Immunization against Kisspeptin-54 perturb hypothalamic-pituitary-testicular signaling pathway in ram lambs.

Wassie T, Liu G, Jiang X, Tesema B, Han Y, Zhao J, Girmay S, Ahmad HI, Ahmad HI

Theriogenology6 Nov 2018Cited by 17 (Europe PMC)

PMID30465983DOI10.1016/j.theriogenology.2018.10.029PepBankPUB-30465983

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study12

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone12

In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-54 (metastin).

01AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In ram lambs, immunization with a kisspeptin‑54 vaccine lowered serum kisspeptin versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered blood kisspeptin compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSerum kisspeptin
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Serum kisspeptin, luteinizing hormone and testosterone concentration in the treatment group was lower (p < 0.05) than that of the control group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598301
02AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In ram lambs, immunization with a kisspeptin‑54 vaccine lowered serum luteinizing hormone versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered luteinizing hormone compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSerum luteinizing hormone
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Serum kisspeptin, luteinizing hormone and testosterone concentration in the treatment group was lower (p < 0.05) than that of the control group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598302
03AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In ram lambs, immunization with a kisspeptin‑54 vaccine lowered serum testosterone versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered testosterone compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSerum testosterone
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Serum kisspeptin, luteinizing hormone and testosterone concentration in the treatment group was lower (p < 0.05) than that of the control group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598303
04AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine altered hypothalamic androgen receptor mRNA expression versus control; the direction is not stated.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine changed activity of the androgen receptor gene in the brain compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
Direction not reportedHypothalamic androgen receptor mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Compared with the control group, the mRNA expression of the hypothalamic androgen receptor (AR), KISS1, G protein-coupled receptor (GPR54) and gonadotropin-releasing hormone (GnRH) was altered in the immunized group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598304
05AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine altered hypothalamic KISS1 mRNA expression versus control; the direction is not stated.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine changed activity of the KISS1 gene in the brain compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
Direction not reportedHypothalamic KISS1 mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Compared with the control group, the mRNA expression of the hypothalamic androgen receptor (AR), KISS1, G protein-coupled receptor (GPR54) and gonadotropin-releasing hormone (GnRH) was altered in the immunized group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598305
06AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine altered hypothalamic GPR54 mRNA expression versus control; the direction is not stated.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine changed activity of the GPR54 receptor gene in the brain compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
Direction not reportedHypothalamic GPR54 mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Compared with the control group, the mRNA expression of the hypothalamic androgen receptor (AR), KISS1, G protein-coupled receptor (GPR54) and gonadotropin-releasing hormone (GnRH) was altered in the immunized group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598306
07AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine altered hypothalamic GnRH mRNA expression versus control; the direction is not stated.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine changed activity of the GnRH gene in the brain compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
Direction not reportedHypothalamic GnRH mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Compared with the control group, the mRNA expression of the hypothalamic androgen receptor (AR), KISS1, G protein-coupled receptor (GPR54) and gonadotropin-releasing hormone (GnRH) was altered in the immunized group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598307
08AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine lowered pituitary LHβ mRNA expression versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered activity of a luteinizing hormone gene in the pituitary compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreasePituitary LHβ mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Moreover, mRNA expression of pituitary luteinizing hormone beta (LHβ), follicle stimulating hormone beta (FSHβ), and GnRH receptor as well as, testicular LH receptor and FSH receptor, were remarkably lower (P < 0.05) in the treatment group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598308
09AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine lowered pituitary FSHβ mRNA expression versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered activity of a follicle stimulating hormone gene in the pituitary compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreasePituitary FSHβ mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Moreover, mRNA expression of pituitary luteinizing hormone beta (LHβ), follicle stimulating hormone beta (FSHβ), and GnRH receptor as well as, testicular LH receptor and FSH receptor, were remarkably lower (P < 0.05) in the treatment group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598309
10AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine lowered pituitary GnRH receptor mRNA expression versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered activity of the GnRH receptor gene in the pituitary compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreasePituitary GnRH receptor mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Source sentences
Summary
Moreover, mRNA expression of pituitary luteinizing hormone beta (LHβ), follicle stimulating hormone beta (FSHβ), and GnRH receptor as well as, testicular LH receptor and FSH receptor, were remarkably lower (P < 0.05) in the treatment group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598310
11AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine lowered testicular LH receptor mRNA expression versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered activity of the LH receptor gene in the testes compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseTesticular LH receptor mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Moreover, mRNA expression of pituitary luteinizing hormone beta (LHβ), follicle stimulating hormone beta (FSHβ), and GnRH receptor as well as, testicular LH receptor and FSH receptor, were remarkably lower (P < 0.05) in the treatment group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598311
12AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram lambs, the kisspeptin‑54 vaccine lowered testicular FSH receptor mRNA expression versus control.
In plain wordsIn ram lambs, a kisspeptin‑54 vaccine lowered activity of the FSH receptor gene in the testes compared with control lambs.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseTesticular FSH receptor mRNA
Study
sheep (ram lambs)n = 10
Kisspeptin-54 vaccine immunization
Comparator
Control group (empty plasmid)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Moreover, mRNA expression of pituitary luteinizing hormone beta (LHβ), follicle stimulating hormone beta (FSHβ), and GnRH receptor as well as, testicular LH receptor and FSH receptor, were remarkably lower (P < 0.05) in the treatment group.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3046598312

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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