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Study · Animal study (in vivo) · mouse · 2014

5 results

Extending the time window of mammalian heart regeneration by thymosin beta 4.

Rui L, Yu N, Hong L, Feng H, Chunyong H, Jian M, Zhe Z, Shengshou H

Journal of cellular and molecular medicine6 Oct 2014Cited by 32 (Europe PMC)

PMID25284727 (opens in a new tab)PMCIDPMC4302647 (opens in a new tab)DOI10.1111/jcmm.12421 (opens in a new tab)PepBankPUB-25284727
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Summary

Recent studies demonstrated that the heart of 1-day-old neonatal mice could regenerate, with Wt1(+) EPDCs migrating into myocardial regions after partial surgical resection, but this capacity was lost by 7 days of age. By treatment with Tβ4 to maintain Wt1 expression and retain the migrating feature of EPDCs in neonatal mice, we explored the possibility of restoring the cardiac regeneration potential of mice. We intraperitoneally injected Tβ4 into 1-day-old mice on daily basis and then apical resection was performed on the mice 7 days later. Twenty one days after the resection, morphological analysis revealed that the Tβ4-treated mice regenerated the resected ventricular apex, while the mice in PBS control group developed significant fibrosis without apical regeneration. The Tβ4-treated mice had significantly better ventricular ejection fraction and fractional shortening than controls. During the process of regeneration, Wt1(+) EPDCs migrated into myocardial region and some of them expressed Islet1 and the markers for mature cardiomyocytes, such as cTnT and SαA. These characteristics of Wt1(+) EPDCs were also seen in the heart regeneration of mice subjected to apical resection 1 day after birth. Tβ4 has no essential effect on cell cycle activity as no disruption of actin filaments was observed in Tβ4-treated hearts. These results revealed that the cardiac regeneration potential of neonatal mice could be extended to the 7th post-natal day by Tβ4 and Wt1(+) EPDCs mobilization might play an important role in the extension.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In neonatal mice treated with Tβ4 before apical resection, the resected ventricular apex regenerated, while PBS controls developed significant fibrosis without apical regeneration.

    In plain wordsIn mice, thymosin beta‑4 treatment let the cut heart tip regrow, while control mice formed scar tissue instead.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseRegeneration of resected ventricular apex
    Study

    mousen: not reported in source

    neonatal mice with apical resection
    Comparator
    PBS control
    Outcome group
    Heart repair and regeneration · Cardiovascular
    Studied dose
    Dose not stated · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    Twenty one days after the resection, morphological analysis revealed that the Tβ4-treated mice regenerated the resected ventricular apex, while the mice in PBS control group developed significant fibrosis without apical regeneration.
    1 context sentence
    Summary · context
    We intraperitoneally injected Tβ4 into 1-day-old mice on daily basis and then apical resection was performed on the mice 7 days later.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In neonatal mice after apical resection, Tβ4 treatment gave better ventricular ejection fraction than in controls.

    In plain wordsIn mice, thymosin beta‑4 treatment gave better heart pumping (ejection fraction) than in control mice.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseVentricular ejection fraction
    Study

    mousen: not reported in source

    neonatal mice with apical resection
    Comparator
    PBS control
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    The Tβ4-treated mice had significantly better ventricular ejection fraction and fractional shortening than controls.
    1 context sentence
    Summary · context
    We intraperitoneally injected Tβ4 into 1-day-old mice on daily basis and then apical resection was performed on the mice 7 days later.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In neonatal mice after apical resection, Tβ4 treatment gave better fractional shortening than in controls.

    In plain wordsIn mice, thymosin beta‑4 treatment gave better heart muscle shortening than in control mice.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseFractional shortening
    Study

    mousen: not reported in source

    neonatal mice with apical resection
    Comparator
    PBS control
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    The Tβ4-treated mice had significantly better ventricular ejection fraction and fractional shortening than controls.
    1 context sentence
    Summary · context
    We intraperitoneally injected Tβ4 into 1-day-old mice on daily basis and then apical resection was performed on the mice 7 days later.
  4. 04AnimalAnimal study (in vivo)Mechanism

    During regeneration in Tβ4-treated neonatal mice, Wt1(+) EPDCs migrated into the myocardial region and some expressed Islet1 and mature cardiomyocyte markers.

    In plain wordsIn mice, thymosin beta‑4 treatment was followed by migration of heart progenitor cells into heart muscle, where some took on heart muscle cell markers.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseWt1(+) EPDC migration and cardiomyocyte marker expression
    Study

    mousen: not reported in source

    neonatal mice with apical resection
    Outcome group
    Heart repair and regeneration · Cardiovascular
    Studied dose
    Dose not stated · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    During the process of regeneration, Wt1(+) EPDCs migrated into myocardial region and some of them expressed Islet1 and the markers for mature cardiomyocytes, such as cTnT and SαA.
    1 context sentence
    Summary · context
    We intraperitoneally injected Tβ4 into 1-day-old mice on daily basis and then apical resection was performed on the mice 7 days later.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In Tβ4-treated neonatal mouse hearts, Tβ4 had no essential effect on cell cycle activity and no disruption of actin filaments was observed.

    In plain wordsIn mice, thymosin beta‑4 had no major effect on cell cycle activity and did not disrupt actin filaments in the heart.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeCell cycle activity and actin filaments
    Study

    mousen: not reported in source

    neonatal mice with apical resection
    Comparator
    PBS control
    Outcome group
    Heart repair and regeneration · Cardiovascular
    Studied dose
    Dose not stated · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    Tβ4 has no essential effect on cell cycle activity as no disruption of actin filaments was observed in Tβ4-treated hearts.
    1 context sentence
    Summary · context
    We intraperitoneally injected Tβ4 into 1-day-old mice on daily basis and then apical resection was performed on the mice 7 days later.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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