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Study · In vitro (cells) · 2018

2 results

Protective Actions of Anserine Under Diabetic Conditions.

Peters V, Calabrese V, Forsberg E, Volk N, Fleming T, Baelde H, Weigand T, Thiel C, Trovato A, Scuto M, Modafferi S, Schmitt CP

International journal of molecular sciences13 Sept 2018Cited by 57 (Europe PMC)

PMID30217069 (opens in a new tab)PMCIDPMC6164239 (opens in a new tab)DOI10.3390/ijms19092751 (opens in a new tab)PepBankPUB-30217069
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Summary

Background/aimsIn rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine.MethodsAntioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting. In wildtype (WT) and diabetic mice (db/db), the effect of short-term anserine treatment on blood glucose, proteinuria and vascular permeability was measured.ResultsAnserine has a higher antioxidant capacity compared to carnosine (p < 0.001). In tubular cells (HK-2) stressed with 25 mM glucose or 20⁻100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels. In HK-2 cells stressed with glucose, co-incubation with anserine also increased hemeoxygenase (HO-1) protein and reduced total protein carbonylation, but had no effect on cellular sirtuin-1 and thioredoxin protein concentrations. Three intravenous anserine injections every 48 h in 12-week-old db/db mice, improved blood glucose by one fifth, vascular permeability by one third, and halved proteinuria (all p < 0.05).ConclusionAnserine is a potent antioxidant and activates the intracellular Hsp70/HO-1 defense system under oxidative and glycative stress. Short-term anserine treatment in diabetic mice improves glucose homeostasis and nephropathy.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In stressed HK-2 cells, carnosine did not increase Hsp70 mRNA, whereas anserine did.

    In plain wordsIn cells under glucose or peroxide stress, carnosine did not increase Hsp70 mRNA, whereas anserine did.

    Peptide
    L-carnosine
    Outcome
    No significant changeHsp70 mRNA
    Study

    humann: not reported in source

    HK-2 renal tubular cells under glucose or hydrogen peroxide stress
    Outcome group
    Receptor activity and mechanism · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In tubular cells (HK-2) stressed with 25 mM glucose or 20⁻100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels.
    1 context sentence
    Summary · context
    Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting.
  2. 02LabIn vitro (cells)Mechanism

    In stressed HK-2 cells, carnosine did not increase Hsp70 protein, whereas anserine did.

    In plain wordsIn cells under glucose or peroxide stress, carnosine did not increase Hsp70 protein, whereas anserine did.

    Peptide
    L-carnosine
    Outcome
    No significant changeHsp70 protein
    Study

    humann: not reported in source

    HK-2 renal tubular cells under glucose or hydrogen peroxide stress
    Outcome group
    Receptor activity and mechanism · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In tubular cells (HK-2) stressed with 25 mM glucose or 20⁻100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels.
    1 context sentence
    Summary · context
    Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    27 Oct 2026, 18:20 UTC

    Study results updated

  2. Version 1

    27 Oct 2026, 16:32 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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