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Study · In vitro (cells) · bovine · 2017

10 results

Kisspeptin-10 Induces β-Casein Synthesis via GPR54 and Its Downstream Signaling Pathways in Bovine Mammary Epithelial Cells.

Sun J, Liu J, Huang B, Kan X, Chen G, Wang W, Fu S

International journal of molecular sciences5 Dec 2017Cited by 14 (Europe PMC)

PMID29206176 (opens in a new tab)PMCIDPMC5751224 (opens in a new tab)DOI10.3390/ijms18122621 (opens in a new tab)PepBankPUB-29206176
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Summary

Kisspeptins (Kps) play a key role in the regulation of GnRH axis and as an anti-metastasis agent by binding with GPR54. Recently, we observed that the expression of GPR54 was higher in the lactating mammary tissues of dairy cows with high-quality milk (0.81 ± 0.13 kg/day of milk protein yield; 1.07 ± 0.18 kg/day of milk fat yield) than in those with low-quality milk (0.51 ± 0.14 kg/day of milk protein yield; 0.67 ± 0.22 kg/day of milk fat yield). We hypothesized that Kp-10 might regulate the milk protein, β-casein (CSN2) synthesis via GPR54 and its downstream signaling. First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10. Compared with the control cells, the synthesis of CSN2 is significantly increased at a concentration of 100 nM of Kp-10. In addition, the increased effect of CSN2 synthesis was blocked when the cells were pre-treated with the selective inhibitor of GPR54 Peptide-234 (P-234). Mechanistic study revealed that Kp-10 activated ERK1/2, AKT, mTOR and STAT5 in bMECs. Moreover, inhibiting ERK1/2, AKT, mTOR and STAT5 with U0126, MK2206, Rapamycin and AG490 could block the effects of Kp-10. Together, these results demonstrate that Kp-10 facilitates the synthesis of CSN2 via GPR54 and its downstream signaling pathways mTOR, ERK1/2, STAT5 and AKT.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In bovine mammary epithelial cells, Kp-10 at 100 nM significantly increased β-casein synthesis compared with control cells.

    In plain wordsIn lab-grown cow milk-gland cells, Kp-10 increased production of a milk protein compared with untreated cells.

    Peptide
    Kisspeptin-10
    Outcome
    Increaseβ-casein (CSN2) synthesis
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Comparator
    Control cells
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    100 nMAs given in this study (bovine).

    Measurements

    concentration
    100 nM
    Kp-10

    Source sentences

    Summary
    Compared with the control cells, the synthesis of CSN2 is significantly increased at a concentration of 100 nM of Kp-10.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  2. 02LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, the Kp-10-induced increase in CSN2 synthesis was blocked by pre-treatment with the GPR54 inhibitor Peptide-234.

    In plain wordsIn lab-grown cow milk-gland cells, blocking the GPR54 receptor stopped the Kp-10 rise in milk protein.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeCSN2 synthesis with GPR54 inhibitor P-234
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Comparator
    Kp-10 without P-234
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, the increased effect of CSN2 synthesis was blocked when the cells were pre-treated with the selective inhibitor of GPR54 Peptide-234 (P-234).
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  3. 03LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, Kp-10 activated ERK1/2.

    In plain wordsIn lab-grown cow milk-gland cells, Kp-10 switched on the ERK1/2 signal.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseERK1/2 activation
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Mechanistic study revealed that Kp-10 activated ERK1/2, AKT, mTOR and STAT5 in bMECs.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  4. 04LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, Kp-10 activated AKT.

    In plain wordsIn lab-grown cow milk-gland cells, Kp-10 switched on the AKT signal.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseAKT activation
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Mechanistic study revealed that Kp-10 activated ERK1/2, AKT, mTOR and STAT5 in bMECs.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  5. 05LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, Kp-10 activated mTOR.

    In plain wordsIn lab-grown cow milk-gland cells, Kp-10 switched on the mTOR signal.

    Peptide
    Kisspeptin-10
    Outcome
    IncreasemTOR activation
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Mechanistic study revealed that Kp-10 activated ERK1/2, AKT, mTOR and STAT5 in bMECs.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  6. 06LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, Kp-10 activated STAT5.

    In plain wordsIn lab-grown cow milk-gland cells, Kp-10 switched on the STAT5 signal.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseSTAT5 activation
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Mechanistic study revealed that Kp-10 activated ERK1/2, AKT, mTOR and STAT5 in bMECs.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  7. 07LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, inhibiting ERK1/2 with U0126 could block the effects of Kp-10.

    In plain wordsIn lab-grown cow milk-gland cells, blocking ERK1/2 stopped the Kp-10 effects.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKp-10 effect after ERK1/2 inhibition (U0126)
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Comparator
    Kp-10 without inhibitor
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, inhibiting ERK1/2, AKT, mTOR and STAT5 with U0126, MK2206, Rapamycin and AG490 could block the effects of Kp-10.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  8. 08LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, inhibiting AKT with MK2206 could block the effects of Kp-10.

    In plain wordsIn lab-grown cow milk-gland cells, blocking AKT stopped the Kp-10 effects.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKp-10 effect after AKT inhibition (MK2206)
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Comparator
    Kp-10 without inhibitor
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, inhibiting ERK1/2, AKT, mTOR and STAT5 with U0126, MK2206, Rapamycin and AG490 could block the effects of Kp-10.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  9. 09LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, inhibiting mTOR with rapamycin could block the effects of Kp-10.

    In plain wordsIn lab-grown cow milk-gland cells, blocking mTOR stopped the Kp-10 effects.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKp-10 effect after mTOR inhibition (rapamycin)
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Comparator
    Kp-10 without inhibitor
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, inhibiting ERK1/2, AKT, mTOR and STAT5 with U0126, MK2206, Rapamycin and AG490 could block the effects of Kp-10.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.
  10. 10LabIn vitro (cells)Mechanism

    In bovine mammary epithelial cells, inhibiting STAT5 with AG490 could block the effects of Kp-10.

    In plain wordsIn lab-grown cow milk-gland cells, blocking STAT5 stopped the Kp-10 effects.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKp-10 effect after STAT5 inhibition (AG490)
    Study

    bovinen: not reported in source

    Bovine mammary epithelial cells (bMECs)
    Comparator
    Kp-10 without inhibitor
    Outcome group
    Lactation and milk synthesis · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, inhibiting ERK1/2, AKT, mTOR and STAT5 with U0126, MK2206, Rapamycin and AG490 could block the effects of Kp-10.
    1 context sentence
    Summary · context
    First, we isolated the bovine mammary epithelial cells (bMECs) from lactating Holstein dairy cows, and treated them with different concentrations of Kp-10.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

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Terms as the source lists them.

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