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Study · Animal study (in vivo) · zebrafish · 2017

6 results

SS-31 peptide enables mitochondrial targeting drug delivery: a promising therapeutic alteration to prevent hair cell damage from aminoglycosides.

Kuang X, Zhou S, Guo W, Wang Z, Sun Y, Liu H

Drug delivery1 Nov 2017Cited by 38 (Europe PMC)

PMID29214897 (opens in a new tab)PMCIDPMC8241023 (opens in a new tab)DOI10.1080/10717544.2017.1402220 (opens in a new tab)PepBankPUB-29214897
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Summary

Aminoglycoside-induced hearing loss stems from damage or loss of mechanosensory hair cells in the inner ear. Intrinsic mitochondrial cell death pathway plays a key role in that cellular dysfunction for which no proven effective therapies against oto-toxicities exist. Therefore, the aim of the present study was to develop a new mitochondrial targeting drug delivery system (DDS) that provided improved protection from gentamicin. Particularly, SS-31 peptide-conjugated geranylgeranylacetone (GGA) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles were constructed successfully via emulsion-solvent evaporation method. The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin. SS-31 modification significantly reduced the activity of mechanoelectrical transduction (MET) channel and gentamicin uptake in zebrafish lateral line hair cells. As expected, SS-31 conjugated nanoparticles showed mitochondrial specific accumulation in hair cells when compared with unconjugated formulations. Furthermore, intracellular SS-31 modified PLGA NPs slightly enhanced mitochondrial membrane potential (MMP, ΔΨm) and then returned to a steady-state, indicating their effect on the respiratory chain complexes in mitochondria. GGA loaded SS-31 conjugated nanoparticles demonstrated the most favorable hair cells survivals against gentamicin when compared with unconjugated groups whereas blank formulations failed to exhibit potency, indicating that the efficiency was attributed to drug delivery of GGA. These results suggest that our constructed mitochondria-targeting PLGA based DDS have potential application in protecting hair cells from ototoxic agents.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In zebrafish lateral line hair cells, SS‑31 modification significantly reduced the activity of the mechanoelectrical transduction channel.

    In plain wordsIn zebrafish, SS‑31 modification lowered the activity of the MET channel in hair cells.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseMechanoelectrical transduction channel activity
    Study

    zebrafishn: not reported in source

    Zebrafish lateral line hair cells exposed to gentamicin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    SS-31 modification significantly reduced the activity of mechanoelectrical transduction (MET) channel
    1 context sentence
    Summary · context
    The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In zebrafish lateral line hair cells, SS‑31 modification significantly reduced gentamicin uptake.

    In plain wordsIn zebrafish, SS‑31 modification lowered gentamicin uptake into hair cells.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseGentamicin uptake
    Study

    zebrafishn: not reported in source

    Zebrafish lateral line hair cells exposed to gentamicin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    SS-31 modification significantly reduced the activity of mechanoelectrical transduction (MET) channel and gentamicin uptake in zebrafish lateral line hair cells.
    1 context sentence
    Summary · context
    The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin.

Studies that did not test the peptide alone4

In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Elamipretide (SS-31).

  1. 03AnimalNot a test of Elamipretide (SS-31) aloneAnimal study (in vivo)Mechanism

    In zebrafish hair cells, SS‑31 conjugated nanoparticles showed mitochondrial specific accumulation compared with unconjugated formulations.

    In plain wordsIn zebrafish, nanoparticles carrying SS‑31 gathered in hair-cell mitochondria more than versions without SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseMitochondrial accumulation in hair cells
    Study

    zebrafishn: not reported in source

    Zebrafish lateral line hair cells exposed to gentamicin
    Comparator
    Unconjugated formulations
    Outcome group
    Pharmacokinetics · Nervous system and cognition

    Source sentences

    Summary
    As expected, SS-31 conjugated nanoparticles showed mitochondrial specific accumulation in hair cells when compared with unconjugated formulations.
    1 context sentence
    Summary · context
    The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin.

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

  2. 04AnimalNot a test of Elamipretide (SS-31) aloneAnimal study (in vivo)Mechanism

    In zebrafish hair cells, intracellular SS‑31 modified PLGA nanoparticles slightly enhanced mitochondrial membrane potential and then it returned to a steady state.

    In plain wordsIn zebrafish, SS‑31 modified nanoparticles slightly raised mitochondrial membrane potential in hair cells, which then returned to a steady state.

    Peptide
    Elamipretide (SS-31)
    Outcome
    MixedMitochondrial membrane potential
    Study

    zebrafishn: not reported in source

    Zebrafish lateral line hair cells exposed to gentamicin
    Outcome group
    Mitochondrial function · Nervous system and cognition

    Source sentences

    Summary
    Furthermore, intracellular SS-31 modified PLGA NPs slightly enhanced mitochondrial membrane potential (MMP, ΔΨm) and then returned to a steady-state, indicating their effect on the respiratory chain complexes in mitochondria.
    1 context sentence
    Summary · context
    The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin.

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

  3. 05AnimalNot a test of Elamipretide (SS-31) aloneAnimal study (in vivo)Efficacy outcome

    In zebrafish, GGA loaded SS‑31 conjugated nanoparticles gave the most favourable hair cell survival against gentamicin compared with unconjugated groups.

    In plain wordsIn zebrafish, GGA-loaded nanoparticles carrying SS‑31 protected hair cells from gentamicin better than versions without SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseHair cell survival against gentamicin
    Study

    zebrafishn: not reported in source

    Zebrafish lateral line hair cells exposed to gentamicin
    Comparator
    Unconjugated groups
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    GGA loaded SS-31 conjugated nanoparticles demonstrated the most favorable hair cells survivals against gentamicin when compared with unconjugated groups
    1 context sentence
    Summary · context
    The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin.

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

  4. 06AnimalNot a test of Elamipretide (SS-31) aloneAnimal study (in vivo)Efficacy outcome

    In zebrafish, blank nanoparticle formulations failed to show potency against gentamicin damage to hair cells.

    In plain wordsIn zebrafish, blank nanoparticles without the loaded drug did not protect hair cells from gentamicin.

    Peptide
    Elamipretide (SS-31)
    Outcome
    No significant changeHair cell survival with blank formulations
    Study

    zebrafishn: not reported in source

    Zebrafish lateral line hair cells exposed to gentamicin
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    whereas blank formulations failed to exhibit potency, indicating that the efficiency was attributed to drug delivery of GGA.
    1 context sentence
    Summary · context
    The zebrafish lateral line sensory system was used as an in vivo evaluating platform to investigate the protective efficiency against gentamicin.

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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