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Study · Ex vivo tissue · 2019

7 results

Potent humanin analogue (HNG) protects human sperm from freeze-thaw-induced damage.

Yang C, Xu L, Cui Y, Wu B, Liao Z

Cryobiology6 Apr 2019Cited by 20 (Europe PMC)

PMID30959025 (opens in a new tab)DOI10.1016/j.cryobiol.2019.04.001 (opens in a new tab)PepBankPUB-30959025
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In human sperm from healthy men, HNG at 2, 10 and 20 μM in freezing media improved post-thaw sperm motility versus control.

    In plain wordsIn lab tests on human sperm, adding HNG to freezing media improved sperm movement after thawing, compared with media without HNG.

    Peptide
    Humanin
    Outcome
    IncreaseSperm motility after freeze-thaw
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    Control, freezing media without HNG
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    2, 10 and 20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    As a result, supplementation of HNG with 2 μM, 10 μM and 20 μM to the freezing media all significantly improved sperm motility and viability (all p < 0.05) when compared with the control group.
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).
  2. 02LabEx vivo tissueEfficacy outcome

    In human sperm from healthy men, HNG at 2, 10 and 20 μM in freezing media improved post-thaw sperm viability versus control.

    In plain wordsIn lab tests on human sperm, adding HNG to freezing media improved sperm survival after thawing, compared with media without HNG.

    Peptide
    Humanin
    Outcome
    IncreaseSperm viability after freeze-thaw
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    Control, freezing media without HNG
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    2, 10 and 20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    As a result, supplementation of HNG with 2 μM, 10 μM and 20 μM to the freezing media all significantly improved sperm motility and viability (all p < 0.05) when compared with the control group.
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).
  3. 03LabEx vivo tissueEfficacy outcome

    In frozen-thawed human sperm, HNG supplementation reduced damage to the mitochondrial membrane.

    In plain wordsIn lab tests on human sperm, HNG in the freezing media reduced damage to sperm mitochondrial membranes.

    Peptide
    Humanin
    Outcome
    DecreaseSperm mitochondrial membrane damage
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    Control, freezing media without HNG
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    2, 10 and 20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    supplementation of HNG reduced the damage to the mitochondrial membrane and DNA integrity, and inhibited the reaction of oxidative stress and the activity of caspase-3 in sperm.
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).
  4. 04LabEx vivo tissueEfficacy outcome

    In frozen-thawed human sperm, HNG supplementation reduced damage to DNA integrity.

    In plain wordsIn lab tests on human sperm, HNG in the freezing media reduced DNA damage in sperm.

    Peptide
    Humanin
    Outcome
    IncreaseSperm DNA integrity
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    Control, freezing media without HNG
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    2, 10 and 20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    supplementation of HNG reduced the damage to the mitochondrial membrane and DNA integrity, and inhibited the reaction of oxidative stress and the activity of caspase-3 in sperm.
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).
  5. 05LabEx vivo tissueEfficacy outcome

    In frozen-thawed human sperm, HNG supplementation inhibited the oxidative stress reaction.

    In plain wordsIn lab tests on human sperm, HNG in the freezing media reduced oxidative stress in sperm.

    Peptide
    Humanin
    Outcome
    DecreaseSperm oxidative stress
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    Control, freezing media without HNG
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    2, 10 and 20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    inhibited the reaction of oxidative stress and the activity of caspase-3 in sperm.
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).
  6. 06LabEx vivo tissueMechanism

    In frozen-thawed human sperm, HNG supplementation inhibited caspase-3 activity.

    In plain wordsIn lab tests on human sperm, HNG in the freezing media lowered the activity of caspase-3, a cell-death enzyme.

    Peptide
    Humanin
    Outcome
    DecreaseSperm caspase-3 activity
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    Control, freezing media without HNG
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    2, 10 and 20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    the activity of caspase-3 in sperm.
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).
  7. 07LabEx vivo tissueEfficacy outcome

    In frozen-thawed human sperm, HNG at 20 μM gave no significant improvement over 10 μM, although protective effects increased with concentration.

    In plain wordsIn lab tests on human sperm, 20 μM HNG did not work significantly better than 10 μM HNG.

    Peptide
    Humanin
    Outcome
    No significant change20 μM versus 10 μM HNG on sperm protective effects
    Study

    humann = 15

    Human sperm from healthy men, frozen and thawed
    Comparator
    HNG 10 μM
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    20 μM · added to freezing mediaAs given in this study (human).

    Source sentences

    Summary
    Although these protective effects increased with the elevated concentration of HNG in the freezing media, a final HNG concentration of 20 μM failed to exert significant improvements when compared with the concentration of 10 μM (all p > 0.05).
    2 context sentences
    Summary · context
    We collected semen samples with normal sperm parameters from 15 healthy men.
    Summary · context
    In the other three groups, the spermatozoa were frozen in media supplemented with different concentrations of HNG (2 μM, 10 μM and 20 μM, respectively).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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