The effects of kisspeptin-10 on serum metabolism and myocardium in rats.

Zhang Y, Hou Y, Wang X, Ping J, Ma Z, Suo C, Lei Z, Li X, Zhang Z, Jia C, Su J

PloS one10 Jul 2017Cited by 10 (Europe PMC)

PMID28692647PMCIDPMC5503227DOI10.1371/journal.pone.0179164PepBankPUB-28692647

Summary

Kisspeptin is a peptide encoded by the Kiss 1 gene and is also called metastin. Previous studies have generally focused on several functions of this peptide, including metastasis, puberty, vasoconstriction and reproduction. However, few studies have focused on the cardiac functions of kisspeptin. In the present study, cardiac histomorphology was observed via TEM (transmission electron microscope) and HE and Masson staining to observe instinctive changes. Serum metabolites levels were also measured and analyzed using GC/TOF-MS after injection with kisspeptin-10. A gene chip was employed to screen the potential genes and pathways in the myocardium at the transcriptional leve, while RT-PCR and Western Blot were conducted to verify the relevant mRNA and protein expression, respectively. Histopathological findings demonstrated that there were many irregular wavy contractions through HE staining and increased fibrosis around the heart cells through Masson staining after treatment with kisspeptin-10. Additionally, the main changes in ultrastructure, including changes in mitochondrial and broken mitochondrial cristae, could be observed with TEM after treatment with kisspeptin-10. The PCA scores plot of the serum metabolites was in the apparent partition after injection of kisspeptin-10. Twenty-six obviously changed metabolites were detected and classified as amino acids, carbohydrate metabolites, organic acids and other metabolites. Furthermore, gene chip analysis showed 1112 differentially expressed genes after treatment with kisspeptin-10, including 330 up-regulated genes and 782 down-regulated genes. These genes were enriched in several signaling pathways related to heart diseases. The RT-PCR result for ITGB8, ITGA4, ITGB7, MYL7, HIF1-α and BNP corresponded with the gene chip assay. Moreover, the upregulated genes ITGB8, ITGA4 and BNP also displayed consistent protein levels in Western Blot results. In summary, these findings suggest that kisspeptin-10 could alter the morphology and structure of myocardial cells, serum metabolite levels, and expression of genes and proteins in heart tissues. Our work determined the profound effects of kisspeptin-10 on the heart, which could further lead to the development of therapeutics related to kisspeptin-10, including antagonists and analogs.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Other
In rats, kisspeptin‑10 treatment was followed by many irregular wavy contractions of heart cells on HE staining.
In plain wordsIn rats, kisspeptin‑10 treatment was followed by irregular wavy contractions in heart cells.
Peptide
Kisspeptin-10
Outcome
IncreaseMyocardial wavy contractions (HE staining)
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Heart function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Histopathological findings demonstrated that there were many irregular wavy contractions through HE staining
Show in the study text Report a problemFND-2869264701
02AnimalAnimal study (in vivo)Other
In rats, kisspeptin‑10 treatment was followed by increased fibrosis around the heart cells on Masson staining.
In plain wordsIn rats, kisspeptin‑10 treatment was followed by more scar-like tissue around heart cells.
Peptide
Kisspeptin-10
Outcome
IncreaseMyocardial fibrosis (Masson staining)
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Heart function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
increased fibrosis around the heart cells through Masson staining after treatment with kisspeptin-10.
Show in the study text Report a problemFND-2869264702
03AnimalAnimal study (in vivo)Other
In rats, kisspeptin‑10 treatment changed myocardial ultrastructure, including mitochondrial changes and broken mitochondrial cristae, on TEM.
In plain wordsIn rats, kisspeptin‑10 treatment changed the fine structure of heart cells, including damaged mitochondria.
Peptide
Kisspeptin-10
Outcome
MixedMyocardial ultrastructure (mitochondria)
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Heart function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Additionally, the main changes in ultrastructure, including changes in mitochondrial and broken mitochondrial cristae, could be observed with TEM after treatment with kisspeptin-10.
