The Mitochondria-Derived Peptide Humanin Improves Recovery from Intracerebral Hemorrhage: Implication of Mitochondria Transfer and Microglia Phenotype Change.

Jung JE, Sun G, Bautista Garrido J, Obertas L, Mobley AS, Ting SM, Zhao X, Aronowski J

The Journal of neuroscience : the official journal of the Society for Neuroscience24 Jan 2020Cited by 68 (Europe PMC)

PMID31980585PMCIDPMC7055138DOI10.1523/jneurosci.2212-19.2020PepBankPUB-31980585

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study12

6 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Pharmacokinetics
In male mice with intracerebral hemorrhage, intravenously administered humanin penetrated perihematoma brain tissue.
In plain wordsIn mice with a brain bleed, humanin given into a vein reached brain tissue around the bleed.
Peptide
Humanin
Outcome
Not applicableBrain penetration of intravenous humanin
Study
micen: not reported in source
Intracerebral hemorrhage model in male mice
Outcome group
Pharmacokinetics · Nervous system and cognition
Studied dose
Dose not stated · intravenousAs given in this study (mice).
Source sentences
Summary
Intravenously administered HN penetrated perihematoma brain tissue, reduced neurological deficits, and improved hematoma clearance, a function that normally requires microglia/macrophages.
Report a problemFND-3198058510
02AnimalAnimal study (in vivo)Efficacy outcome
In male mice with intracerebral hemorrhage, intravenously administered humanin reduced neurological deficits.
In plain wordsIn mice with a brain bleed, humanin given into a vein reduced neurological problems.
Peptide
Humanin
Outcome
DecreaseNeurological deficits after ICH
Study
micen: not reported in source
Intracerebral hemorrhage model in male mice
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intravenousAs given in this study (mice).
Source sentences
Summary
Intravenously administered HN penetrated perihematoma brain tissue, reduced neurological deficits, and improved hematoma clearance, a function that normally requires microglia/macrophages.
Report a problemFND-3198058511
03AnimalAnimal study (in vivo)Efficacy outcome
In male mice with intracerebral hemorrhage, intravenously administered humanin improved hematoma clearance.
In plain wordsIn mice with a brain bleed, humanin given into a vein improved clearance of the blood clot.
Peptide
Humanin
Outcome
IncreaseHematoma clearance after ICH
Study
micen: not reported in source
Intracerebral hemorrhage model in male mice
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intravenousAs given in this study (mice).
Source sentences
Summary
Intravenously administered HN penetrated perihematoma brain tissue, reduced neurological deficits, and improved hematoma clearance, a function that normally requires microglia/macrophages.
Report a problemFND-3198058512
04LabIn vitro (cells)Mechanism
In cultured microglia, humanin alone upregulated expression of PPAR-gamma and its target genes, including mitochondrial superoxide dismutase.
In plain wordsIn cells, humanin raised a protective gene program in microglia.
Peptide
Humanin
Outcome
IncreasePPAR-gamma and target gene expression in microglia
Study
n: not reported in source
Microglia cell culture
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Similar to the effect of HN alone, incorporation of Mt by microglia
1 context sentence(design, population, supporting numbers)
Summary · context
(1) upregulated expression of the transcription factor peroxisome proliferator-activated receptor gamma and its target genes (including mitochondrial superoxide dismutase)
Report a problemFND-3198058503
05LabIn vitro (cells)Efficacy outcome
In cultured microglia, humanin alone enhanced phagocytic activity toward red blood cells.
In plain wordsIn cells, humanin made microglia clear red blood cells better.
Peptide
Humanin
Outcome
IncreaseMicroglial phagocytosis of red blood cells
Study
n: not reported in source
Microglia cell culture, in vitro hematoma clearance model
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Similar to the effect of HN alone, incorporation of Mt by microglia
1 context sentence(design, population, supporting numbers)
Summary · context
(2) enhanced phagocytic activity toward red blood cells
Report a problemFND-3198058504
06LabIn vitro (cells)Efficacy outcome
In cultured microglia, humanin alone reduced proinflammatory responses.
In plain wordsIn cells, humanin lowered inflammatory responses in microglia.
Peptide
Humanin
Outcome
DecreaseMicroglial proinflammatory responses
Study
n: not reported in source
Microglia cell culture
Outcome group
Inflammation markers · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Similar to the effect of HN alone, incorporation of Mt by microglia
1 context sentence(design, population, supporting numbers)
Summary · context
(3) reduced proinflammatory responses.
Report a problemFND-3198058505

