Development and Validation of a Sensitive Enzymeimmunoassay for Determination of Plasma Metastin in Mithun (Bos frontalis).

Mondal M, Karunakaran M, Baruah KK

Journal of immunoassay & immunochemistry1 Jan 2016Cited by 8 (Europe PMC)

PMID26599603DOI10.1080/15321819.2015.1120745PepBankPUB-26599603

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
Plasma metastin was higher in early pregnant mithun cows than in non-pregnant cows.
In plain wordsIn mithun cows, the body's own metastin level in plasma was higher in early pregnancy than in non-pregnant cows.
Peptide
Kisspeptin-10
Outcome
IncreasePlasma metastin in early pregnant versus non-pregnant cows
Study
Mithun (Bos frontalis)n = 19
Plasma and follicular fluid samples
Comparator
Non-pregnant mithun cows
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Plasma metastin levels were much higher (P < 0.001) in early pregnant than non-pregnant cows.
1 context sentence(design, population, supporting numbers)
Summary · context
For the biological validation of the assay, metastin was measured in (a) blood samples collected from 12 pregnant mithun cows during different stages of pregnancy, (b) in blood from seven early pregnant and 12 non-pregnant mithuns, and (c) in follicular fluid obtained from different types of follicle.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2659960303
02AnimalEndogenous levelAnimal study (in vivo)Other
In pregnant mithun cows, the body's own plasma metastin rose from the first to the last trimester of pregnancy.
In plain wordsIn pregnant mithun cows, the body's own metastin level in plasma rose from early to late pregnancy.
Peptide
Kisspeptin-10
Outcome
IncreasePlasma metastin during pregnancy
Study
Mithun (Bos frontalis)n = 12
Plasma and follicular fluid samples
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
It was found that the plasma metastin concentrations increased (P < 0.001) from first through last trimester of pregnancy.
1 context sentence(design, population, supporting numbers)
Summary · context
For the biological validation of the assay, metastin was measured in (a) blood samples collected from 12 pregnant mithun cows during different stages of pregnancy, (b) in blood from seven early pregnant and 12 non-pregnant mithuns, and (c) in follicular fluid obtained from different types of follicle.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2659960302
03AnimalEndogenous levelAnimal study (in vivo)Other
Metastin concentration in follicular fluid of mithun increased as follicles grew, with the highest levels in preovulatory follicles.
In plain wordsIn mithun, the body's own metastin level in follicular fluid rose as follicles grew and was highest in preovulatory follicles.
Peptide
Kisspeptin-10
Outcome
IncreaseFollicular fluid metastin by follicle growth
Study
Mithun (Bos frontalis)n: not reported in source
Plasma and follicular fluid samples
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Follicular fluid metastin concentrations were found to increase (P < 0.001) as the follicles grow and the highest levels were recorded in preovulatory follicles.
1 context sentence(design, population, supporting numbers)
Summary · context
For the biological validation of the assay, metastin was measured in (a) blood samples collected from 12 pregnant mithun cows during different stages of pregnancy, (b) in blood from seven early pregnant and 12 non-pregnant mithuns, and (c) in follicular fluid obtained from different types of follicle.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2659960304

Studies that did not test the peptide alone1

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

04AnimalNot a test of Kisspeptin-10 aloneAnimal study (in vivo)Other
An enzyme immunoassay for metastin (kisspeptin‑10) in mithun plasma had a lowest detection limit of 0.07 ng/mL.
In plain wordsA new lab test could detect metastin (kisspeptin‑10) in mithun plasma down to 0.07 ng/mL.
Peptide
Kisspeptin-10
Outcome
Not applicableEnzyme immunoassay detection limit for metastin
Study
Mithun (Bos frontalis)n: not reported in source
Plasma and follicular fluid samples
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Lowest detection limit
0.07 ng/mL
mithun plasma
Source sentences
Summary
The lowest detection limit was 0.07 ng/mL plasma.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, the objective of this study was to develop and validate a simple and sufficiently sensitive enzyme immunoassay (EIA) for metastin determination in mithun plasma using the biotin-streptavidin amplification system and second antibody coating technique.
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.
Report a problemFND-2659960301

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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