In MALDI imaging of tissue, a supercritical carbon dioxide wash raised the signal-to-noise ratio of the thymosin β4 peak almost 190-fold above control.
In plain wordsIn lab tests of tissue, a washing step made the thymosin β4 signal almost 190 times clearer than control.
- Peptide
- Thymosin beta-4 (human)
- Outcome
- IncreaseSignal-to-noise ratio of the thymosin β4 peak after supercritical fluid wash
- Study
n: not reported in source
tissue sections (MALDI-IMS)- Comparator
- Control
- Outcome group
- Analytical methods and detection · Pharmacology
Measurements
- Signal-to-noise ratio increase
- ≈ 190-fold
- m/z 4963.7 (thymosin β4) vs control
Source sentences
The greatest improvement in the signal-to-noise ratio (S/N) was found at m/z 4963.7, which was identified as thymosin β4, with the S/N reaching almost 190-fold higher than the control.
1 context sentence(design, population, supporting numbers)
To improve the sensitivity of peptide detection, we used supercritical fluid of CO2 (scCO2) as washing solvent for a pretreatment to remove lipids.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).