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

07AnimalEndogenous levelAnimal study (in vivo)Other
In male mice, induction of intracerebral hemorrhage caused profound loss of the body's own humanin in the affected hemisphere.
In plain wordsIn male mice, a brain bleed sharply lowered the body's own humanin in the affected side of the brain.
Peptide
Humanin
Outcome
DecreaseHumanin in affected hemisphere after ICH
Study
micen: not reported in source
Intracerebral hemorrhage model in male mice
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
ICH induction in male mice caused profound HN loss in the affected hemisphere.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3198058509
08LabEndogenous levelIn vitro (cells)Other
In cell culture, astrocytes released humanin together with intact mitochondria.
In plain wordsIn cells, astrocytes released the body's own humanin along with mitochondria.
Peptide
Humanin
Outcome
IncreaseAstrocyte release of humanin
Study
n: not reported in source
Astrocyte cell culture
Outcome group
Mitochondrial function · Nervous system and cognition
Source sentences
Summary
Using a cell culture system, we established that astrocytes release both intact Mt and humanin (HN), a small bioactive peptide normally transcribed from the Mt genome.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3198058501
09LabEndogenous levelIn vitro (cells)Other
In cell culture, astrocyte-secreted mitochondria entered microglia and induced humanin expression.
In plain wordsIn cells, mitochondria from astrocytes entered microglia and raised the body's own humanin there.
Peptide
Humanin
Outcome
IncreaseHumanin expression in microglia after astrocyte mitochondria uptake
Study
n: not reported in source
Microglia cell culture
Outcome group
Mitochondrial function · Nervous system and cognition
Source sentences
Summary
Further experiments revealed that astrocyte-secreted Mt enter microglia, where they induce HN expression.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3198058502

Studies that did not test the peptide alone3

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

10LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In cultured microglia, incorporation of astrocyte mitochondria upregulated PPAR-gamma and its target genes, including mitochondrial superoxide dismutase.
In plain wordsIn cells, taking in astrocyte mitochondria raised a protective gene program in microglia.
Peptide
Humanin
Outcome
IncreasePPAR-gamma and target gene expression after mitochondria uptake
Study
n: not reported in source
Microglia cell culture
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Source sentences
Summary
Similar to the effect of HN alone, incorporation of Mt by microglia
1 context sentence(design, population, supporting numbers)
Summary · context
(1) upregulated expression of the transcription factor peroxisome proliferator-activated receptor gamma and its target genes (including mitochondrial superoxide dismutase)
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3198058506
11LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
In cultured microglia, incorporation of astrocyte mitochondria enhanced phagocytic activity toward red blood cells.
In plain wordsIn cells, taking in astrocyte mitochondria made microglia clear red blood cells better.
Peptide
Humanin
Outcome
IncreaseMicroglial phagocytosis after mitochondria uptake
Study
n: not reported in source
Microglia cell culture, in vitro hematoma clearance model
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Similar to the effect of HN alone, incorporation of Mt by microglia
1 context sentence(design, population, supporting numbers)
Summary · context
(2) enhanced phagocytic activity toward red blood cells
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3198058507
12LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
In cultured microglia, incorporation of astrocyte mitochondria reduced proinflammatory responses.
In plain wordsIn cells, taking in astrocyte mitochondria lowered inflammatory responses in microglia.
Peptide
Humanin
Outcome
DecreaseMicroglial proinflammatory responses after mitochondria uptake
Study
n: not reported in source
Microglia cell culture
Outcome group
Inflammation markers · Nervous system and cognition
Source sentences
Summary
Similar to the effect of HN alone, incorporation of Mt by microglia
1 context sentence(design, population, supporting numbers)
Summary · context
(3) reduced proinflammatory responses.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3198058508

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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