Humanin analogue, S14G-humanin, has neuroprotective effects against oxygen glucose deprivation/reoxygenation by reactivating Jak2/Stat3 signaling through the PI3K/AKT pathway.

Gao GS, Li Y, Zhai H, Bi JW, Zhang FS, Zhang XY, Fan SH

Experimental and therapeutic medicine16 Aug 2017Cited by 27 (Europe PMC)

PMID29043002PMCIDPMC5639330DOI10.3892/etm.2017.4934PepBankPUB-29043002

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In SH-SY5Y cells exposed to OGD/R, HNG blocked the OGD/R-induced reduction of cell viability, with the most evident effect at 1 µg/l.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, HNG blocked the drop in cell survival.
Peptide
Humanin
Outcome
IncreaseCell viability after OGD/R
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
0-10 µg/lAs given in this study (human).
Source sentences
Summary
Adding HNG (0-10 µg/l) to SH-SY5Y cells to different extents blocked OGD/R-induced reduction of cell viability
2 context sentences(design, population, supporting numbers)
Summary · context
with the most evident effects at 1 µg/l HNG.
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
Report a problemFND-2904300201
02LabIn vitro (cells)Efficacy outcome
In SH-SY5Y cells exposed to OGD/R, HNG blocked the OGD/R-induced reduction of antioxidative capacity.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, HNG blocked the drop in antioxidant capacity.
Peptide
Humanin
Outcome
IncreaseAntioxidative capacity after OGD/R
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
0-10 µg/lAs given in this study (human).
Source sentences
Summary
Adding HNG (0-10 µg/l) to SH-SY5Y cells to different extents blocked OGD/R-induced reduction of cell viability and antioxidative capacity, as well as decreased the elevated apoptosis rate induced by OGD/R, with the most evident effects at 1 µg/l HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
Report a problemFND-2904300202
03LabIn vitro (cells)Efficacy outcome
In SH-SY5Y cells exposed to OGD/R, HNG decreased the elevated apoptosis rate induced by OGD/R.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, HNG lowered the raised rate of cell death.
Peptide
Humanin
Outcome
DecreaseApoptosis rate after OGD/R
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
0-10 µg/lAs given in this study (human).
Source sentences
Summary
Adding HNG (0-10 µg/l) to SH-SY5Y cells to different extents blocked OGD/R-induced reduction of cell viability and antioxidative capacity, as well as decreased the elevated apoptosis rate induced by OGD/R, with the most evident effects at 1 µg/l HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
Report a problemFND-2904300203
04LabIn vitro (cells)Mechanism
In SH-SY5Y cells exposed to OGD/R, HNG treatment reactivated Jak2/Stat3 signaling that OGD/R had attenuated.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, HNG switched the Jak2/Stat3 signal back on.
Peptide
Humanin
Outcome
IncreaseJak2/Stat3 signaling
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Janus kinase 2 (Jak2)/signal transducer and activator of transcription 3 (Stat3) signaling was attenuated in OGD/R processes, yet reactivated with HNG treatment.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
Report a problemFND-2904300204

Studies that did not test the peptide alone3

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

05LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In SH-SY5Y cells exposed to OGD/R, the Jak2/Stat3 inhibitor FLLL32 abolished the anti-apoptotic and antioxidant effects of HNG.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, blocking the Jak2/Stat3 signal removed the protective effects of HNG.
Peptide
Humanin
Outcome
Not applicableHNG effects on anti-apoptosis and antioxidation with Jak2/Stat3 inhibitor FLLL32
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
FLLL32 (5 µM), a specific inhibitor of the signal, abolished effects of HNG on anti-apoptosis and antioxidation in OGD/R processes.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-2904300205
06LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In SH-SY5Y cells exposed to OGD/R, co-treatment with HNG and FLLL32 failed to interrupt the upregulation of cytochrome c, Bax and cleaved caspase-3.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, HNG plus the inhibitor FLLL32 did not stop the rise in cell-death proteins.
Peptide
Humanin
Outcome
No significant changeCytochrome c, Bax and cleaved caspase-3 upregulation with HNG plus FLLL32
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Co-treatment with HNG and FLLL32 failed to interrupt upregulation of cytochrome c, B-cell lymphoma 2-associated X protein and cleaved caspase-3 provoked by OGD/R.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-2904300206
07LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In SH-SY5Y cells exposed to OGD/R, the PI3K inhibitor LY294002 or the AKT inhibitor MK-2206 repressed the Jak2/Stat3 signaling that HNG had activated.
In plain wordsIn lab-grown nerve cells under oxygen and glucose stress, blocking PI3K or AKT reduced the Jak2/Stat3 signal that HNG had switched on.
Peptide
Humanin
Outcome
DecreaseJak2/Stat3 signaling activated by HNG with PI3K or AKT inhibitors
Study
humann: not reported in source
SH-SY5Y neuroblastoma cells with oxygen glucose deprivation/reoxygenation (OGD/R)
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Similar to FLLL32, Jak2/Stat3 signaling activated by HNG was also repressed by inhibitor of phosphoinositide 3-kinase (PI3K; 10 µM LY294002) or protein kinase B (AKT; 5 µM MK-2206 2HCl).
1 context sentence(design, population, supporting numbers)
Summary · context
The present study established an in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y neuroblastoma cells to mimic the in vivo ischemia/reperfusion injury in stroke.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-2904300207

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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