Skip to content

Study · In vitro (cells) · 2018

7 results

Humanin Attenuates NMDA-Induced Excitotoxicity by Inhibiting ROS-dependent JNK/p38 MAPK Pathway.

Yang X, Zhang H, Wu J, Yin L, Yan LJ, Zhang C

International journal of molecular sciences29 Sept 2018Cited by 35 (Europe PMC)

PMID30274308 (opens in a new tab)PMCIDPMC6213259 (opens in a new tab)DOI10.3390/ijms19102982 (opens in a new tab)PepBankPUB-30274308
Download PDFPubMedAsk about this study
Jump to 7 results

Summary

Humanin (HN) is a novel 24-amino acid peptide that protects neurons against N-methyl-d-aspartate (NMDA)-induced toxicity. However, the contribution of the different mitogen-activated protein kinases (MAPKs) signals to HN neuroprotection against NMDA neurotoxicity remains unclear. The present study was therefore aimed to investigate neuroprotective mechanisms of HN. We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model. Results showed that: (1) HN attenuated NMDA-induced neuronal insults by increasing cell viability, decreasing lactate dehydrogenase (LDH) release, and increasing cell survival; (2) HN reversed NMDA-induced increase in intracellular calcium; (3) pretreatment by HN or 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA-AM), an intracellular calcium chelator, decreased ROS generation after NMDA exposure; (4) administration of HN or N-Acetyl-l-cysteine (NAC), a ROS scavenger, inhibited NMDA-induced JNK and p38 MAPK activation. These results indicated that HN reduced intracellular elevation of Ca2+ levels, which, in turn, inhibited ROS generation and subsequent JNK and p38 MAPK activation that are involved in promoting cell survival in NMDA-induced excitotoxicity. Therefore, the present study suggests that inhibition of ROS-dependent JNK/p38 MAPK signaling pathway serves an effective strategy for HN neuroprotection against certain neurological diseases.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cortical neurons exposed to NMDA, HN attenuated neuronal insults by increasing cell viability.

    In plain wordsIn nerve cells in the lab, HN protected against NMDA damage and raised cell viability.

    Peptide
    Humanin
    Outcome
    IncreaseCell viability after NMDA exposure
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN attenuated NMDA-induced neuronal insults by increasing cell viability
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.
  2. 02LabIn vitro (cells)Efficacy outcome

    In cortical neurons exposed to NMDA, HN decreased lactate dehydrogenase (LDH) release.

    In plain wordsIn nerve cells in the lab, HN lowered the release of LDH, a marker of cell damage, after NMDA exposure.

    Peptide
    Humanin
    Outcome
    DecreaseLDH release after NMDA exposure
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    decreasing lactate dehydrogenase (LDH) release
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.
  3. 03LabIn vitro (cells)Efficacy outcome

    In cortical neurons exposed to NMDA, HN increased cell survival.

    In plain wordsIn nerve cells in the lab, HN raised cell survival after NMDA exposure.

    Peptide
    Humanin
    Outcome
    IncreaseCell survival after NMDA exposure
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and increasing cell survival
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.
  4. 04LabIn vitro (cells)Mechanism

    In cortical neurons exposed to NMDA, HN reversed the NMDA-induced increase in intracellular calcium.

    In plain wordsIn nerve cells in the lab, HN reversed the rise in calcium inside cells caused by NMDA.

    Peptide
    Humanin
    Outcome
    DecreaseNMDA-induced intracellular calcium increase
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN reversed NMDA-induced increase in intracellular calcium
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.
  5. 05LabIn vitro (cells)Mechanism

    In cortical neurons, pretreatment by HN decreased reactive oxygen species generation after NMDA exposure.

    In plain wordsIn nerve cells in the lab, HN given before NMDA lowered the production of reactive oxygen species.

    Peptide
    Humanin
    Outcome
    DecreaseROS generation after NMDA exposure
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    pretreatment by HN or 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA-AM), an intracellular calcium chelator, decreased ROS generation after NMDA exposure
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.
  6. 06LabIn vitro (cells)Mechanism

    In cortical neurons, administration of HN inhibited NMDA-induced JNK activation.

    In plain wordsIn nerve cells in the lab, HN blocked the activation of the JNK signal caused by NMDA.

    Peptide
    Humanin
    Outcome
    DecreaseNMDA-induced JNK activation
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    administration of HN or N-Acetyl-l-cysteine (NAC), a ROS scavenger, inhibited NMDA-induced JNK and p38 MAPK activation.
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.
  7. 07LabIn vitro (cells)Mechanism

    In cortical neurons, administration of HN inhibited NMDA-induced p38 MAPK activation.

    In plain wordsIn nerve cells in the lab, HN blocked the activation of the p38 MAPK signal caused by NMDA.

    Peptide
    Humanin
    Outcome
    DecreaseNMDA-induced p38 MAPK activation
    Study

    n: not reported in source

    NMDA-mediated excitotoxicity of cortical neurons
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    administration of HN or N-Acetyl-l-cysteine (NAC), a ROS scavenger, inhibited NMDA-induced JNK and p38 MAPK activation.
    1 context sentence
    Summary · context
    We analyzed intracellular Ca2+ levels, reactive oxygen species (ROS) production, and the MAPKs signal transduction cascade using an in vitro NMDA-mediated excitotoxicity of cortical neurons model.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.