In KISS1R-transfected CHO cells, RFRP-3 did not alter the responses to kisspeptin‑10 or RF9; kisspeptin‑10 served as a reference agonist for testing RF9.
In plain wordsIn cells, RFRP-3 did not change how the cells responded to kisspeptin‑10.
- Peptide
- Kisspeptin-10
- Outcome
- No significant changeKisspeptin-10 responses with RFRP-3 (inositol phosphate, pERK)
- Study
Chinese hamstern: not reported in source
CHO cells stably transfected with KISS1R- Outcome group
- Receptor activity and mechanism · Reproductive and sexual health
Source sentences
RFRP-3, the putative endogenous ligand for NPFFR1, did not stimulate inositol phosphate accumulation or pERK, nor did it alter responses to of kisspeptin-10 or RF9.
1 context sentence(design, population, supporting numbers)
Using Chinese Hamster Ovary cells stably transfected with KISS1R, we show that RF9 binds specifically to KISS1R, with a Kd of 1.6 × 10(-5)M, and stimulates an increase in intracellular calcium and inositol phosphate accumulation in a KISS1R-dependent manner, with EC50 values of 3.0 × 10(-6)M and 1.6…
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.