Show in the study text Report a problemFND-2869264703
04AnimalAnimal study (in vivo)Other
In rats, the PCA scores plot of serum metabolites separated apparently after injection of kisspeptin‑10.
In plain wordsIn rats, kisspeptin‑10 injection changed the overall pattern of blood metabolites.
Peptide
Kisspeptin-10
Outcome
MixedSerum metabolite profile (PCA)
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Metabolic pathways and metabolomics · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
The PCA scores plot of the serum metabolites was in the apparent partition after injection of kisspeptin-10.
Show in the study text Report a problemFND-2869264704
05AnimalAnimal study (in vivo)Other
In rats, twenty-six serum metabolites changed obviously after kisspeptin‑10, in classes including amino acids, carbohydrate metabolites and organic acids.
In plain wordsIn rats, kisspeptin‑10 changed twenty-six blood metabolites, including amino acids and sugars.
Peptide
Kisspeptin-10
Outcome
MixedNumber of changed serum metabolites
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Metabolic pathways and metabolomics · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Twenty-six obviously changed metabolites were detected and classified as amino acids, carbohydrate metabolites, organic acids and other metabolites.
Show in the study text Report a problemFND-2869264705
06AnimalAnimal study (in vivo)Other
In rat myocardium, gene chip analysis showed 1112 differentially expressed genes after kisspeptin‑10, 330 up-regulated and 782 down-regulated.
In plain wordsIn rat heart tissue, kisspeptin‑10 changed the activity of 1112 genes: 330 went up and 782 went down.
Peptide
Kisspeptin-10
Outcome
MixedDifferentially expressed myocardial genes
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Gene expression · Cardiovascular
Studied dose
Dose not stated
Measurements
differentially expressed genes
1112 genes
myocardium
up-regulated genes
330 genes
myocardium
down-regulated genes
782 genes
myocardium
Source sentences
Summary
Furthermore, gene chip analysis showed 1112 differentially expressed genes after treatment with kisspeptin-10, including 330 up-regulated genes and 782 down-regulated genes.
Show in the study text Report a problemFND-2869264706
07AnimalAnimal study (in vivo)Mechanism
In rat myocardium, the differentially expressed genes were enriched in several signaling pathways related to heart diseases.
In plain wordsIn rat heart tissue, the genes changed by kisspeptin‑10 belong to pathways linked to heart disease.
Peptide
Kisspeptin-10
Outcome
Not applicableSignaling pathways of differentially expressed genes
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Gene expression · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
These genes were enriched in several signaling pathways related to heart diseases.
Show in the study text Report a problemFND-2869264707
08AnimalAnimal study (in vivo)Mechanism
In rat myocardium, RT-PCR results for ITGB8, ITGA4, ITGB7, MYL7, HIF1-α and BNP corresponded with the gene chip assay.
In plain wordsIn rat heart tissue, a second test confirmed the gene chip changes for six genes after kisspeptin‑10.
Peptide
Kisspeptin-10
Outcome
Not applicableRT-PCR confirmation of selected gene changes
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Gene expression · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
The RT-PCR result for ITGB8, ITGA4, ITGB7, MYL7, HIF1-α and BNP corresponded with the gene chip assay.
Show in the study text Report a problemFND-2869264708
09AnimalAnimal study (in vivo)Mechanism
In rat myocardium, the upregulated genes ITGB8, ITGA4 and BNP also showed consistent protein levels on Western blot.
In plain wordsIn rat heart tissue, higher activity of three genes after kisspeptin‑10 matched higher protein levels.
Peptide
Kisspeptin-10
Outcome
IncreaseITGB8, ITGA4 and BNP protein levels
Study
ratn: not reported in source
Rat myocardium and serum
Outcome group
Gene expression · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Moreover, the upregulated genes ITGB8, ITGA4 and BNP also displayed consistent protein levels in Western Blot results.
Show in the study text Report a problemFND-2869264709

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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