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Study · Animal study (in vivo) · mouse · 2018

3 results

No effect of thymosin beta-4 on the expression of the transcription factor Islet-1 in the adult murine heart.

Weinberger F, Nicol P, Starbatty J, Stubbendorff M, Becher PM, Schrepfer S, Eschenhagen T

Pharmacology research & perspectives1 Jun 2018Cited by 2 (Europe PMC)

PMID29864245 (opens in a new tab)PMCIDPMC5986028 (opens in a new tab)DOI10.1002/prp2.407 (opens in a new tab)PepBankPUB-29864245
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Summary

The transcription factor Islet-1 marks a progenitor cell population of the second heart field during cardiogenesis. In the adult heart Islet-1 expression is limited to the sinoatrial node, the ventricular outflow tract, and parasympathetic ganglia. The regenerative effect in the injured mouse ventricle of thymosin beta-4 (TB4), a 43-aminoacid peptide, was associated with increased Islet-1 immunostaining, suggesting the induction of an Islet-1-positive progenitor state by TB4. Here we aimed to reassess this effect in a genetic model. Mice from the reporter mouse line Isl1-nLacZ were primed with TB4 and subsequently underwent myocardial infarction. Islet-1 expression was assessed 2, 7, and 14 days after infarction. We detected only a single Islet-1+ cell in 8 TB4 treated and infarcted hearts which located outside of the sinoatrial node, the outflow tract or cardiac ganglia (in ~2500 sections). Two cells were identified in 5 control infarcted hearts. TB4 did not induce LacZ positivity in ventricular explants cultures of Isl1-nLacZ mice nor did it affect the density of LacZ+ cells in explant cultures of nLacZ+ regions of the heart. In summary, we found no evidence that TB4 reactivates Islet-1 expression in adult mouse ventricle.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In Isl1-nLacZ mice primed with TB4 before myocardial infarction, TB4-treated infarcted hearts showed no more Islet-1-positive cells than control infarcted hearts.

    In plain wordsIn mice given thymosin beta‑4 before a heart attack, the hearts showed no more Islet-1 cells than control hearts.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeIslet-1 positive cells in infarcted adult heart
    Study

    mousen = 13

    Isl1-nLacZ reporter mice after myocardial infarction
    Comparator
    Control infarcted hearts
    Outcome group
    Heart repair and regeneration · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We detected only a single Islet-1+ cell in 8 TB4 treated and infarcted hearts which located outside of the sinoatrial node, the outflow tract or cardiac ganglia (in ~2500 sections). Two cells were identified in 5 control infarcted hearts.
    1 context sentence
    Summary · context
    Mice from the reporter mouse line Isl1-nLacZ were primed with TB4 and subsequently underwent myocardial infarction.
  2. 02LabEx vivo tissueMechanism

    In ventricular explant cultures of Isl1-nLacZ mice, TB4 did not induce LacZ positivity.

    In plain wordsIn lab tests on mouse heart tissue, thymosin beta‑4 did not switch on the Islet-1 marker.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeLacZ positivity induced in ventricular explants
    Study

    mousen: not reported in source

    Ventricular explant cultures of Isl1-nLacZ mice
    Outcome group
    Heart repair and regeneration · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    TB4 did not induce LacZ positivity in ventricular explants cultures of Isl1-nLacZ mice
  3. 03LabEx vivo tissueMechanism

    In explant cultures of nLacZ-positive heart regions, TB4 did not change the density of LacZ-positive cells.

    In plain wordsIn lab tests on mouse heart tissue, thymosin beta‑4 did not change the number of Islet-1 marker cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeDensity of LacZ-positive cells in explant cultures
    Study

    mousen: not reported in source

    Explant cultures of nLacZ-positive regions of the heart
    Outcome group
    Heart repair and regeneration · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    nor did it affect the density of LacZ+ cells in explant cultures of nLacZ+ regions of the heart.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

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