Recombinant B2L and Kisspeptin-54 DNA Vaccine Induces Immunity Against Orf Virus and Inhibits Spermatogenesis In Rats.

Wassie T, Fanmei Z, Jiang X, Liu G, Girmay S, Min Z, Chenhui L, Bo DD, Ahmed S

Scientific reports7 Nov 2019Cited by 21 (Europe PMC)

PMID31700161PMCIDPMC6838309DOI10.1038/s41598-019-52744-yPepBankPUB-31700161

Summary

Orf is a highly contagious zoonotic disease of small ruminants caused by Parapoxvirus. Kisspeptin, encoded by the KISS1 gene with its cognate receptor GPR-54 is recognized as an upstream orchestrator in the hypothalamic-pituitary-gonadal axis. This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology. The administration of this recombinant vaccine is shown to produce a significant antibody and cell-mediated immune response directed against B2L compared to the control group (p < 0.05). Furthermore, we found that rats inoculated with PBK-asd vaccine up-regulated antigen-mediated splenocyte proliferation and significantly raised antigen-specific tumor necrosis factor-alpha (TNFα-), interferon-gamma (IFN-ϒ) and interleukin (IL-2) compared to the control group (p < 0.05). This recombinant vaccine also stimulated antibody responses to kisspeptin and decreased serum luteinizing hormone and testosterone levels. Moreover, the current recombinant vaccine caused testicular atrophy and arrested spermatogenesis. It is concluded that this recombinant B2L and Kisspeptin-54 vaccine could be a promising approach for construction of bivalent orf virus and immunocastration vaccine. Furthermore, we concluded that the orf virus envelope protein (B2L) could be used as an immunomodulator for kisspeptin-54 to produce a strong antibody response.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study11

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone11

In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-54 (metastin).

01AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine produced a significant antibody response against B2L versus control.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 produced an antibody response against the virus protein compared with control rats.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseAntibody response against B2L
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Comparator
Control group
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
The administration of this recombinant vaccine is shown to produce a significant antibody and cell-mediated immune response directed against B2L compared to the control group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016101
02AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine produced a significant cell-mediated immune response against B2L versus control.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 produced a cell-mediated immune response against the virus protein compared with control rats.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseCell-mediated immune response against B2L
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Comparator
Control group
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
The administration of this recombinant vaccine is shown to produce a significant antibody and cell-mediated immune response directed against B2L compared to the control group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016102
03AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine up-regulated antigen-mediated splenocyte proliferation versus control.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 increased immune cell growth in the spleen compared with control rats.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseAntigen-mediated splenocyte proliferation
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Comparator
Control group
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
Furthermore, we found that rats inoculated with PBK-asd vaccine up-regulated antigen-mediated splenocyte proliferation and significantly raised antigen-specific tumor necrosis factor-alpha (TNFα-), interferon-gamma (IFN-ϒ) and interleukin (IL-2) compared to the control group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016103
04AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine raised antigen-specific TNF-alpha versus control.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 raised the immune signal TNF-alpha compared with control rats.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseAntigen-specific TNF-alpha
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Comparator
Control group
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
Furthermore, we found that rats inoculated with PBK-asd vaccine up-regulated antigen-mediated splenocyte proliferation and significantly raised antigen-specific tumor necrosis factor-alpha (TNFα-), interferon-gamma (IFN-ϒ) and interleukin (IL-2) compared to the control group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016104
05AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine raised antigen-specific interferon-gamma versus control.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 raised the immune signal interferon-gamma compared with control rats.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseAntigen-specific interferon-gamma
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Comparator
Control group
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
Furthermore, we found that rats inoculated with PBK-asd vaccine up-regulated antigen-mediated splenocyte proliferation and significantly raised antigen-specific tumor necrosis factor-alpha (TNFα-), interferon-gamma (IFN-ϒ) and interleukin (IL-2) compared to the control group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016105
06AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine raised antigen-specific interleukin 2 versus control.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 raised the immune signal interleukin 2 compared with control rats.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseAntigen-specific interleukin 2
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Comparator
Control group
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
Furthermore, we found that rats inoculated with PBK-asd vaccine up-regulated antigen-mediated splenocyte proliferation and significantly raised antigen-specific tumor necrosis factor-alpha (TNFα-), interferon-gamma (IFN-ϒ) and interleukin (IL-2) compared to the control group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016106
07AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine stimulated antibody responses to kisspeptin.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 caused the body to make antibodies against kisspeptin.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseAntibody response to kisspeptin
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
This recombinant vaccine also stimulated antibody responses to kisspeptin and decreased serum luteinizing hormone and testosterone levels.
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016107
08AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine decreased serum luteinizing hormone.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 lowered luteinizing hormone.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSerum luteinizing hormone
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
This recombinant vaccine also stimulated antibody responses to kisspeptin and decreased serum luteinizing hormone and testosterone levels.
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016108
09AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine decreased serum testosterone.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 lowered testosterone.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSerum testosterone
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
This recombinant vaccine also stimulated antibody responses to kisspeptin and decreased serum luteinizing hormone and testosterone levels.
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016109
10AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine caused testicular atrophy.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 caused the testes to shrink.
Peptide
Kisspeptin-54 (metastin)
Outcome
IncreaseTesticular atrophy
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Moreover, the current recombinant vaccine caused testicular atrophy and arrested spermatogenesis.
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016110
11AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rats, the recombinant B2L and kisspeptin‑54 DNA vaccine arrested spermatogenesis.
In plain wordsIn rats, a vaccine combining an orf virus protein and kisspeptin‑54 stopped sperm production.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSpermatogenesis
Study
ratn: not reported in source
Recombinant B2L and kisspeptin-54 DNA vaccine in rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Moreover, the current recombinant vaccine caused testicular atrophy and arrested spermatogenesis.
1 context sentence(design, population, supporting numbers)
Summary · context
This study was designed to construct a DNA vaccine that produces a fused peptide composed of a major immunodominant protein of the orf virus (B2L) and kisspeptin-54, a neuropeptide with recognized roles in mammalian reproductive biology.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Show in the study text Report a problemFND-3170016111

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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