Kisspeptin Activates Ankrd 26 Gene Expression in Migrating Embryonic GnRH Neurons.

Soga T, Lim WL, Khoo AS, Parhar IS

Frontiers in endocrinology1 Mar 2016Cited by 3 (Europe PMC)

PMID26973595PMCIDPMC4771921DOI10.3389/fendo.2016.00015PepBankPUB-26973595

Summary

Kisspeptin, a newly discovered neuropeptide, regulates gonadotropin-releasing hormone (GnRH). Kisspeptins are a large RF-amide family of peptides. The kisspeptin coded by KiSS-1 gene is a 145-amino acid protein that is cleaved to C-terminal peptide kisspeptin-10. G-protein-coupled receptor 54 (GPR54) has been identified as a kisspeptin receptor, and it is expressed in GnRH neurons and in a variety of cancer cells. In this study, enhanced green fluorescent protein (EGFP) labeled GnRH cells with migratory properties, which express GPR54, served as a model to study the effects of kisspeptin on cell migration. We monitored EGFP-GnRH neuronal migration in brain slide culture of embryonic day 14 transgenic rat by live cell imaging system and studied the effects of kisspeptin-10 (1 nM) treatment for 36 h on GnRH migration. Furthermore, to determine kisspeptin-induced molecular pathways related with apoptosis and cytoskeletal changes during neuronal migration, we studied the expression levels of candidate genes in laser-captured EGFP-GnRH neurons by real-time PCR. We found that there was no change in the expression level of genes related to cell proliferation and apoptosis. The expression of ankyrin repeat domain-containing protein (ankrd) 26 in EGFP-GnRH neurons was upregulated by the exposure to kisspeptin. These studies suggest that ankrd 26 gene plays an unidentified role in regulating neuronal movement mediated by kisspeptin-GPR54 signaling, which could be a potential pathway to suppress cell migration.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In embryonic rat GnRH neurons in brain slice culture, kisspeptin exposure did not change expression of genes related to cell proliferation and apoptosis.
In plain wordsIn rat brain slices, kisspeptin did not change the activity of genes linked to cell growth and cell death in GnRH neurons.
Peptide
Kisspeptin-10
Outcome
No significant changeExpression of proliferation and apoptosis genes in GnRH neurons
Study
Rat (embryonic day 14, transgenic)n: not reported in source
Brain slice culture with EGFP-GnRH neurons
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
1 nM · 36 hAs given in this study (Rat (embryonic day 14, transgenic)).
Source sentences
Summary
We found that there was no change in the expression level of genes related to cell proliferation and apoptosis.
1 context sentence(design, population, supporting numbers)
Summary · context
We monitored EGFP-GnRH neuronal migration in brain slide culture of embryonic day 14 transgenic rat by live cell imaging system and studied the effects of kisspeptin-10 (1 nM) treatment for 36 h on GnRH migration.
Show in the study text Report a problemFND-2697359501
02LabEx vivo tissueMechanism
In embryonic rat GnRH neurons in brain slice culture, kisspeptin exposure upregulated ankrd26 expression.
In plain wordsIn rat brain slices, kisspeptin raised the activity of the ankrd26 gene in GnRH neurons.
Peptide
Kisspeptin-10
Outcome
IncreaseAnkrd26 expression in GnRH neurons
Study
Rat (embryonic day 14, transgenic)n: not reported in source
Brain slice culture with EGFP-GnRH neurons
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
1 nM · 36 hAs given in this study (Rat (embryonic day 14, transgenic)).
Source sentences
Summary
The expression of ankyrin repeat domain-containing protein (ankrd) 26 in EGFP-GnRH neurons was upregulated by the exposure to kisspeptin.
1 context sentence(design, population, supporting numbers)
Summary · context
We monitored EGFP-GnRH neuronal migration in brain slide culture of embryonic day 14 transgenic rat by live cell imaging system and studied the effects of kisspeptin-10 (1 nM) treatment for 36 h on GnRH migration.
Show in the study text Report a problemFND-2697359502

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Version history

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Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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