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Study · In vitro (cells) · chinese hamster · 2019

5 results

Analyzing the signaling properties of gar (Lepisosteus oculatus) melanocortin receptors: Evaluating interactions with MRAP1 and MRAP2.

Wolverton EA, Wong MK, Davis PE, Hoglin B, Braasch I, Dores RM

General and comparative endocrinology2 Jul 2019Cited by 17 (Europe PMC)

PMID31276671 (opens in a new tab)PMCIDPMC7263024 (opens in a new tab)DOI10.1016/j.ygcen.2019.113215 (opens in a new tab)PepBankPUB-31276671
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In CHO cells, gar Mc2r co-expressed with gMrap1 showed only a low level of activation by NDP-MSH, at 10^-7 M and 10^-6 M.

    In plain wordsIn cells, the gar Mc2r receptor responded only weakly to NDP-MSH, and only at the two highest doses.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasegMc2r activation by NDP-MSH with gMrap1
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing gar melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Furthermore, co-expression of gMc2r with gMrap1 followed by stimulation with NDP-MSH resulted in a low level of activation (only at 10-7 M and 10-6 M).
    1 context sentence
    Summary · context
    Against this background, an objective of this study was to determine whether the ligand sensitivity for either ACTH or α-MSH was affected when gar (g) melanocortin receptors (Mcrs) were co-expressed with either of the accessory proteins gMrap1 or gMrap2 in Chinese Hamster Ovary cells.
  2. 02LabIn vitro (cells)Activity measurement

    In CHO cells, gar Mc1r, Mc3r, Mc4r and Mc5r responded more robustly to NDP-MSH than gMc2r did.

    In plain wordsIn cells, four gar melanocortin receptors responded to NDP-MSH more strongly than the gar Mc2r receptor.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasegMc1r, gMc3r, gMc4r and gMc5r response to NDP-MSH
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing gar melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, gMc1r, gMc3r, gMc4r, and gMc5r responded to stimulation by NDP-MSH in a more robust manner.
    1 context sentence
    Summary · context
    Against this background, an objective of this study was to determine whether the ligand sensitivity for either ACTH or α-MSH was affected when gar (g) melanocortin receptors (Mcrs) were co-expressed with either of the accessory proteins gMrap1 or gMrap2 in Chinese Hamster Ovary cells.
  3. 03LabIn vitro (cells)Activity measurement

    In CHO cells, co-expression of gMRAP1 with gMc1r, gMc3r, gMc4r or gMc5r did not change sensitivity to NDP-MSH.

    In plain wordsIn cells, adding the accessory protein gMRAP1 did not change how sensitive four gar receptors were to NDP-MSH.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeEffect of gMRAP1 co-expression on NDP-MSH sensitivity
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing gar melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Co-expression of gMc1r, gMc3r, gMc4r, and gMc5r with gMRAP1 had no effect on sensitivity to stimulation by NDP-MSH or hACTH(1-24).
    1 context sentence
    Summary · context
    Against this background, an objective of this study was to determine whether the ligand sensitivity for either ACTH or α-MSH was affected when gar (g) melanocortin receptors (Mcrs) were co-expressed with either of the accessory proteins gMrap1 or gMrap2 in Chinese Hamster Ovary cells.
  4. 04LabIn vitro (cells)Activity measurement

    In CHO cells, co-expression of gMRAP2 had no negative or positive effect on ligand sensitivity of gMc1r, gMc3r and gMc5r.

    In plain wordsIn cells, adding the accessory protein gMRAP2 did not change how sensitive three gar receptors were to their ligands.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeEffect of gMRAP2 co-expression on ligand sensitivity of gMc1r, gMc3r and gMc5r
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing gar melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Co-expression with gMRAP2 had no negative or positive effect on ligand sensitivity for gMc1r, gMc3r, and gMc5r
    1 context sentence
    Summary · context
    Against this background, an objective of this study was to determine whether the ligand sensitivity for either ACTH or α-MSH was affected when gar (g) melanocortin receptors (Mcrs) were co-expressed with either of the accessory proteins gMrap1 or gMrap2 in Chinese Hamster Ovary cells.
  5. 05LabIn vitro (cells)Activity measurement

    In CHO cells, co-expression of gMRAP2 increased activation of gMc4r by NDP-MSH.

    In plain wordsIn cells, adding the accessory protein gMRAP2 made the gar Mc4r receptor respond more strongly to NDP-MSH.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasegMc4r activation by NDP-MSH with gMRAP2
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing gar melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    however this treatment did increase the activation of CHO cells transfected with gMc4r following stimulation with both hACTH(1-24) (p < 0.001), and NDP-MSH (p < 0.001).
    1 context sentence
    Summary · context
    Against this background, an objective of this study was to determine whether the ligand sensitivity for either ACTH or α-MSH was affected when gar (g) melanocortin receptors (Mcrs) were co-expressed with either of the accessory proteins gMrap1 or gMrap2 in Chinese Hamster Ovary cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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