Skip to content

Study · Animal study (in vivo) · mouse · 2019

9 results

Impacts of obesity, maternal obesity and nicotinamide mononucleotide supplementation on sperm quality in mice.

Youngson NA, Uddin GM, Das A, Martinez C, Connaughton HS, Whiting S, Yu J, Sinclair DA, Aitken RJ, Morris MJ

Reproduction (Cambridge, England)1 Aug 2019Cited by 29 (Europe PMC)

PMID31226694 (opens in a new tab)PMCIDPMC6589912 (opens in a new tab)DOI10.1530/rep-18-0574 (opens in a new tab)PepBankPUB-31226694
Download PDFPubMedAsk about this study
Jump to 9 results

Summary

Male fertility and sperm quality are negatively impacted by obesity. Furthermore, recent evidence has shown that male offspring from obese rat mothers also have reduced sperm quality and fertility. Here, we extend work in this area by comparing the effects of both maternal obesity and offspring post-weaning diet-induced obesity, as well as their combination, on sperm quality in mice. We additionally tested whether administration of the NAD+-booster nicotinamide mononucleotide (NMN) can ameliorate the negative effects of obesity and maternal obesity on sperm quality. We previously showed that intraperitoneal (i.p.) injection of NMN can reduce the metabolic deficits induced by maternal obesity or post-weaning dietary obesity in mice. In this study, female mice were fed a high-fat diet (HFD) for 6 weeks until they were 18% heavier than a control diet group. Thereafter, HFD and control female mice were mated with control diet males, and male offspring were weaned into groups receiving control or HFD. At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days. As expected, adiposity was increased by both maternal and post-weaning HFD but reduced by NMN supplementation. Post-weaning HFD reduced sperm count and motility, while maternal HFD increased offspring sperm DNA fragmentation and levels of aberrant sperm chromatin. There was no evidence that the combination of post-weaning and maternal HFD exacerbated the impacts in sperm quality suggesting that they impact spermatogenesis through different mechanisms. Surprisingly NMN reduced sperm count, vitality and increased sperm oxidative DNA damage, which was associated with increased NAD+ in testes. A subsequent experiment using oral NMN at 400 mg/kg body weight was not associated with reduced sperm viability, oxidative stress, mitochondrial dysfunction or increased NAD+ in testes, suggesting that the negative impacts on sperm could be dependent on dose or mode of administration.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice, NMN reduced adiposity associated with maternal and post-weaning high-fat diets versus vehicle.

    In plain wordsIn mice, injected NMN reduced body fat versus vehicle.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseAdiposity
    Study

    mousen: not reported in source

    Maternal and post-weaning dietary obesity
    Comparator
    Vehicle PBS
    Outcome group
    Body weight · Metabolic and weight
    Studied dose
    500 mg/kg body weight · intraperitoneal · 21 daysAs given in this study (mouse).

    Source sentences

    Summary
    As expected, adiposity was increased by both maternal and post-weaning HFD but reduced by NMN supplementation.
    1 context sentence
    Summary · context
    At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days.
  2. 02AnimalAnimal study (in vivo)Safety outcome

    In mice, injected NMN reduced sperm count versus vehicle.

    In plain wordsIn mice, injected NMN reduced sperm count versus vehicle.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseSperm count
    Study

    mousen: not reported in source

    Maternal and post-weaning dietary obesity
    Comparator
    Vehicle PBS
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    500 mg/kg body weight · intraperitoneal · 21 daysAs given in this study (mouse).

    Source sentences

    Summary
    Surprisingly NMN reduced sperm count, vitality and increased sperm oxidative DNA damage, which was associated with increased NAD+ in testes.
    1 context sentence
    Summary · context
    At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days.
  3. 03AnimalAnimal study (in vivo)Safety outcome

    In mice, injected NMN reduced sperm vitality versus vehicle.

    In plain wordsIn mice, injected NMN reduced sperm vitality versus vehicle.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseSperm vitality
    Study

    mousen: not reported in source

    Maternal and post-weaning dietary obesity
    Comparator
    Vehicle PBS
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    500 mg/kg body weight · intraperitoneal · 21 daysAs given in this study (mouse).

