producing cells in the arcuate nucleus were more numerous in females than in males.
Peptide
Kisspeptin-10
Outcome
IncreaseArcuate kisspeptin-immunoreactive cells, sex difference
Study
sheepn: not reported in source
Fetal lambs, gestational day 60 and 85
Comparator
Males
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Kisspeptin and NKB immunoreactive cells in the arcuate nucleus were more abundant in females than in males.
1 context sentence(design, population, supporting numbers)
Summary · context
Immunohistochemistry and fluorescent in situ hybridization (FISH) were employed to localize these peptides in brains of GD60 and GD85 lamb fetuses.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3200599105
05AnimalEndogenous levelAnimal study (in vivo)Mechanism
In fetal lambs, more than 85% of arcuate kisspeptin cells costained for NKB.
In plain wordsIn fetal sheep, most of the body's own kisspeptin cells in the arcuate nucleus also contained neurokinin B.
Peptide
Kisspeptin-10
Outcome
Not applicableCo-staining of arcuate kisspeptin cells for NKB
Study
sheepn: not reported in source
Fetal lambs, gestational day 60 and 85
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Kisspeptin cells costained for NKB
> 85%
arcuate nucleus
Source sentences
Summary
Greater than 85% of arcuate kisspeptin cells costained for NKB.
1 context sentence(design, population, supporting numbers)
Summary · context
Immunohistochemistry and fluorescent in situ hybridization (FISH) were employed to localize these peptides in brains of GD60 and GD85 lamb fetuses.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3200599106
06AnimalEndogenous levelAnimal study (in vivo)Mechanism
In fetal lambs, FISH showed that most arcuate kisspeptin/NKB cells were kisspeptin/NKB/dynorphin (KNDy) neurons.
In plain wordsIn fetal sheep, most of the body's own kisspeptin and neurokinin B cells in the arcuate nucleus also made dynorphin.
Peptide
Kisspeptin-10
Outcome
Not applicableKNDy neuron identity of arcuate kisspeptin cells
Study
sheepn: not reported in source
Fetal lambs, gestational day 60 and 85
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
FISH revealed that the majority of these were kisspeptin/NKB/dynorphin (KNDy) neurons.
1 context sentence(design, population, supporting numbers)
Summary · context
Immunohistochemistry and fluorescent in situ hybridization (FISH) were employed to localize these peptides in brains of GD60 and GD85 lamb fetuses.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3200599107

Studies that did not test the peptide alone1

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

07AnimalNot a test of Kisspeptin-10 aloneAnimal study (in vivo)Mechanism
In anesthetized fetal lambs, pretreatment with the GnRH receptor antagonist acyline abolished the LH response to kisspeptin‑10.
In plain wordsIn fetal sheep, a drug that blocks the GnRH receptor stopped the LH rise that kisspeptin‑10 normally causes.
Peptide
Kisspeptin-10
Outcome
DecreaseLH response to kisspeptin-10 after GnRH receptor blockade
Study
sheepn: not reported in source
Fetal lambs, gestational day 85
Comparator
Kisspeptin-10 without acyline
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Pretreatment with the GnRH receptor antagonist (acyline) abolished the LH response to KP-10, confirming a hypothalamic site of action.
1 context sentence(design, population, supporting numbers)
Summary · context
We used GD85 fetal lambs to determine whether intravenous administration of kisspeptin-10 (KP-10) or senktide (NKB agonist) could elicit luteinizing hormone (LH) release.
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.
Report a problemFND-3200599103

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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