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Study · In vitro (cells) · mouse · 2018

10 results

Thymosin β4 is associated with bone sialoprotein expression via ERK and Smad3 signaling pathways in MDPC-23 odontoblastic cells.

Choi BD, Lim HJ, Lee SY, Lee MH, Kil KS, Lim DS, Jeong SJ, Jeong MJ

International journal of molecular medicine7 Sept 2018Cited by 4 (Europe PMC)

PMID30226623 (opens in a new tab)DOI10.3892/ijmm.2018.3865 (opens in a new tab)PepBankPUB-30226623
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

9 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 increased bone sialoprotein mRNA and protein levels, and PD98059 or SIS3 inhibited this increase.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 raised bone sialoprotein levels, and two pathway blockers stopped this.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseBone sialoprotein mRNA and protein
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 increased BSP mRNA and protein levels in MDPC‑23 cells, but this was inhibited by PD98059 or SIS3 treatment.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  2. 02LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 increased phosphorylated ERK1/2, and PD98059 or SIS3 inhibited this effect.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 raised activated ERK1/2, and two pathway blockers stopped this.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasePhosphorylated ERK1/2
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 increased levels of phosphorylated (p) extracellular signal‑regulated kinase (ERK)1/2, pSmad3, pβ‑catenin, and runt‑related transcription factor 2 (Runx2) protein, but these effects were inhibited by PD98059 or SIS3.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  3. 03LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 increased phosphorylated Smad3, and PD98059 or SIS3 inhibited this effect.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 raised activated Smad3, and two pathway blockers stopped this.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasePhosphorylated Smad3
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 increased levels of phosphorylated (p) extracellular signal‑regulated kinase (ERK)1/2, pSmad3, pβ‑catenin, and runt‑related transcription factor 2 (Runx2) protein, but these effects were inhibited by PD98059 or SIS3.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  4. 04LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 increased phosphorylated beta-catenin, and PD98059 or SIS3 inhibited this effect.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 raised activated beta-catenin, and two pathway blockers stopped this.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasePhosphorylated beta-catenin
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 increased levels of phosphorylated (p) extracellular signal‑regulated kinase (ERK)1/2, pSmad3, pβ‑catenin, and runt‑related transcription factor 2 (Runx2) protein, but these effects were inhibited by PD98059 or SIS3.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  5. 05LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 increased Runx2 protein, and PD98059 or SIS3 inhibited this effect.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 raised Runx2 protein, and two pathway blockers stopped this.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseRunx2 protein
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 increased levels of phosphorylated (p) extracellular signal‑regulated kinase (ERK)1/2, pSmad3, pβ‑catenin, and runt‑related transcription factor 2 (Runx2) protein, but these effects were inhibited by PD98059 or SIS3.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  6. 06LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 induced nuclear translocation of Runx2.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 moved Runx2 into the cell nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNuclear translocation of Runx2
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 induced the nuclear translocation of Runx2 and pSmad3, while nuclear translocation of β‑catenin was decreased.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  7. 07LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 induced nuclear translocation of pSmad3.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 moved activated Smad3 into the cell nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNuclear translocation of phosphorylated Smad3
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 induced the nuclear translocation of Runx2 and pSmad3, while nuclear translocation of β‑catenin was decreased.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  8. 08LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 decreased nuclear translocation of beta-catenin.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 reduced beta-catenin movement into the cell nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseNuclear translocation of beta-catenin
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 induced the nuclear translocation of Runx2 and pSmad3, while nuclear translocation of β‑catenin was decreased.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…
  9. 09LabIn vitro (cells)Mechanism

    In MDPC-23 cells, thymosin beta‑4 significantly increased bone sialoprotein promoter activity, and PD98059 or SIS3 decreased this activity.

    In plain wordsIn mouse tooth-forming cells, thymosin beta‑4 raised bone sialoprotein gene activity, and two pathway blockers lowered it.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseBone sialoprotein promoter activity
    Study

    mousen: not reported in source

    MDPC-23 odontoblastic cells
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 significantly increased BSP promoter activity, which was decreased by PD98059 or SIS3 treatment.
    1 context sentence
    Summary · context
    The effect of Tβ4 signaling on BSP expression was investigated by reverse transcription polymerase chain reaction, western blot analysis, immunofluorescence and a luciferase reporter assay in the presence or absence of specific inhibitors of mitogen activated protein kinase kinase (PD98059) and moth…

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 10AnimalEndogenous levelAnimal study (in vivo)Mechanism

    In developing mouse teeth, the body's own thymosin beta‑4 mRNA in the odontoblast layer was highest at postnatal day 5.

    In plain wordsIn developing mouse teeth, the body's own thymosin beta‑4 mRNA peaked at postnatal day 5.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableThymosin beta-4 mRNA in odontoblast layer over time
    Study

    mousen: not reported in source

    Mouse tooth development
    Outcome group
    Bone and joint · Musculoskeletal

    Source sentences

    Summary
    The expression of Tβ4 mRNA in the odontoblast layer was highest at postnatal day 5, known as the advanced bell stage, when odontoblasts actively secrete dentin matrix proteins.
    1 context sentence
    Summary · context
    Expression and localization of Tβ4 mRNA was determined by in situ hybridization during mouse tooth development.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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