    Source sentences

    Summary
    Surprisingly NMN reduced sperm count, vitality and increased sperm oxidative DNA damage, which was associated with increased NAD+ in testes.
    1 context sentence
    Summary · context
    At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days.
  4. 04AnimalAnimal study (in vivo)Safety outcome

    In mice, injected NMN increased sperm oxidative DNA damage versus vehicle.

    In plain wordsIn mice, injected NMN increased oxidative damage to sperm DNA versus vehicle.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseSperm oxidative DNA damage
    Study

    mousen: not reported in source

    Maternal and post-weaning dietary obesity
    Comparator
    Vehicle PBS
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    500 mg/kg body weight · intraperitoneal · 21 daysAs given in this study (mouse).

    Source sentences

    Summary
    Surprisingly NMN reduced sperm count, vitality and increased sperm oxidative DNA damage, which was associated with increased NAD+ in testes.
    1 context sentence
    Summary · context
    At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In mice, injected NMN-associated sperm changes accompanied increased testicular NAD+.

    In plain wordsIn mice, sperm changes after injected NMN accompanied increased NAD+ in the testes.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseTesticular NAD+
    Study

    mousen: not reported in source

    Maternal and post-weaning dietary obesity
    Comparator
    Vehicle PBS
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    500 mg/kg body weight · intraperitoneal · 21 daysAs given in this study (mouse).

    Source sentences

    Summary
    Surprisingly NMN reduced sperm count, vitality and increased sperm oxidative DNA damage, which was associated with increased NAD+ in testes.
    1 context sentence
    Summary · context
    At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days.
  6. 06AnimalAnimal study (in vivo)Safety outcome

    In mice, oral NMN was not associated with reduced sperm viability.

    In plain wordsIn mice, oral NMN was not linked to reduced sperm viability.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeSperm viability
    Study

    mousen: not reported in source

    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    400 mg/kg body weight · oralAs given in this study (mouse).

    Source sentences

    Summary
    A subsequent experiment using oral NMN at 400 mg/kg body weight was not associated with reduced sperm viability, oxidative stress, mitochondrial dysfunction or increased NAD+ in testes, suggesting that the negative impacts on sperm could be dependent on dose or mode of administration.
  7. 07AnimalAnimal study (in vivo)Safety outcome

    In mice, oral NMN was not associated with sperm oxidative stress.

    In plain wordsIn mice, oral NMN was not linked to sperm oxidative stress.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeSperm oxidative stress
    Study

    mousen: not reported in source

    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    400 mg/kg body weight · oralAs given in this study (mouse).

    Source sentences

    Summary
    A subsequent experiment using oral NMN at 400 mg/kg body weight was not associated with reduced sperm viability, oxidative stress, mitochondrial dysfunction or increased NAD+ in testes, suggesting that the negative impacts on sperm could be dependent on dose or mode of administration.
  8. 08AnimalAnimal study (in vivo)Safety outcome

    In mice, oral NMN was not associated with sperm mitochondrial dysfunction.

    In plain wordsIn mice, oral NMN was not linked to impaired sperm mitochondrial function.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeSperm mitochondrial dysfunction
    Study

    mousen: not reported in source

    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    400 mg/kg body weight · oralAs given in this study (mouse).

    Source sentences

    Summary
    A subsequent experiment using oral NMN at 400 mg/kg body weight was not associated with reduced sperm viability, oxidative stress, mitochondrial dysfunction or increased NAD+ in testes, suggesting that the negative impacts on sperm could be dependent on dose or mode of administration.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In mice, oral NMN was not associated with increased testicular NAD+.

    In plain wordsIn mice, oral NMN was not linked to increased NAD+ in the testes.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeTesticular NAD+ after oral NMN
    Study

    mousen: not reported in source

    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    400 mg/kg body weight · oralAs given in this study (mouse).

    Source sentences

    Summary
    A subsequent experiment using oral NMN at 400 mg/kg body weight was not associated with reduced sperm viability, oxidative stress, mitochondrial dysfunction or increased NAD+ in testes, suggesting that the negative impacts on sperm could be dependent on dose or mode of administration.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.