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Study · In vitro (cells) · rat · 2015

7 results

Melanocortin 4 receptor activates ERK-cFos pathway to increase brain-derived neurotrophic factor expression in rat astrocytes and hypothalamus.

Ramírez D, Saba J, Carniglia L, Durand D, Lasaga M, Caruso C

Molecular and cellular endocrinology16 Apr 2015Cited by 36 (Europe PMC)

PMID25892444 (opens in a new tab)DOI10.1016/j.mce.2015.04.008 (opens in a new tab)PepBankPUB-25892444
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, NDP-MSH at 1 µM for 1 h increased BDNF expression.

    In plain wordsIn rat brain cells grown in a dish, NDP-MSH raised the level of BDNF, a nerve growth protein.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseBDNF expression in astrocytes
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    1 µM · 1 hAs given in this study (rat).

    Measurements

    Concentration
    1 µM
    rat astrocytes, 1 h

    Source sentences

    Summary
    Rat cultured astrocytes treated with NDP-MSH 1 µM for 1 h showed increased BDNF expression.
  2. 02LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, inhibition of ERK and RSK prevented the NDP-MSH-induced increase in BDNF expression.

    In plain wordsIn rat brain cells grown in a dish, blocking ERK or RSK stopped NDP-MSH from raising BDNF.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableERK/RSK dependence of NDP-MSH-induced BDNF expression
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Inhibition of extracellular signal-regulated kinase (ERK) and ribosomal p90 S6 kinase (RSK), an ERK substrate, but not of p38 or JNK, prevented the increase in BDNF expression induced by NDP-MSH.
  3. 03LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, inhibition of p38 or JNK did not prevent the NDP-MSH-induced increase in BDNF expression.

    In plain wordsIn rat brain cells grown in a dish, blocking p38 or JNK did not stop NDP-MSH from raising BDNF.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changep38/JNK dependence of NDP-MSH-induced BDNF expression
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Inhibition of extracellular signal-regulated kinase (ERK) and ribosomal p90 S6 kinase (RSK), an ERK substrate, but not of p38 or JNK, prevented the increase in BDNF expression induced by NDP-MSH.
  4. 04LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, MC4R activation increased cFos expression, a target of both ERK and RSK.

    In plain wordsIn rat brain cells grown in a dish, activating the MC4R receptor raised cFos, a protein downstream of ERK and RSK.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasecFos expression after MC4R activation
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Activation of MC4R increased cFos expression, a target of both ERK and RSK.
  5. 05LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, ERK activation by MC4R involves cAMP, PI3K and the tyrosine kinase Src.

    In plain wordsIn rat brain cells grown in a dish, MC4R activates ERK through cAMP, PI3K and Src.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableSignalling steps in MC4R-induced ERK activation
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    ERK activation by MC4R involves cAMP, phosphoinositide-3 kinase (PI3K) and the non receptor tyrosine kinase, Src.
  6. 06LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, PI3K inhibition abolished NDP-MSH-induced BDNF expression.

    In plain wordsIn rat brain cells grown in a dish, blocking PI3K stopped NDP-MSH from raising BDNF.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicablePI3K dependence of NDP-MSH-induced BDNF expression
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Both PI3K and Src inhibition abolished NDP-MSH-induced BDNF expression.
  7. 07LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, Src inhibition abolished NDP-MSH-induced BDNF expression.

    In plain wordsIn rat brain cells grown in a dish, blocking Src stopped NDP-MSH from raising BDNF.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableSrc dependence of NDP-MSH-induced BDNF expression
    Study

    ratn: not reported in source

    cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Both PI3K and Src inhibition abolished NDP-MSH-induced BDNF expression.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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