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Study · Not a test of the peptide alone · 2018

7 results

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils.

Yordanova Y, Vanderlinden W, Stoll R, Rüdiger D, Tosstorff A, Zaremba W, Winter G, Zahler S, Friess W

Scientific reports26 Jul 2018Cited by 3 (Europe PMC)

PMID30050082 (opens in a new tab)PMCIDPMC6062538 (opens in a new tab)DOI10.1038/s41598-018-29529-w (opens in a new tab)PepBankPUB-30050082
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Summary

A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+ in vitro. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) revealed the existence of higher order assembly of Zn2+: GnRH [6-D-Phe]. Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+ and GnRH [6-D-Phe]. Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils. Molecular Dynamic (MD) simulation of the 10:1 Zn2+: GnRH [6-D-Phe] explored the interaction and dimerization processes. In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift. The lyophilized Zn2+: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices. The in vitro release was slow and continuous over 14 days and not influenced by the oil matrix.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Gonadorelin (GnRH, LHRH).

  1. 01LabNot a test of Gonadorelin (GnRH, LHRH) aloneIn vitro (cell-free)Mechanism

    In vitro, the GnRH [6-D-Phe] derivative of gonadorelin self-assembled into nanostructures and fibrils after a pH shift in the presence of Zn2+.

    In plain wordsIn lab tests, a gonadorelin derivative formed nanostructures and fibrils when zinc was present and the pH changed.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableZn2+-triggered self-assembly
    Study

    n: not reported in source

    In vitro peptide assembly with Zn2+
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+ in vitro.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  2. 02LabNot a test of Gonadorelin (GnRH, LHRH) aloneIn vitro (cell-free)Mechanism

    ATR-FTIR spectroscopy showed higher order assembly of Zn2+ with the GnRH [6-D-Phe] derivative.

    In plain wordsIn lab tests, infrared spectroscopy showed that zinc and a gonadorelin derivative formed larger assemblies.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableHigher order assembly by ATR-FTIR
    Study

    n: not reported in source

    In vitro peptide assembly with Zn2+
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) revealed the existence of higher order assembly of Zn2+: GnRH [6-D-Phe].

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  3. 03LabNot a test of Gonadorelin (GnRH, LHRH) aloneIn vitro (cell-free)Mechanism

    NMR spectroscopy indicated a weak interaction between Zn2+ and the GnRH [6-D-Phe] derivative.

    In plain wordsIn lab tests, NMR showed only a weak interaction between zinc and a gonadorelin derivative.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableZn2+ interaction by NMR
    Study

    n: not reported in source

    In vitro peptide assembly with Zn2+
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+ and GnRH [6-D-Phe].

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  4. 04LabNot a test of Gonadorelin (GnRH, LHRH) aloneIn vitro (cell-free)Mechanism

    Atomic force microscopy showed oligomers and fibrils of the GnRH [6-D-Phe] derivative.

    In plain wordsIn lab tests, microscopy showed small clusters and fibrils of a gonadorelin derivative.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableOligomers and fibrils by AFM
    Study

    n: not reported in source

    In vitro peptide assembly with Zn2+
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  5. 05LabNot a test of Gonadorelin (GnRH, LHRH) aloneIn vitro (cell-free)Mechanism

    The GnRH [6-D-Phe] derivative formed nanostructures and fibrils in Tris buffer in a controlled manner through a temperature reduction and a pH shift.

    In plain wordsIn lab tests, a gonadorelin derivative formed fibrils in a controlled way when temperature dropped and pH shifted.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableControlled fibril formation in Tris buffer
    Study

    n: not reported in source

    In vitro peptide assembly with Zn2+
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  6. 06LabNot a test of Gonadorelin (GnRH, LHRH) aloneIn vitro (cell-free)Other

    In vitro, release of GnRH [6-D-Phe] from the Zn2+ assembly in oil vehicle matrices was slow and continuous over 14 days and did not depend on the oil matrix.

    In plain wordsIn lab tests, a gonadorelin derivative was released slowly and steadily over 14 days from oil mixtures, whichever oil was used.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableIn vitro release from oil vehicles
    Study

    n: not reported in source

    In vitro peptide assembly with Zn2+
    Outcome group
    Pharmacokinetics · Pharmacology

    Measurements

    Release duration
    14 days
    in vitro, two oil vehicle matrices

    Source sentences

    Summary
    The in vitro release was slow and continuous over 14 days and not influenced by the oil matrix.
    1 context sentence
    Summary · context
    The lyophilized Zn2+: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  7. 07Computer modelNot a test of Gonadorelin (GnRH, LHRH) aloneIn silicoMechanism

    A molecular dynamics simulation of Zn2+ with the GnRH [6-D-Phe] derivative at 10:1 explored the interaction and dimerization processes.

    In plain wordsA computer simulation explored how zinc and a gonadorelin derivative interact and pair up.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableZn2+ interaction and dimerization by molecular dynamics
    Study

    n: not reported in source

    Molecular dynamics simulation
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Molecular Dynamic (MD) simulation of the 10:1 Zn2+: GnRH [6-D-Phe] explored the interaction and dimerization processes.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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