Humanin (HN) and glucose transporter 8 (GLUT8) in pregnancies complicated by intrauterine growth restriction.

Janzen C, Lei MYY, Jeong ISD, Ganguly A, Sullivan P, Paharkova V, Capodanno G, Nakamura H, Perry A, Shin BC, Lee KW, Devaskar SU

PloS one28 Mar 2018Cited by 17 (Europe PMC)

PMID29590129PMCIDPMC5873989DOI10.1371/journal.pone.0193583PepBankPUB-29590129

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Summary

BackgroundIntrauterine growth restriction (IUGR) results from a lack of nutrients transferred to the developing fetus, particularly oxygen and glucose. Increased expression of the cytoprotective mitochondrial peptide, humanin (HN), and the glucose transporter 8, GLUT8, has been reported under conditions of hypoxic stress. However, the presence and cellular localization of HN and GLUT8 in IUGR-related placental pathology remain unexplored. Thus, we undertook this study to investigate placental expression of HN and GLUT8 in IUGR-affected versus normal pregnancies.ResultsWe found 1) increased HN expression in human IUGR-affected pregnancies on the maternal aspect of the placenta (extravillous trophoblastic (EVT) cytoplasm) compared to control (i.e. appropriate for gestational age) pregnancies, and a concomitant increase in GLUT8 expression in the same compartment, 2) HN and GLUT8 showed a protein-protein interaction by co-immunoprecipitation, 3) elevated HN and GLUT8 levels in vitro under simulated hypoxia in human EVT cells, HTR8/SVneo, and 4) increased HN expression but attenuated GLUT8 expression in vitro under serum deprivation in HTR8/SVneo cells.ConclusionsThere was elevated HN expression with cytoplasmic localization to EVTs on the maternal aspect of the human placenta affected by IUGR, also associated with increased GLUT8 expression. We found that while hypoxia increased both HN and GLUT8, serum deprivation increased HN expression alone. Also, a protein-protein interaction between HN and GLUT8 suggests that their interaction may fulfill a biologic role that requires interdependency. Future investigations delineating molecular interactions between these proteins are required to fully uncover their role in IUGR-affected pregnancies.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01HumanEndogenous levelObservational studyOther
In human pregnancies with intrauterine growth restriction, the body's own HN expression was increased in extravillous trophoblast cytoplasm on the maternal side of the placenta compared with control pregnancies.
In plain wordsIn pregnancies with restricted fetal growth, the body's own humanin was higher in placenta cells than in normal pregnancies.
Peptide
Humanin
Outcome
IncreaseHN expression in IUGR placenta
Study
n: not reported in source
Comparator
Appropriate for gestational age control pregnancies
Outcome group
Aging markers · Reproductive and sexual health
Source sentences
Summary
increased HN expression in human IUGR-affected pregnancies on the maternal aspect of the placenta (extravillous trophoblastic (EVT) cytoplasm) compared to control (i.e. appropriate for gestational age) pregnancies
1 context sentence(design, population, supporting numbers)
Summary · context
Thus, we undertook this study to investigate placental expression of HN and GLUT8 in IUGR-affected versus normal pregnancies.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2959012901
02LabEndogenous levelEx vivo tissueOther
HN and GLUT8 showed a protein-protein interaction by co-immunoprecipitation.
In plain wordsIn lab tests, the body's own humanin and the glucose transporter GLUT8 were found to bind each other.
Peptide
Humanin
Outcome
Not applicableHN and GLUT8 protein-protein interaction
Study
humann: not reported in source
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
HN and GLUT8 showed a protein-protein interaction by co-immunoprecipitation
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2959012902
03LabEndogenous levelIn vitro (cells)Other
In human EVT cells (HTR8/SVneo), simulated hypoxia elevated the cells' own HN levels.
In plain wordsIn human placenta cells, low oxygen raised the cells' own humanin levels.
Peptide
Humanin
Outcome
IncreaseHN level under simulated hypoxia
Study
humann: not reported in source
HTR8/SVneo human EVT cells
Outcome group
Aging markers · Reproductive and sexual health
Source sentences
Summary
We found that while hypoxia increased both HN and GLUT8, serum deprivation increased HN expression alone.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2959012903
04LabEndogenous levelIn vitro (cells)Other
In human EVT cells (HTR8/SVneo), serum deprivation increased the cells' own HN expression.
In plain wordsIn human placenta cells, removing serum raised the cells' own humanin expression.
Peptide
Humanin
Outcome
IncreaseHN expression under serum deprivation
Study
humann: not reported in source
HTR8/SVneo human EVT cells
Outcome group
Aging markers · Reproductive and sexual health
Source sentences
Summary
We found that while hypoxia increased both HN and GLUT8, serum deprivation increased HN expression alone.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2959012904

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Version history

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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