Protection effect of [Gly14]-Humanin from apoptosis induced by high glucose in human umbilical vein endothelial cells.

Xie Y, Liu ZH, Li XY, Zhou YD, Xu X, Hu LF, Zhang YL, Liu CF

Diabetes research and clinical practice16 Oct 2014Cited by 20 (Europe PMC)

PMID25451915DOI10.1016/j.diabres.2014.09.020PepBankPUB-25451915

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In HUVECs exposed to high glucose, HNG pretreatment significantly increased cell viability.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment improved cell survival under high sugar, compared with high sugar alone.
Peptide
Humanin
Outcome
IncreaseHUVEC cell viability under high glucose
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P<0.001), reduced nuclear fluorescence of HUVECs (P<0.05), the levels of cleaved PARP (P<0.05), ROS formation (P<0.05) and the ratio of bax/bcl-2 (P<0.05) compared with treatment with HG for 72h.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191501
02LabIn vitro (cells)Efficacy outcome
In HUVECs exposed to high glucose, HNG pretreatment reduced nuclear fluorescence.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment reduced signs of damaged cell nuclei under high sugar.
Peptide
Humanin
Outcome
DecreaseNuclear fluorescence of HUVECs (Hoechst staining)
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P<0.001), reduced nuclear fluorescence of HUVECs (P<0.05), the levels of cleaved PARP (P<0.05), ROS formation (P<0.05) and the ratio of bax/bcl-2 (P<0.05) compared with treatment with HG for 72h.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191502
03LabIn vitro (cells)Mechanism
In HUVECs exposed to high glucose, HNG pretreatment reduced the level of cleaved PARP.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment lowered a cell-death marker, cleaved PARP, under high sugar.
Peptide
Humanin
Outcome
DecreaseCleaved PARP level
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P<0.001), reduced nuclear fluorescence of HUVECs (P<0.05), the levels of cleaved PARP (P<0.05), ROS formation (P<0.05) and the ratio of bax/bcl-2 (P<0.05) compared with treatment with HG for 72h.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191503
04LabIn vitro (cells)Mechanism
In HUVECs exposed to high glucose, HNG pretreatment reduced ROS formation.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment reduced harmful oxygen molecules formed under high sugar.
Peptide
Humanin
Outcome
DecreaseReactive oxygen species formation
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P<0.001), reduced nuclear fluorescence of HUVECs (P<0.05), the levels of cleaved PARP (P<0.05), ROS formation (P<0.05) and the ratio of bax/bcl-2 (P<0.05) compared with treatment with HG for 72h.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191504
05LabIn vitro (cells)Mechanism
In HUVECs exposed to high glucose, HNG pretreatment reduced the bax/bcl-2 ratio.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment shifted the balance of cell-death proteins away from death under high sugar.
Peptide
Humanin
Outcome
DecreaseBax/bcl-2 protein ratio
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Compared with treatment with HG 72h, pretreatment with HNG for 3h significantly increased cell viability (P<0.001), reduced nuclear fluorescence of HUVECs (P<0.05), the levels of cleaved PARP (P<0.05), ROS formation (P<0.05) and the ratio of bax/bcl-2 (P<0.05) compared with treatment with HG for 72h.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191505
06LabIn vitro (cells)Mechanism
In HUVECs, the mRNA level of bax was reduced after HNG pretreatment.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment lowered the messenger RNA of the cell-death gene bax.
Peptide
Humanin
Outcome
DecreaseBax mRNA level
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Quantitative Real-Time PCR showed that mRNA level of bax reduced (P<0.05) and mRNA level of bcl-2 increased (P<0.05) after pretreatment with HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191506
07LabIn vitro (cells)Mechanism
In HUVECs, the mRNA level of bcl-2 was increased after HNG pretreatment.
In plain wordsIn human blood-vessel cells in a dish, HNG pretreatment raised the messenger RNA of the survival gene bcl-2.
Peptide
Humanin
Outcome
IncreaseBcl-2 mRNA level
Study
humann: not reported in source
HUVECs under high glucose
Comparator
High glucose for 72 h without HNG
Outcome group
Cell viability and death · Cardiovascular
Studied dose
Dose not stated · 3 h pretreatmentAs given in this study (human).
Source sentences
Summary
Quantitative Real-Time PCR showed that mRNA level of bax reduced (P<0.05) and mRNA level of bcl-2 increased (P<0.05) after pretreatment with HNG.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we sought to investigate the protective effect of [Gly14]-Humanin (HNG) in high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs).
Report a problemFND-2545191507

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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Protection effect of [Gly14]-Humanin from apoptosis induced by high glucose in human umbilical vein endothelial cells.

Summary

Results from this study7

Version history

Subjects

Publication types

MeSH terms

Keywords