Effects of Kisspeptin-10 on Hypothalamic Neuropeptides and Neurotransmitters Involved in Appetite Control.

Orlando G, Leone S, Ferrante C, Chiavaroli A, Mollica A, Stefanucci A, Macedonio G, Dimmito MP, Leporini L, Menghini L, Brunetti L, Recinella L

Molecules (Basel, Switzerland)24 Nov 2018Cited by 35 (Europe PMC)

PMID30477219PMCIDPMC6321454DOI10.3390/molecules23123071PepBankPUB-30477219

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Summary

Besides its role as key regulator in gonadotropin releasing hormone secretion, reproductive function, and puberty onset, kisspeptin has been proposed to act as a bridge between energy homeostasis and reproduction. In the present study, to characterize the role of hypothalamic kisspeptin as metabolic regulator, we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells. Our study showed that kisspeptin-10 in the concentration range 1 nM⁻10 μM was well tolerated by the Hypo-E22 cell line. Moreover, kisspeptin-10 (100 nM⁻10 μM) concentration independently increased the gene expression of NPY while BDNF was inhibited only at the concentration of 10 μM. Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels. The inhibitory effect on DA and 5-HT is consistent with the increased peptide-induced DOPAC/DA and 5-HIIA/5-HT ratios. In conclusion, our current findings suggesting the increased NPY together with decreased BDNF and 5-HT activity following kisspeptin-10 would be consistent with a possible orexigenic effect induced by the peptide.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Safety outcome
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 from 1 nM to 10 µM was well tolerated.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 was well tolerated across the tested concentrations.
Peptide
Kisspeptin-10
Outcome
No significant changeCell tolerability
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Adverse events · Metabolic and weight
Studied dose
1 nM to 10 µMAs given in this study (rat).
Source sentences
Summary
Our study showed that kisspeptin-10 in the concentration range 1 nM⁻10 μM was well tolerated by the Hypo-E22 cell line.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721901
02LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 increased neuropeptide Y gene expression at 100 nM to 10 µM, independent of concentration.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 raised the gene activity of neuropeptide Y, an appetite signal.
Peptide
Kisspeptin-10
Outcome
IncreaseNPY gene expression
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
100 nM to 10 µMAs given in this study (rat).
Source sentences
Summary
Moreover, kisspeptin-10 (100 nM⁻10 μM) concentration independently increased the gene expression of NPY while BDNF was inhibited only at the concentration of 10 μM.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721902
03LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 inhibited BDNF gene expression only at 10 µM.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 lowered BDNF gene activity, but only at the highest concentration.
Peptide
Kisspeptin-10
Outcome
DecreaseBDNF gene expression
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
10 µMAs given in this study (rat).
Source sentences
Summary
Moreover, kisspeptin-10 (100 nM⁻10 μM) concentration independently increased the gene expression of NPY while BDNF was inhibited only at the concentration of 10 μM.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721903
04LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 decreased extracellular serotonin.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 lowered serotonin levels.
Peptide
Kisspeptin-10
Outcome
DecreaseExtracellular serotonin (5-HT)
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721904
05LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 decreased extracellular dopamine.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 lowered dopamine levels.
Peptide
Kisspeptin-10
Outcome
DecreaseExtracellular dopamine (DA)
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721905
06LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 left extracellular norepinephrine unaffected.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 did not change norepinephrine levels.
Peptide
Kisspeptin-10
Outcome
No significant changeExtracellular norepinephrine (NE)
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721906
07LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 increased the DOPAC/DA ratio.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 raised the ratio of a dopamine breakdown product to dopamine.
Peptide
Kisspeptin-10
Outcome
IncreaseDOPAC/DA ratio
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels. The inhibitory effect on DA and 5-HT is consistent with the increased peptide-induced DOPAC/DA and 5-HIIA/5-HT ratios.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721907
08LabIn vitro (cells)Mechanism
In rat hypothalamic Hypo-E22 cells, kisspeptin‑10 increased the 5-HIAA/5-HT ratio.
In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 raised the ratio of a serotonin breakdown product to serotonin.
Peptide
Kisspeptin-10
Outcome
Increase5-HIAA/5-HT ratio
Study
ratn: not reported in source
Hypo-E22 rat hypothalamic cell line
Outcome group
Appetite and food intake · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels. The inhibitory effect on DA and 5-HT is consistent with the increased peptide-induced DOPAC/DA and 5-HIIA/5-HT ratios.
1 context sentence(design, population, supporting numbers)
Summary · context
we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells.
Show in the study text Report a problemFND-3047721908

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

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PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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