Maternal high-fat diet impairs follicular development of offspring through intraovarian kisspeptin/GPR54 system.

Zhou Z, Lin Q, Xu X, Illahi GS, Dong C, Wu X

Reproductive biology and endocrinology : RB&E22 Jan 2019Cited by 25 (Europe PMC)

PMID30670046PMCIDPMC6343291DOI10.1186/s12958-019-0457-zPepBankPUB-30670046

Summary

BackgroundExcessive gestational weight gain (GWG), which is associated with adverse long-term effects on the health of the offspring, has become a major clinical problem. Accumulating evidence indicates that the ovary kisspeptin/GPR54 system directly participates in a series of physiological activities. We used a model of high-fat diet (HFD) during gestational to investigate offspring's ovarian function and whether kisspeptin/GPR54 system is involved.MethodsAfter introducing the male and confirmation of mating by checking a vaginal sperm plug, female rats were randomized into two groups: control diet called NCD group and high-fat diet called HFD group. After birth, all rats were changed into a control diet and litter size was adjusted to 12 pups per litter. Ovaries were collected for assessment at postnatal day (PND) 4 and PND 30. The timing of vaginal opening was recorded, and the estrous cyclicity was monitored for 2 consecutive weeks immediately. Primary granulosa cells and ovaries which were taken from PND 4 were collected for determination of the direct effect of kisspeptin-10 (kp-10) in vitro.ResultsNeonatal rats exposed to HFD during gestation had a lower number of secondary follicles in the ovary. The expression of follicle-stimulating hormone receptor (FSHR) and kisspeptin was not altered. At prepuberty, the number of antral follicles and preovulatory follicles was elevated with decreased type III follicles in the HFD group. While the expression of ovulation-related genes was decreased, the expression levels of follicular growth-related genes and steroidogenesis synthesis related genes were elevated. A significant increase in kiss1 mRNA and kisspeptin protein was detected without changes in kiss1r mRNA and GPR54. Maternal high-fat diet during gestation resulted in a significant advanced puberty onset and an irregular estrous cycle in offspring rats. In addition, the administration of kp-10 produced an increase in viability of primary granulosa cells and enlarged the size of oocytes.ConclusionsHFD exposure during maternal gestation had a long-term effect on reproductive function in the offspring and the increased ovarian kisspeptin/GPR54 system might be involved.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

2 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In cultured rat ovaries, kp-10 enlarged the size of oocytes.
In plain wordsIn rat ovaries kept in the lab, kisspeptin‑10 made egg cells larger.
Peptide
Kisspeptin-10
Outcome
IncreaseOocyte size
Study
ratn: not reported in source
Ovaries from PND 4 rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
In addition, the administration of kp-10 produced an increase in viability of primary granulosa cells and enlarged the size of oocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
Primary granulosa cells and ovaries which were taken from PND 4 were collected for determination of the direct effect of kisspeptin-10 (kp-10) in vitro.
Show in the study text Report a problemFND-3067004605
02LabIn vitro (cells)Efficacy outcome
In primary rat granulosa cells, kp-10 increased cell viability.
In plain wordsIn rat ovary cells in the lab, kisspeptin‑10 increased cell viability.
Peptide
Kisspeptin-10
Outcome
IncreaseGranulosa cell viability
Study
ratn: not reported in source
Primary granulosa cells from PND 4 rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
In addition, the administration of kp-10 produced an increase in viability of primary granulosa cells and enlarged the size of oocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
Primary granulosa cells and ovaries which were taken from PND 4 were collected for determination of the direct effect of kisspeptin-10 (kp-10) in vitro.
Show in the study text Report a problemFND-3067004604

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

03AnimalEndogenous levelAnimal study (in vivo)Other
In neonatal rats exposed to a maternal high-fat diet, ovarian kisspeptin expression was not altered.
In plain wordsIn newborn rats whose mothers ate a high-fat diet, the body's own ovarian kisspeptin did not change.
Peptide
Kisspeptin-10
Outcome
No significant changeNeonatal ovarian kisspeptin expression after maternal HFD
Study
ratn: not reported in source
Offspring of dams fed a high-fat diet
Comparator
Offspring of control-diet dams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The expression of follicle-stimulating hormone receptor (FSHR) and kisspeptin was not altered.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal rats exposed to HFD during gestation had a lower number of secondary follicles in the ovary.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3067004601
04AnimalEndogenous levelAnimal study (in vivo)Other
In prepubertal rats exposed to a maternal high-fat diet, ovarian kiss1 mRNA increased significantly.
In plain wordsIn young rats whose mothers ate a high-fat diet, the ovary made more of the gene for the body's own kisspeptin.
Peptide
Kisspeptin-10
Outcome
IncreasePrepubertal kiss1 mRNA after maternal HFD
Study
ratn: not reported in source
Offspring of dams fed a high-fat diet
Comparator
Offspring of control-diet dams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
A significant increase in kiss1 mRNA and kisspeptin protein was detected without changes in kiss1r mRNA and GPR54.
1 context sentence(design, population, supporting numbers)
Summary · context
At prepuberty, the number of antral follicles and preovulatory follicles was elevated with decreased type III follicles in the HFD group.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3067004602
05AnimalEndogenous levelAnimal study (in vivo)Other
In prepubertal rats exposed to a maternal high-fat diet, ovarian kisspeptin protein increased significantly.
In plain wordsIn young rats whose mothers ate a high-fat diet, the body's own kisspeptin protein in the ovary was higher.
Peptide
Kisspeptin-10
Outcome
IncreasePrepubertal ovarian kisspeptin protein after maternal HFD
Study
ratn: not reported in source
Offspring of dams fed a high-fat diet
Comparator
Offspring of control-diet dams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
A significant increase in kiss1 mRNA and kisspeptin protein was detected without changes in kiss1r mRNA and GPR54.
1 context sentence(design, population, supporting numbers)
Summary · context
At prepuberty, the number of antral follicles and preovulatory follicles was elevated with decreased type III follicles in the HFD group.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3067004603

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

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