Effects of Huang Bai (Phellodendri Cortex) on bone growth and pubertal development in adolescent female rats.

Lee SH, Lee HJ, Lee SH, Kim YS, Lee D, Chun J, Lee JY, Kim H, Chang GT

Chinese medicine12 Jan 2018Cited by 6 (Europe PMC)

PMID29344080PMCIDPMC5767045DOI10.1186/s13020-017-0156-7PepBankPUB-29344080

Summary

BackgroundTo evaluate the effects of Huang Bai (Phellodendron amurense) on growth and maturation in adolescent female rats.MethodsFemale Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg). Body weight, food intake, and vaginal opening were measured daily from postnatal day (PND) 28 to PND 43. Tetracycline (20 mg/kg) was injected on PND 41. After sacrifice on PND 43, the ovaries and uterus were weighed, and the tibias were fixed in 4% paraformaldehyde. Decalcified and dehydrated tibias were sectioned at a thickness of 40 μm, and sectioned tissues were examined with a fluorescence microscope. Insulin-like growth factor (IGF)-1 and bone morphogenetic protein (BMP)-2 were detected using immunohistochemistry.ResultsRelative to controls, body weight was higher in the triptorelin group. Bone growth rate increased in the Huang Bai 100 mg/kg (354.00 ± 31.1 μm/day), rhGH (367.10 ± 27.11 μm/day), and triptorelin (374.50 ± 25.37 μm/day) groups. Expression of IGF-1 and BMP-2 in the hypertrophic zone was higher in all experimental groups. Vaginal opening occurred earlier in the estradiol group (PND 33.58 ± 1.62) than in controls and later in the triptorelin group (PND > 43). Ovarian and uterine weights were lower in the oestradiol and triptorelin groups. However, Huang Bai had nonsignificant effects on vaginal opening and the weights of ovaries and the uterus.ConclusionsHuang Bai stimulated bone growth by upregulating IGF-1 and BMP-2 in the growth plate. However, it had no effect on pubertal development.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In adolescent female rats, triptorelin increased body weight versus controls.
In plain wordsIn rats, triptorelin increased body weight compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseBody weight
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Body weight · Metabolic and weight
Studied dose
100 μgAs given in this study (rat).
Source sentences
Summary
Relative to controls, body weight was higher in the triptorelin group.
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408001
02AnimalAnimal study (in vivo)Efficacy outcome
In adolescent female rats, triptorelin increased bone growth rate.
In plain wordsIn rats, triptorelin increased bone growth rate compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseBone growth rate
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Bone and joint · Musculoskeletal
Studied dose
100 μgAs given in this study (rat).
Measurements
Bone growth rate
374.5 μm/day
triptorelin
Source sentences
Summary
Bone growth rate increased in the Huang Bai 100 mg/kg (354.00 ± 31.1 μm/day), rhGH (367.10 ± 27.11 μm/day), and triptorelin (374.50 ± 25.37 μm/day) groups.
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408002
03AnimalAnimal study (in vivo)Mechanism
In adolescent female rats, triptorelin increased hypertrophic-zone IGF-1 expression versus controls.
In plain wordsIn rats, triptorelin increased growth plate IGF-1 expression compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseIGF-1 expression
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Growth hormone and IGF-1 · Musculoskeletal
Studied dose
100 μgAs given in this study (rat).
Source sentences
Summary
Expression of IGF-1 and BMP-2 in the hypertrophic zone was higher in all experimental groups.
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408003
04AnimalAnimal study (in vivo)Mechanism
In adolescent female rats, triptorelin increased hypertrophic-zone BMP-2 expression versus controls.
In plain wordsIn rats, triptorelin increased growth plate BMP-2 expression compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseBMP-2 expression
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Bone and joint · Musculoskeletal
Studied dose
100 μgAs given in this study (rat).
Source sentences
Summary
Expression of IGF-1 and BMP-2 in the hypertrophic zone was higher in all experimental groups.
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408004
05AnimalAnimal study (in vivo)Efficacy outcome
In adolescent female rats, triptorelin delayed vaginal opening versus controls.
In plain wordsIn rats, triptorelin delayed vaginal opening compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseAge at vaginal opening
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100 μgAs given in this study (rat).
Measurements
Vaginal opening
> 43 PND
triptorelin
Source sentences
Summary
Vaginal opening occurred earlier in the estradiol group (PND 33.58 ± 1.62) than in controls and later in the triptorelin group (PND > 43).
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408005
06AnimalAnimal study (in vivo)Efficacy outcome
In adolescent female rats, triptorelin reduced ovarian weight.
In plain wordsIn rats, triptorelin reduced ovarian weight compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreaseOvarian weight
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100 μgAs given in this study (rat).
Source sentences
Summary
Ovarian and uterine weights were lower in the oestradiol and triptorelin groups.
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408006
07AnimalAnimal study (in vivo)Efficacy outcome
In adolescent female rats, triptorelin reduced uterine weight.
In plain wordsIn rats, triptorelin reduced uterine weight compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreaseUterine weight
Study
ratn: not reported in source
Adolescent female Sprague-Dawley rats
Comparator
Distilled water control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100 μgAs given in this study (rat).
Source sentences
Summary
Ovarian and uterine weights were lower in the oestradiol and triptorelin groups.
1 context sentence(design, population, supporting numbers)
Summary · context
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg).
Show in the study text Report a problemFND-2934408007

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

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Version 2Current
22 Oct 2026, 02:26 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

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