Skip to content

Study · Ex vivo tissue · mouse · 2015

6 results

Antioxidant treatments do not improve force recovery after fatiguing stimulation of mouse skeletal muscle fibres.

Cheng AJ, Bruton JD, Lanner JT, Westerblad H

The Journal of physiology11 Dec 2014Cited by 74 (Europe PMC)

PMID25630265 (opens in a new tab)DOI10.1113/jphysiol.2014.279398 (opens in a new tab)PepBankPUB-25630265
Publisher pagePubMedAsk about this study
Jump to 6 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In isolated mouse fast-twitch fibres, SS‑31 did not change the fatigue-induced fall in free cytosolic Ca2+ and peak force compared with control.

    In plain wordsIn mouse muscle fibres in the lab, SS‑31 did not change how much calcium and force fell during fatigue, compared with control.

    Peptide
    Elamipretide (SS-31)
    Outcome
    No significant changeFatigue-induced fall in [Ca2+]i and peak force
    Study

    mousen: not reported in source

    Mechanically isolated fast-twitch muscle fibres
    Comparator
    Control (standard Tyrode solution)
    Outcome group
    Exercise and physical function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Spatially and temporally averaged [Ca(2+) ]i and peak force were reduced by ∼20% and ∼70% at the end of fatiguing stimulation, respectively, with no marked differences between groups.
    2 context sentences
    Summary · context
    Mechanically isolated mouse fast-twitch fibres were fatigued by sixty 150 ms, 70 Hz tetani given every 1 s.
    Summary · context
    Experiments were performed in standard Tyrode solution (control) or in the presence of: NADPH oxidase (NOX) 2 inhibitor (gp91ds-tat); NOX4 inhibitor (GKT137831); mitochondria-targeted antioxidant (SS-31); nitric oxide synthase (NOS) inhibitor (l-NAME); the general antioxidant N-acetylcysteine (NAC);…
  2. 02LabEx vivo tissueEfficacy outcome

    In isolated mouse fast-twitch fibres, SS‑31 did not change prolonged low-frequency force depression 30 min after fatigue; it was similar in all groups.

    In plain wordsIn mouse muscle fibres in the lab, force loss 30 minutes after fatigue was similar with SS‑31 and in control fibres.

    Peptide
    Elamipretide (SS-31)
    Outcome
    No significant changeProlonged low-frequency force depression (PLFFD) after fatigue
    Study

    mousen: not reported in source

    Mechanically isolated fast-twitch muscle fibres
    Comparator
    Control (standard Tyrode solution)
    Outcome group
    Exercise and physical function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PLFFD was similar in all groups, with 30 Hz force being decreased by ∼60% at 30 min of recovery.
    2 context sentences
    Summary · context
    Mechanically isolated mouse fast-twitch fibres were fatigued by sixty 150 ms, 70 Hz tetani given every 1 s.
    Summary · context
    Experiments were performed in standard Tyrode solution (control) or in the presence of: NADPH oxidase (NOX) 2 inhibitor (gp91ds-tat); NOX4 inhibitor (GKT137831); mitochondria-targeted antioxidant (SS-31); nitric oxide synthase (NOS) inhibitor (l-NAME); the general antioxidant N-acetylcysteine (NAC);…
  3. 03LabEx vivo tissueMechanism

    In isolated mouse fast-twitch fibres exposed to SS‑31 (or an anti-ROS/RNS cocktail), tetanic [Ca2+]i was not decreased during recovery, unlike in control fibres.

    In plain wordsIn mouse muscle fibres in the lab, SS‑31 kept calcium release from falling during recovery from fatigue, unlike control fibres.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseTetanic [Ca2+]i during recovery from fatigue
    Study

    mousen: not reported in source

    Mechanically isolated fast-twitch muscle fibres
    Comparator
    Control (standard Tyrode solution)
    Outcome group
    Exercise and physical function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Conversely, in fibres exposed to SS-31 or the anti ROS/RNS cocktail, tetanic [Ca(2+) ]i was not decreased during recovery so PLFFD was only caused by decreased myofibrillar Ca(2+) sensitivity.
    3 context sentences
    Summary · context
    PLFFD was mostly due to decreased tetanic [Ca(2+) ]i in control fibres and in the presence of NOX2 or NOX4 inhibitors.
    Summary · context
    Mechanically isolated mouse fast-twitch fibres were fatigued by sixty 150 ms, 70 Hz tetani given every 1 s.
    Summary · context
    Experiments were performed in standard Tyrode solution (control) or in the presence of: NADPH oxidase (NOX) 2 inhibitor (gp91ds-tat); NOX4 inhibitor (GKT137831); mitochondria-targeted antioxidant (SS-31); nitric oxide synthase (NOS) inhibitor (l-NAME); the general antioxidant N-acetylcysteine (NAC);…
  4. 04LabEx vivo tissueMechanism

    In isolated mouse fast-twitch fibres exposed to SS‑31 (or an anti-ROS/RNS cocktail), force depression was caused only by decreased myofibrillar Ca2+ sensitivity.

