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Study · In vitro (cells) · mouse · 2016

21 results

Thymosin β4 impeded murine stem cell proliferation with an intact cardiovascular differentiation.

Nie L, Gao SJ, Zhao YN, Masika J, Luo HY, Hu XW, Zhang LP, Zeng Y, Hescheler J, Liang HM

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban1 Jun 2016Cited by 5 (Europe PMC)

PMID27376799 (opens in a new tab)DOI10.1007/s11596-016-1587-6 (opens in a new tab)PepBankPUB-27376799
Publisher pagePubMedAsk about this study
Jump to 21 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study21

21 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In mouse embryonic stem cells, Tβ4 decreased proliferation in a partly dose-dependent manner.

    In plain wordsIn mouse stem cells, Tβ4 slowed cell growth.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreasemESC proliferation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It was found that Tβ4 decreased mESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  2. 02LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 decreased expression of c-myc.

    In plain wordsIn mouse stem cells, Tβ4 lowered activity of a cell-cycle gene, c-myc.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Decreasec-myc expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It was found that Tβ4 decreased mESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  3. 03LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 decreased expression of c-fos.

    In plain wordsIn mouse stem cells, Tβ4 lowered activity of a cell-cycle gene, c-fos.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Decreasec-fos expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It was found that Tβ4 decreased mESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  4. 04LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 decreased expression of c-jun.

    In plain wordsIn mouse stem cells, Tβ4 lowered activity of a cell-cycle gene, c-jun.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Decreasec-jun expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It was found that Tβ4 decreased mESCs proliferation in a partial dose-dependent manner and the expression of cell cycle regulatory genes c-myc, c-fos and c-jun.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  5. 05LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, the self-renewal marker Oct4 was elevated with Tβ4.

    In plain wordsIn mouse stem cells, Tβ4 raised a marker of self-renewal, Oct4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseOct4 self-renewal marker
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, mESCs self-renewal markers Oct4 and Nanog were elevated, indicating the maintenance of self-renewal ability in these mESCs.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  6. 06LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, the self-renewal marker Nanog was elevated with Tβ4.

    In plain wordsIn mouse stem cells, Tβ4 raised a marker of self-renewal, Nanog.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNanog self-renewal marker
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, mESCs self-renewal markers Oct4 and Nanog were elevated, indicating the maintenance of self-renewal ability in these mESCs.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  7. 07LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 inhibited STAT3 phosphorylation.

    In plain wordsIn mouse stem cells, Tβ4 reduced STAT3 signalling.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseSTAT3 phosphorylation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  8. 08LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 inhibited Akt phosphorylation.

    In plain wordsIn mouse stem cells, Tβ4 reduced Akt signalling.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseAkt phosphorylation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  9. 09LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 enhanced RAS expression.

    In plain wordsIn mouse stem cells, Tβ4 raised RAS levels.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseRAS expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  10. 10LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 enhanced ERK phosphorylation.

    In plain wordsIn mouse stem cells, Tβ4 increased ERK signalling.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseERK phosphorylation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Phosphorylation of STAT3 and Akt was inhibited by Tβ4 while the expression of RAS and phosphorylation of ERK were enhanced.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  11. 11LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, no significant difference was found in BMP2 or BMP4 with Tβ4.

    In plain wordsIn mouse stem cells, Tβ4 did not significantly change BMP2 or BMP4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeBMP2/BMP4 expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    No significant difference was found in BMP2/BMP4 or their downstream protein smad.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  12. 12LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, no significant difference was found in the downstream protein smad with Tβ4.

    In plain wordsIn mouse stem cells, Tβ4 did not significantly change smad.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changesmad downstream protein
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    No significant difference was found in BMP2/BMP4 or their downstream protein smad.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  13. 13LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 remarkably decreased Wnt3.

    In plain wordsIn mouse stem cells, Tβ4 strongly lowered Wnt3.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseWnt3 expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant β-catenin.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  14. 14LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 remarkably decreased Wnt11.

    In plain wordsIn mouse stem cells, Tβ4 strongly lowered Wnt11.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseWnt11 expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant β-catenin.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  15. 15LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, Tβ4 upregulated Tcf3.

    In plain wordsIn mouse stem cells, Tβ4 raised Tcf3.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseTcf3 expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant β-catenin.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  16. 16LabIn vitro (cells)Mechanism

    In mouse embryonic stem cells, β-catenin stayed constant with Tβ4.

    In plain wordsIn mouse stem cells, Tβ4 did not change β-catenin.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeβ-catenin expression
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wnt3 and Wnt11 were remarkably decreased by Tβ4 with upregulation of Tcf3 and constant β-catenin.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  17. 17LabIn vitro (cells)Mechanism

    During differentiation of mouse embryonic stem cells, Tβ4 did not change expression of the cardiovascular marker α-MHC.

    In plain wordsIn mouse stem cells, Tβ4 did not change a heart-cell marker, α-MHC.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeα-MHC expression during differentiation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Under mESCs differentiation, Tβ4 treatment did not change the expression of cardiovascular cell markers α-MHC, PECAM, and α-SMA.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  18. 18LabIn vitro (cells)Mechanism

    During differentiation of mouse embryonic stem cells, Tβ4 did not change expression of the cardiovascular marker PECAM.

    In plain wordsIn mouse stem cells, Tβ4 did not change a blood-vessel-cell marker, PECAM.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changePECAM expression during differentiation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Under mESCs differentiation, Tβ4 treatment did not change the expression of cardiovascular cell markers α-MHC, PECAM, and α-SMA.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  19. 19LabIn vitro (cells)Mechanism

    During differentiation of mouse embryonic stem cells, Tβ4 did not change expression of the cardiovascular marker α-SMA.

    In plain wordsIn mouse stem cells, Tβ4 did not change a smooth-muscle marker, α-SMA.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeα-SMA expression during differentiation
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Under mESCs differentiation, Tβ4 treatment did not change the expression of cardiovascular cell markers α-MHC, PECAM, and α-SMA.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  20. 20LabIn vitro (cells)Mechanism

    The electrophysiological properties of mouse embryonic stem cell-derived cardiomyocytes were not affected by Tβ4.

    In plain wordsIn mouse stem cell-derived heart cells, Tβ4 did not change electrical properties.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeElectrophysiological properties of mESC-derived cardiomyocytes
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Neither the electrophysiological properties of mESCs-derived cardiomyocytes nor the hormonal regulation by Iso/Cch was affected by Tβ4.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.
  21. 21LabIn vitro (cells)Mechanism

    The hormonal regulation by Iso/Cch of mouse embryonic stem cell-derived cardiomyocytes was not affected by Tβ4.

    In plain wordsIn mouse stem cell-derived heart cells, Tβ4 did not change the response to Iso and Cch.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeHormonal regulation by Iso/Cch in mESC-derived cardiomyocytes
    Study

    mousen: not reported in source

    murine embryonic stem cells (mESCs)
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Neither the electrophysiological properties of mESCs-derived cardiomyocytes nor the hormonal regulation by Iso/Cch was affected by Tβ4.
    1 context sentence
    Summary · context
    Target genes during mESCs proliferation and differentiation were detected by real-time PCR or Western blotting, and patch clamp was applied to characterize the mESCs-derived cardiomyocytes.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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