Kisspeptin drives germ cell progression in the anuran amphibian Pelophylax esculentus: a study carried out in ex vivo testes.

Chianese R, Ciaramella V, Fasano S, Pierantoni R, Meccariello R

General and comparative endocrinology18 Nov 2014Cited by 31 (Europe PMC)

PMID25452028DOI10.1016/j.ygcen.2014.11.008PepBankPUB-25452028

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In ex vivo frog testes, 4 h of Kp-10 incubation upregulated expression of pcna, a proliferation marker.
In plain wordsIn frog testes in a lab dish, kisspeptin‑10 raised activity of pcna, a cell growth marker, after 4 hours.
Peptide
Kisspeptin-10
Outcome
IncreasePcna expression at 4 h
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-9 to 10^-6 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
All selected markers were up regulated at 4h Kp-10 incubation.
1 context sentence(design, population, supporting numbers)
Summary · context
In the pre-reproductive period, dose response curve to assess the effects of Kisspeptin-10 (Kp-10) was carried out in vitro (dose range: 10(-9)-10(-6)M; incubation times: 1 and 4h)
Report a problemFND-2545202801
02LabEx vivo tissueMechanism
In ex vivo frog testes, 4 h of Kp-10 incubation upregulated expression of estrogen receptor beta.
In plain wordsIn frog testes in a lab dish, kisspeptin‑10 raised activity of the estrogen receptor beta gene after 4 hours.
Peptide
Kisspeptin-10
Outcome
IncreaseEstrogen receptor beta expression at 4 h
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-9 to 10^-6 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
All selected markers were up regulated at 4h Kp-10 incubation.
1 context sentence(design, population, supporting numbers)
Summary · context
In the pre-reproductive period, dose response curve to assess the effects of Kisspeptin-10 (Kp-10) was carried out in vitro (dose range: 10(-9)-10(-6)M; incubation times: 1 and 4h)
Report a problemFND-2545202802
03LabEx vivo tissueMechanism
In ex vivo frog testes, 4 h of Kp-10 incubation upregulated expression of the Gnrh system genes (gnrh1, gnrh2, gnrhr1, r2, r3).
In plain wordsIn frog testes in a lab dish, kisspeptin‑10 raised activity of the GnRH system genes after 4 hours.
Peptide
Kisspeptin-10
Outcome
IncreaseGnrh system gene expression at 4 h
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-9 to 10^-6 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
All selected markers were up regulated at 4h Kp-10 incubation.
1 context sentence(design, population, supporting numbers)
Summary · context
In the pre-reproductive period, dose response curve to assess the effects of Kisspeptin-10 (Kp-10) was carried out in vitro (dose range: 10(-9)-10(-6)M; incubation times: 1 and 4h)
Report a problemFND-2545202803
04LabEx vivo tissueEfficacy outcome
In ex vivo frog testes, Kp-10 increased mitotic activity on histological analysis.
In plain wordsIn frog testes in a lab dish, kisspeptin‑10 increased cell division in germ cells.
Peptide
Kisspeptin-10
Outcome
IncreaseMitotic activity of germ cells
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-9 to 10^-6 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
Histological analysis also proved the increase of mitotic activity and the progression of spermatogenesis.
1 context sentence(design, population, supporting numbers)
Summary · context
In the pre-reproductive period, dose response curve to assess the effects of Kisspeptin-10 (Kp-10) was carried out in vitro (dose range: 10(-9)-10(-6)M; incubation times: 1 and 4h)
Report a problemFND-2545202804
05LabEx vivo tissueEfficacy outcome
In ex vivo frog testes, Kp-10 promoted progression of spermatogenesis on histological analysis.
In plain wordsIn frog testes in a lab dish, kisspeptin‑10 pushed sperm cell development forward.
Peptide
Kisspeptin-10
Outcome
IncreaseProgression of spermatogenesis
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-9 to 10^-6 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
Histological analysis also proved the increase of mitotic activity and the progression of spermatogenesis.
1 context sentence(design, population, supporting numbers)
Summary · context
In the pre-reproductive period, dose response curve to assess the effects of Kisspeptin-10 (Kp-10) was carried out in vitro (dose range: 10(-9)-10(-6)M; incubation times: 1 and 4h)
Report a problemFND-2545202805
06LabEx vivo tissueMechanism
In ex vivo frog testes in the reproductive period, the antagonist Kp-234 completely counteracted the effects of Kp-10.
In plain wordsIn frog testes in a lab dish, a kisspeptin blocker fully cancelled the effects of kisspeptin‑10.
Peptide
Kisspeptin-10
Outcome
No significant changeKp-10 effects with kisspeptin antagonist Kp-234
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Comparator
Kp-10 plus Kp-234 (10^-6 M)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-7 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
In the reproductive period, testes were incubated for 1 and 4h with Kp-10 (10(-7)M) or Kp-10 (10(-7)M)+kisspeptin antagonist [Kp-234 (10(-6)M)]. Results obtained in the pre-reproductive period were confirmed and Kp-234 completely counteracted Kp-10 effects.
1 context sentence(design, population, supporting numbers)
Summary · context
In the pre-reproductive period, dose response curve to assess the effects of Kisspeptin-10 (Kp-10) was carried out in vitro (dose range: 10(-9)-10(-6)M; incubation times: 1 and 4h)
Report a problemFND-2545202806
07LabEx vivo tissueMechanism
In ex vivo frog testes in the reproductive period, incubation with Kp-10 confirmed the effects seen in the pre-reproductive period.
In plain wordsIn frog testes in a lab dish during the breeding season, kisspeptin‑10 had the same effects as seen before the breeding season.
Peptide
Kisspeptin-10
Outcome
IncreaseKp-10 effects in the reproductive period
Study
Pelophylax esculentus (frog)n: not reported in source
Ex vivo testes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10^-7 M · 1 and 4 hAs given in this study (Pelophylax esculentus (frog)).
Source sentences
Summary
Results obtained in the pre-reproductive period were confirmed and Kp-234 completely counteracted Kp-10 effects.
1 context sentence(design, population, supporting numbers)
Summary · context
In the reproductive period, testes were incubated for 1 and 4h with Kp-10 (10(-7)M) or Kp-10 (10(-7)M)+kisspeptin antagonist [Kp-234 (10(-6)M)].
Report a problemFND-2545202807

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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Kisspeptin drives germ cell progression in the anuran amphibian Pelophylax esculentus: a study carried out in ex vivo testes.

Summary

Results from this study7

Version history

Subjects

Publication types

MeSH terms

Keywords