    In plain wordsIn mouse muscle fibres in the lab, with SS‑31 the lasting force loss came only from lower calcium sensitivity of the muscle filaments.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseMyofibrillar Ca2+ sensitivity during recovery from fatigue
    Study

    mousen: not reported in source

    Mechanically isolated fast-twitch muscle fibres
    Comparator
    Control (standard Tyrode solution)
    Outcome group
    Exercise and physical function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Conversely, in fibres exposed to SS-31 or the anti ROS/RNS cocktail, tetanic [Ca(2+) ]i was not decreased during recovery so PLFFD was only caused by decreased myofibrillar Ca(2+) sensitivity.
    2 context sentences
    Summary · context
    Mechanically isolated mouse fast-twitch fibres were fatigued by sixty 150 ms, 70 Hz tetani given every 1 s.
    Summary · context
    Experiments were performed in standard Tyrode solution (control) or in the presence of: NADPH oxidase (NOX) 2 inhibitor (gp91ds-tat); NOX4 inhibitor (GKT137831); mitochondria-targeted antioxidant (SS-31); nitric oxide synthase (NOS) inhibitor (l-NAME); the general antioxidant N-acetylcysteine (NAC);…

Studies that did not test the peptide alone2

In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Elamipretide (SS-31).

  1. 05LabNot a test of Elamipretide (SS-31) aloneEx vivo tissueSafety outcome

    In isolated mouse fast-twitch fibres, a cocktail of SS‑31, l-NAME and NAC increased resting free cytosolic Ca2+.

    In plain wordsIn mouse muscle fibres in the lab, a mix of three antioxidant compounds including SS‑31 raised resting calcium.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseResting [Ca2+]i with antioxidant cocktail
    Study

    mousen: not reported in source

    Mechanically isolated fast-twitch muscle fibres
    Comparator
    Control (standard Tyrode solution)
    Outcome group
    Exercise and physical function · Musculoskeletal

    Source sentences

    Summary
    The cocktail also increased resting [Ca(2+) ]i and ultimately caused cell death.
    2 context sentences
    Summary · context
    Mechanically isolated mouse fast-twitch fibres were fatigued by sixty 150 ms, 70 Hz tetani given every 1 s.
    Summary · context
    Experiments were performed in standard Tyrode solution (control) or in the presence of: NADPH oxidase (NOX) 2 inhibitor (gp91ds-tat); NOX4 inhibitor (GKT137831); mitochondria-targeted antioxidant (SS-31); nitric oxide synthase (NOS) inhibitor (l-NAME); the general antioxidant N-acetylcysteine (NAC);…

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

  2. 06LabNot a test of Elamipretide (SS-31) aloneEx vivo tissueSafety outcome

    In isolated mouse fast-twitch fibres, a cocktail of SS‑31, l-NAME and NAC ultimately caused cell death.

    In plain wordsIn mouse muscle fibres in the lab, a mix of three antioxidant compounds including SS‑31 ended in cell death.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseCell death with antioxidant cocktail
    Study

    mousen: not reported in source

    Mechanically isolated fast-twitch muscle fibres
    Comparator
    Control (standard Tyrode solution)
    Outcome group
    Exercise and physical function · Musculoskeletal

    Source sentences

    Summary
    The cocktail also increased resting [Ca(2+) ]i and ultimately caused cell death.
    2 context sentences
    Summary · context
    Mechanically isolated mouse fast-twitch fibres were fatigued by sixty 150 ms, 70 Hz tetani given every 1 s.
    Summary · context
    Experiments were performed in standard Tyrode solution (control) or in the presence of: NADPH oxidase (NOX) 2 inhibitor (gp91ds-tat); NOX4 inhibitor (GKT137831); mitochondria-targeted antioxidant (SS-31); nitric oxide synthase (NOS) inhibitor (l-NAME); the general antioxidant N-acetylcysteine (NAC);…

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.